FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306937142> ?p ?o ?g. }

• W4306937142 endingPage "127" @default.
• W4306937142 startingPage "121" @default.
• W4306937142 abstract "Background . Atherosclerosis is one of the leading cardiovascular pathologies. Evidence suggests that DNA damage caused by endothelial cell exposure to mitomycin C (MMC) leads to endothelial dysfunction and is the risk factor for this disease. MMC is an alkylating mutagen involved in the development of oxidative stress, which is also a risk factor for atherosclerosis. Aim. To access the levels of oxidative stress markers in the primary human endothelial cell culture exposed to alkylating mutagen MMC. Material and Methods . Commercially available primary cultures of endothelial cells obtained from human coronary artery (HCAEC) and human internal thoracic artery (HITAEC) were used in the study. The cells were cultivated in the presence of 500 ng/mL MMC (experimental group) and without mutagenic load (control group). The levels of reactive oxygen species, reactive nitrogen species, and 8-OH-deoxyguanosine (8-OHdG) in cell growth media were assessed by enzyme-linked immunosorbent assay. The relative telomere length and expression of TERT and POT1 genes were accessed in endothelial cells by quantitative polymerase chain reaction. Statistical analysis of data was performed using GraphPad Prism 9 software. Results . There were no differences in the concentrations of reactive oxygen species, reactive nitrogen species (NO2 -, NO3 -, NO2 -/NO3 -), and 8-OHdG in HCAEC and HITAEC cultures exposed to MMC compared to the corresponding parameters in the non-exposed controls. At the same time, HCAEC and HITAEC exposed to MMC were characterized by a decrease in the relative telomere length compared to control (10.97 vs. 27.03 in HCAEC, p = 0.002 and 9.12 vs. 25.64 in HITAEC, p = 0.001). Moreover, we discovered 1.75-fold increase in the expression of POT1 gene in the experimental HCAEC compared to control (p = 0.019). No expression of TERT gene was observed in study groups. Conclusions . Alkylating mutagen MMC did not induce any pronounced oxidative stress in the primary human endothelial cells in vitro. The development of endothelial dysfunction caused by MMC exposure was triggered mainly by DNA alkylation resulting in the genotoxic stress in the endothelial cells." @default.
• W4306937142 created "2022-10-21" @default.
• W4306937142 creator A5021400982 @default.
• W4306937142 creator A5026702567 @default.
• W4306937142 creator A5029764642 @default.
• W4306937142 creator A5030990869 @default.
• W4306937142 creator A5044335799 @default.
• W4306937142 creator A5057508036 @default.
• W4306937142 date "2022-10-20" @default.
• W4306937142 modified "2023-10-01" @default.
• W4306937142 title "Oxidative stress in the endothelial cell culture exposed to mitomycin C" @default.
• W4306937142 cites W1977632395 @default.
• W4306937142 cites W1993576997 @default.
• W4306937142 cites W2002637963 @default.
• W4306937142 cites W2003399770 @default.
• W4306937142 cites W2006315712 @default.
• W4306937142 cites W2011804396 @default.
• W4306937142 cites W2013322023 @default.
• W4306937142 cites W2017158556 @default.
• W4306937142 cites W2031304365 @default.
• W4306937142 cites W2139666789 @default.
• W4306937142 cites W2160621707 @default.
• W4306937142 cites W2168420558 @default.
• W4306937142 cites W2568928300 @default.
• W4306937142 cites W2738129216 @default.
• W4306937142 cites W2755569277 @default.
• W4306937142 cites W2899736836 @default.
• W4306937142 cites W3038684839 @default.
• W4306937142 cites W3155824129 @default.
• W4306937142 cites W3156531835 @default.
• W4306937142 cites W3176475084 @default.
• W4306937142 doi "https://doi.org/10.29001/2073-8552-2022-37-3-121-127" @default.
• W4306937142 hasPublicationYear "2022" @default.
• W4306937142 type Work @default.
• W4306937142 citedByCount "0" @default.
