FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306920744> ?p ?o ?g. }

• W4306920744 endingPage "10624" @default.
• W4306920744 startingPage "10624" @default.
• W4306920744 abstract "The Food and Drug Administration (FDA) has approved duvelisib (DUV) for managing follicular lymphoma, small lymphocytic lymphoma, and relapsed or refractory chronic lymphocytic leukemia. Seliciclib (SEL) is a candidate drug for these cancers, neurodegenerative disorders, renal diseases, several viral infections, and chronic inflammation disorders. This work describes the development and validation of a 96-microwell-based spectrophotometric method (MW-SPM) and a high-performance liquid chromatography with fluorescence detection method (HPLC-FD) for the quantitation of DUV and SEL in their bulk forms and capsules. The MW-SPM is based on the formation of colored charge transfer complexes (CTCs) as products for the reactions of DUV and SEL, as n-electron donors, with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), as a π-electron acceptor. The absorption intensity of the CTCs was measured by using an absorbance plate reader at 450 nm. The stoichiometric ratios of DUV:DDQ and SEL:DDQ were 1:1 and 1:2, respectively, and accordingly the reaction mechanisms were postulated. The HPLC-FD involved the chromatographic separation of DUV and SEL on a Hypersil™ Phenyl HPLC column (250 mm length × 4.6 mm i.d., 5 μm particle diameter) with a mobile phase composed of acetonitrile:acetate buffer, pH 4.5 (35:65, v/v) at a flow rate of 2.2 mL/min. DUV and SEL were detected at 370 nm after excitation at 280 nm. SEL was used as an internal standard (IS) for quantitation of DUV, and DUV was used as an IS for quantitation of SEL. Both MW-SPM and HPLC-FD were validated according to the guidelines of the International Council for Harmonization (ICH) for validation of analytical procedures. The linear ranges for both DUV and SEL were 14.52–200 µg/well (100 µL) and 0.12–3.2 µg/mL for MW-SPM and HPLC-FD, respectively. LOD values in MW-SPM for DUV and SEL were 4.4 and 3.17 µg/well, respectively; however, those for HPLC-FD were 0.03 and 0.05 µg/mL, respectively. The accuracy and precision of both methods were confirmed as the recovery values were ≥98.5% and the values of relative standard deviations (RSD) were ≤2.41%. Both methods were satisfactorily applied to the quantitation of DUV and SEL in their capsules; the mean recovery values were ≥99.2%. Both methods have simple procedures and high analytical throughput. Moreover, they consume a small volume of organic solvent; thus, they are economic and eco-friendly. Accordingly, the methods are valuable for routine use in quality control (QC) laboratories for quantitation of DUV and SEL in their bulk forms and capsules." @default.
• W4306920744 created "2022-10-21" @default.
• W4306920744 creator A5006706053 @default.
• W4306920744 creator A5013334740 @default.
• W4306920744 creator A5028013109 @default.
• W4306920744 creator A5052817318 @default.
• W4306920744 creator A5069114214 @default.
• W4306920744 creator A5075490553 @default.
• W4306920744 date "2022-10-20" @default.
• W4306920744 modified "2023-09-30" @default.
• W4306920744 title "Development and Validation of 96-Microwell-Based Spectrophotometric and High-Performance Liquid Chromatography with Fluorescence Detection Methods with High Throughput for Quantitation of Duvelisib and Seliciclib in Their Bulk Forms and Capsules" @default.
• W4306920744 cites W109446774 @default.
• W4306920744 cites W1939342789 @default.
• W4306920744 cites W1988506210 @default.
• W4306920744 cites W2007784749 @default.
• W4306920744 cites W2009627159 @default.
• W4306920744 cites W2012319099 @default.
• W4306920744 cites W2031854329 @default.
• W4306920744 cites W2038625523 @default.
• W4306920744 cites W2045998243 @default.
• W4306920744 cites W204656300 @default.
• W4306920744 cites W2054460119 @default.
• W4306920744 cites W2063096444 @default.
• W4306920744 cites W2089353165 @default.
• W4306920744 cites W2104740757 @default.
• W4306920744 cites W2104851203 @default.
• W4306920744 cites W2128956884 @default.
• W4306920744 cites W2141137646 @default.
• W4306920744 cites W2150840854 @default.
• W4306920744 cites W2160416801 @default.
• W4306920744 cites W2342717659 @default.
• W4306920744 cites W2588128517 @default.
• W4306920744 cites W2775670706 @default.
• W4306920744 cites W2789493946 @default.
• W4306920744 cites W2901488820 @default.
• W4306920744 cites W2902386805 @default.
• W4306920744 cites W2908831177 @default.
• W4306920744 cites W3025840807 @default.
• W4306920744 cites W3097486763 @default.
• W4306920744 cites W3128658305 @default.
• W4306920744 cites W3134707338 @default.
• W4306920744 cites W3145046568 @default.
• W4306920744 cites W3176928885 @default.
• W4306920744 cites W3194420070 @default.
• W4306920744 cites W4254865641 @default.
• W4306920744 doi "https://doi.org/10.3390/app122010624" @default.
• W4306920744 hasPublicationYear "2022" @default.
• W4306920744 type Work @default.
• W4306920744 citedByCount "0" @default.
• W4306920744 crossrefType "journal-article" @default.
• W4306920744 hasAuthorship W4306920744A5006706053 @default.
• W4306920744 hasAuthorship W4306920744A5013334740 @default.
• W4306920744 hasAuthorship W4306920744A5028013109 @default.
• W4306920744 hasAuthorship W4306920744A5052817318 @default.
• W4306920744 hasAuthorship W4306920744A5069114214 @default.
• W4306920744 hasAuthorship W4306920744A5075490553 @default.
• W4306920744 hasBestOaLocation W43069207441 @default.
• W4306920744 hasConcept C113196181 @default.
• W4306920744 hasConcept C179998833 @default.
• W4306920744 hasConcept C185592680 @default.
• W4306920744 hasConcept C2777463227 @default.
• W4306920744 hasConcept C43617362 @default.
• W4306920744 hasConceptScore W4306920744C113196181 @default.
• W4306920744 hasConceptScore W4306920744C179998833 @default.
• W4306920744 hasConceptScore W4306920744C185592680 @default.
• W4306920744 hasConceptScore W4306920744C2777463227 @default.
• W4306920744 hasConceptScore W4306920744C43617362 @default.
• W4306920744 hasIssue "20" @default.
• W4306920744 hasLocation W43069207441 @default.
• W4306920744 hasOpenAccess W4306920744 @default.
• W4306920744 hasPrimaryLocation W43069207441 @default.
• W4306920744 hasRelatedWork W1979512650 @default.
• W4306920744 hasRelatedWork W1995375582 @default.
• W4306920744 hasRelatedWork W2022476004 @default.
• W4306920744 hasRelatedWork W2056980537 @default.
• W4306920744 hasRelatedWork W2085491095 @default.
• W4306920744 hasRelatedWork W2119552130 @default.
• W4306920744 hasRelatedWork W2135763649 @default.
• W4306920744 hasRelatedWork W2256031060 @default.
• W4306920744 hasRelatedWork W3138239113 @default.
• W4306920744 hasRelatedWork W66516859 @default.
• W4306920744 hasVolume "12" @default.
• W4306920744 isParatext "false" @default.
• W4306920744 isRetracted "false" @default.
• W4306920744 workType "article" @default.