Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307002308> ?p ?o ?g. }
- W4307002308 endingPage "e0276657" @default.
- W4307002308 startingPage "e0276657" @default.
- W4307002308 abstract "Sparse labeling of individual cells is an important approach in neuroscience and many other fields of research. Various methods have been developed to sparsely label only a small population of cells; however, there is no simple and reproducible strategy for managing the probability of sparse labeling at desired levels. Here, we aimed to develop a novel methodology based on the Cre-lox system to regulate sparseness at desired levels, and we purely analyzed cleavage efficiencies of loxP mutants by Cre. We hypothesized that mutations in the loxP sequence reduce the recognition efficiency by Cre, which enables the regulation of the sparseness level of gene expression. In this research, we mutagenized the loxP sequence and analyzed a library of loxP variants. We evaluated more than 1000 mutant loxP sequences, including mutants with reduced excision efficiencies by Cre ranging from 0.51% to 59%. This result suggests that these mutant loxP sequences can be useful in regulating the sparseness of genetic labeling at desired levels." @default.
- W4307002308 created "2022-10-22" @default.
- W4307002308 creator A5006618132 @default.
- W4307002308 creator A5029132443 @default.
- W4307002308 creator A5063620609 @default.
- W4307002308 creator A5066036279 @default.
- W4307002308 creator A5074110362 @default.
- W4307002308 date "2022-10-21" @default.
- W4307002308 modified "2023-10-16" @default.
- W4307002308 title "Evaluation of a library of loxP variants with a wide range of recombination efficiencies by Cre" @default.
- W4307002308 cites W1538926863 @default.
- W4307002308 cites W1556784368 @default.
- W4307002308 cites W1586539673 @default.
- W4307002308 cites W1966084915 @default.
- W4307002308 cites W1976228047 @default.
- W4307002308 cites W1983188063 @default.
- W4307002308 cites W1983223950 @default.
- W4307002308 cites W1989244157 @default.
- W4307002308 cites W1990992825 @default.
- W4307002308 cites W2002581509 @default.
- W4307002308 cites W2006229889 @default.
- W4307002308 cites W2011949330 @default.
- W4307002308 cites W2015977442 @default.
- W4307002308 cites W2020777893 @default.
- W4307002308 cites W2022846914 @default.
- W4307002308 cites W2031636461 @default.
- W4307002308 cites W2032964168 @default.
- W4307002308 cites W2042742511 @default.
- W4307002308 cites W2043351945 @default.
- W4307002308 cites W2046163105 @default.
- W4307002308 cites W2056928450 @default.
- W4307002308 cites W2057231177 @default.
- W4307002308 cites W2063552704 @default.
- W4307002308 cites W2080122841 @default.
- W4307002308 cites W2091467671 @default.
- W4307002308 cites W2094853557 @default.
- W4307002308 cites W2095310293 @default.
- W4307002308 cites W2099863332 @default.
- W4307002308 cites W2104854307 @default.
- W4307002308 cites W2104975387 @default.
- W4307002308 cites W2110232607 @default.
- W4307002308 cites W2117577687 @default.
- W4307002308 cites W2119095571 @default.
- W4307002308 cites W2123311560 @default.
- W4307002308 cites W2127810309 @default.
- W4307002308 cites W2135259468 @default.
- W4307002308 cites W2147987903 @default.
- W4307002308 cites W2166670234 @default.
- W4307002308 cites W2166700927 @default.
- W4307002308 cites W2231058499 @default.
- W4307002308 cites W2464464964 @default.
- W4307002308 cites W2516559527 @default.
- W4307002308 cites W2581259096 @default.
- W4307002308 cites W2591812992 @default.
- W4307002308 cites W2769642916 @default.
- W4307002308 cites W2793395990 @default.
- W4307002308 cites W2804640708 @default.
- W4307002308 cites W2887185581 @default.
- W4307002308 cites W2900358948 @default.
- W4307002308 cites W2951112007 @default.
- W4307002308 cites W3002301481 @default.
- W4307002308 cites W3010845844 @default.
- W4307002308 cites W3043631840 @default.
- W4307002308 cites W3048685979 @default.
- W4307002308 cites W3091274460 @default.
- W4307002308 cites W4211242168 @default.
- W4307002308 cites W4232906488 @default.
- W4307002308 doi "https://doi.org/10.1371/journal.pone.0276657" @default.
- W4307002308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36269789" @default.
- W4307002308 hasPublicationYear "2022" @default.
- W4307002308 type Work @default.
- W4307002308 citedByCount "1" @default.
- W4307002308 countsByYear W43070023082023 @default.
- W4307002308 crossrefType "journal-article" @default.
- W4307002308 hasAuthorship W4307002308A5006618132 @default.
- W4307002308 hasAuthorship W4307002308A5029132443 @default.
- W4307002308 hasAuthorship W4307002308A5063620609 @default.
- W4307002308 hasAuthorship W4307002308A5066036279 @default.
- W4307002308 hasAuthorship W4307002308A5074110362 @default.
- W4307002308 hasBestOaLocation W43070023081 @default.
- W4307002308 hasConcept C102230213 @default.
- W4307002308 hasConcept C104317684 @default.
- W4307002308 hasConcept C125929170 @default.
- W4307002308 hasConcept C141035611 @default.
- W4307002308 hasConcept C143065580 @default.
- W4307002308 hasConcept C144024400 @default.
- W4307002308 hasConcept C149923435 @default.
- W4307002308 hasConcept C2778112365 @default.
- W4307002308 hasConcept C2908647359 @default.
- W4307002308 hasConcept C38027674 @default.
- W4307002308 hasConcept C54355233 @default.
- W4307002308 hasConcept C70721500 @default.
- W4307002308 hasConcept C86803240 @default.
- W4307002308 hasConceptScore W4307002308C102230213 @default.
- W4307002308 hasConceptScore W4307002308C104317684 @default.
- W4307002308 hasConceptScore W4307002308C125929170 @default.
- W4307002308 hasConceptScore W4307002308C141035611 @default.
- W4307002308 hasConceptScore W4307002308C143065580 @default.