• W4306937142 crossrefType "journal-article" @default.
• W4306937142 hasAuthorship W4306937142A5021400982 @default.
• W4306937142 hasAuthorship W4306937142A5026702567 @default.
• W4306937142 hasAuthorship W4306937142A5029764642 @default.
• W4306937142 hasAuthorship W4306937142A5030990869 @default.
• W4306937142 hasAuthorship W4306937142A5044335799 @default.
• W4306937142 hasAuthorship W4306937142A5057508036 @default.
• W4306937142 hasBestOaLocation W43069371421 @default.
• W4306937142 hasConcept C114246631 @default.
• W4306937142 hasConcept C123012128 @default.
• W4306937142 hasConcept C134018914 @default.
• W4306937142 hasConcept C143425029 @default.
• W4306937142 hasConcept C153911025 @default.
• W4306937142 hasConcept C16685009 @default.
• W4306937142 hasConcept C202751555 @default.
• W4306937142 hasConcept C203014093 @default.
• W4306937142 hasConcept C2776151105 @default.
• W4306937142 hasConcept C2776639595 @default.
• W4306937142 hasConcept C2779338040 @default.
• W4306937142 hasConcept C2780972559 @default.
• W4306937142 hasConcept C48349386 @default.
• W4306937142 hasConcept C54355233 @default.
• W4306937142 hasConcept C552990157 @default.
• W4306937142 hasConcept C55493867 @default.
• W4306937142 hasConcept C71924100 @default.
• W4306937142 hasConcept C86803240 @default.
• W4306937142 hasConcept C98274493 @default.
• W4306937142 hasConceptScore W4306937142C114246631 @default.
• W4306937142 hasConceptScore W4306937142C123012128 @default.
• W4306937142 hasConceptScore W4306937142C134018914 @default.
• W4306937142 hasConceptScore W4306937142C143425029 @default.
• W4306937142 hasConceptScore W4306937142C153911025 @default.
• W4306937142 hasConceptScore W4306937142C16685009 @default.
• W4306937142 hasConceptScore W4306937142C202751555 @default.
• W4306937142 hasConceptScore W4306937142C203014093 @default.
• W4306937142 hasConceptScore W4306937142C2776151105 @default.
• W4306937142 hasConceptScore W4306937142C2776639595 @default.
• W4306937142 hasConceptScore W4306937142C2779338040 @default.
• W4306937142 hasConceptScore W4306937142C2780972559 @default.
• W4306937142 hasConceptScore W4306937142C48349386 @default.
• W4306937142 hasConceptScore W4306937142C54355233 @default.
• W4306937142 hasConceptScore W4306937142C552990157 @default.
• W4306937142 hasConceptScore W4306937142C55493867 @default.
• W4306937142 hasConceptScore W4306937142C71924100 @default.
• W4306937142 hasConceptScore W4306937142C86803240 @default.
• W4306937142 hasConceptScore W4306937142C98274493 @default.
• W4306937142 hasIssue "3" @default.
• W4306937142 hasLocation W43069371421 @default.
• W4306937142 hasOpenAccess W4306937142 @default.
• W4306937142 hasPrimaryLocation W43069371421 @default.
• W4306937142 hasRelatedWork W143687651 @default.
• W4306937142 hasRelatedWork W2004624647 @default.
• W4306937142 hasRelatedWork W2061336798 @default.
• W4306937142 hasRelatedWork W2084408440 @default.
• W4306937142 hasRelatedWork W2591642597 @default.
• W4306937142 hasRelatedWork W2803551820 @default.
• W4306937142 hasRelatedWork W4220752736 @default.
• W4306937142 hasRelatedWork W4311130491 @default.
• W4306937142 hasRelatedWork W2199579455 @default.
• W4306937142 hasRelatedWork W3091876869 @default.
• W4306937142 hasVolume "37" @default.
• W4306937142 isParatext "false" @default.
• W4306937142 isRetracted "false" @default.

• next