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• W4306894185 abstract "We have developed a novel urea-inducible recombinant protein production system by exploiting the Proteus mirabilis urease ureR-ureD promoter region and the ureR AraC-family transcriptional regulator. Experiments using the expression of β-galactosidase and green fluorescent protein (GFP) showed that promoter activity is tightly regulated and that varying the concentration of urea can give up to 100-fold induction. Production of proteins of biopharmaceutical interest has been demonstrated, including human growth hormone (hGH), a single chain antibody fragment (scFv) against interleukin-1β and a potential Neisserial vaccine candidate (BamAENm). Expression levels can be fine-tuned by temperature and different urea concentrations, and can be induced with readily available garden fertilisers and even urine. As urea is an inexpensive, stable inducer, a urea-induced expression system has the potential to considerably reduce the costs of large-scale recombinant protein production." @default.
• W4306894185 created "2022-10-21" @default.
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• W4306894185 date "2022-12-01" @default.
• W4306894185 modified "2023-10-14" @default.
• W4306894185 title "New vectors for urea-inducible recombinant protein production" @default.
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• W4306894185 doi "https://doi.org/10.1016/j.nbt.2022.10.003" @default.
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