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• W4307112388 endingPage "122036" @default.
• W4307112388 startingPage "122036" @default.
• W4307112388 abstract "Food poisoning is a gastrointestinal illness caused by food-borne enterotoxin produced by the bacterium Staphylococcus aureus. The effective dose of Staphylococcal enterotoxin 'B' (SEB) is estimated to be 0.4 ng/kg of body weight, whereas the 50 % lethal dose is found to be 20 ng/kg of body weight for humans exposed by the inhalation route. The present report highlights the development of a fluorescence resonance energy transfer (FRET) based assay for the detection of Staphylococcal enterotoxin. Highly fluorescent, aqueous quantum dots were synthesized and conjugated with Staphylococcal enterotoxin 'B' specific bioreceptors. SEB specific aptamer and SEB antibody were labeled with fluorescent quantum dots for recognizing and binding two separate epitopes in the SEB. A combination of two probes against different epitopic regions in a homogeneous sandwich assay format enhanced the sensitivity and specificity of SEB detection. In the presence of the enterotoxin, both the aptamer and antibody came in close proximity with each other and FRET was observed. A linear decrease in the fluorescence at 562 nm and a corresponding increase in the signal at 644 nm was observed with increasing concentrations of SEB, when excited at the absorption maximum of quantum dots. The limit of detection for the developed assay obtained was less than 1 ng/ml. The method was employed in apple juice and quantitated using Enzyme-linked Immunosorbent Assay (ELISA). The designed assay was rapid and robust and can be extrapolated as a platform for the detection of various disease-causing agents of biomedical significance." @default.
• W4307112388 created "2022-10-27" @default.
• W4307112388 creator A5018708728 @default.
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• W4307112388 date "2023-02-01" @default.
• W4307112388 modified "2023-09-24" @default.
• W4307112388 title "Femtomolar detection of staphylococcal enterotoxin ‘B’ using a fluorescent quantum dot based hybrid Apta-immunosensor" @default.
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• W4307112388 doi "https://doi.org/10.1016/j.saa.2022.122036" @default.
• W4307112388 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36327805" @default.
• W4307112388 hasPublicationYear "2023" @default.
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