FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307113344> ?p ?o ?g. }

• W4307113344 abstract "Improving the mechanical properties and angiogenesis of acellular scaffolds before transplantation is an important challenge facing the development of acellular liver grafts. The present study aimed to evaluate the cytotoxicity and angiogenesis of polyethylene glycol (PEG) crosslinked decellularized single liver lobe scaffolds (DLSs), and establish its suitability as a graft for long-term liver tissue engineering.Using mercaptoacrylate produced by the Michael addition reaction, DLSs were first modified using N-succinimidyl S-acetylthioacetate (SATA), followed by cross-linking with PEG as well as vascular endothelial growth factor (VEGF). The optimal concentration of agents and time of the individual steps were identified in this procedure through biomechanical testing and morphological analysis. Subsequently, human umbilical vein endothelial cells (HUVECs) were seeded on the PEG crosslinked scaffolds to detect the proliferation and viability of cells. The scaffolds were then transplanted into the subcutaneous tissue of Sprague-Dawley rats to evaluate angiogenesis. In addition, the average number of blood vessels was evaluated in the grafts with or without PEG at days 7, 14, and 21 after implantation.The PEG crosslinked DLS maintained their three-dimensional structure and were more translucent after decellularization than native DLS, which presented a denser and more porous network structure. The results for Young's modulus proved that the mechanical properties of 0.5 PEG crosslinked DLS were the best and close to that of native livers. The PEG-VEGF-DLS could better promote cell proliferation and differentiation of HUVECs compared with the groups without PEG cross-linking. Importantly, the average density of blood vessels was higher in the PEG-VEGF-DLS than that in other groups at days 7, 14, and 21 after implantation in vivo.The PEG crosslinked DLS with VEGF could improve the biomechanical properties of native DLS, and most importantly, their lack of cytotoxicity provides a new route to promote the proliferation of cells in vitro and angiogenesis in vivo in liver tissue engineering." @default.
• W4307113344 created "2022-10-27" @default.
• W4307113344 creator A5010270772 @default.
• W4307113344 creator A5029041901 @default.
• W4307113344 creator A5029481179 @default.
• W4307113344 creator A5050543532 @default.
• W4307113344 creator A5052585781 @default.
• W4307113344 creator A5054535062 @default.
• W4307113344 creator A5061895984 @default.
• W4307113344 creator A5077353403 @default.
• W4307113344 date "2022-10-01" @default.
• W4307113344 modified "2023-09-23" @default.
• W4307113344 title "Polyethylene glycol crosslinked decellularized single liver lobe scaffolds with vascular endothelial growth factor promotes angiogenesis in vivo" @default.
• W4307113344 cites W1441169201 @default.
• W4307113344 cites W1533363236 @default.
• W4307113344 cites W1916542938 @default.
• W4307113344 cites W1979901141 @default.
• W4307113344 cites W1980288137 @default.
• W4307113344 cites W2000658561 @default.
• W4307113344 cites W2002230244 @default.
• W4307113344 cites W2006247923 @default.
• W4307113344 cites W2035066344 @default.
• W4307113344 cites W2050150175 @default.
• W4307113344 cites W2085637918 @default.
• W4307113344 cites W2111017388 @default.
• W4307113344 cites W2170972054 @default.
• W4307113344 cites W2176000863 @default.
• W4307113344 cites W2261148770 @default.
• W4307113344 cites W2474510574 @default.
• W4307113344 cites W2747569984 @default.
• W4307113344 cites W2765271604 @default.
• W4307113344 cites W2795133409 @default.
• W4307113344 cites W2898390012 @default.
• W4307113344 cites W2904444744 @default.
• W4307113344 cites W2957112233 @default.
• W4307113344 cites W2958872567 @default.
• W4307113344 cites W2972206596 @default.
• W4307113344 cites W2972492746 @default.
• W4307113344 cites W2979432487 @default.
• W4307113344 cites W2985247083 @default.
• W4307113344 cites W2995535906 @default.
• W4307113344 cites W3006500690 @default.
• W4307113344 cites W3022100157 @default.
• W4307113344 cites W3034056485 @default.
• W4307113344 cites W3082498032 @default.
• W4307113344 cites W3086664918 @default.
• W4307113344 cites W3102771713 @default.
• W4307113344 cites W3110117427 @default.
• W4307113344 cites W3123006103 @default.
• W4307113344 cites W3141740989 @default.
• W4307113344 cites W3217301952 @default.
• W4307113344 doi "https://doi.org/10.1016/j.hbpd.2022.10.007" @default.
• W4307113344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36335030" @default.
• W4307113344 hasPublicationYear "2022" @default.
• W4307113344 type Work @default.
• W4307113344 citedByCount "2" @default.
• W4307113344 countsByYear W43071133442023 @default.
• W4307113344 crossrefType "journal-article" @default.
• W4307113344 hasAuthorship W4307113344A5010270772 @default.
• W4307113344 hasAuthorship W4307113344A5029041901 @default.
• W4307113344 hasAuthorship W4307113344A5029481179 @default.
• W4307113344 hasAuthorship W4307113344A5050543532 @default.
• W4307113344 hasAuthorship W4307113344A5052585781 @default.
• W4307113344 hasAuthorship W4307113344A5054535062 @default.
• W4307113344 hasAuthorship W4307113344A5061895984 @default.
• W4307113344 hasAuthorship W4307113344A5077353403 @default.
• W4307113344 hasConcept C10138342 @default.
• W4307113344 hasConcept C136229726 @default.
• W4307113344 hasConcept C141071460 @default.
• W4307113344 hasConcept C150903083 @default.
• W4307113344 hasConcept C162324750 @default.
• W4307113344 hasConcept C167734588 @default.
• W4307113344 hasConcept C185592680 @default.
• W4307113344 hasConcept C190672674 @default.
• W4307113344 hasConcept C202751555 @default.
• W4307113344 hasConcept C207001950 @default.
• W4307113344 hasConcept C2776964284 @default.
• W4307113344 hasConcept C2777025900 @default.
• W4307113344 hasConcept C2777411675 @default.
• W4307113344 hasConcept C2780394083 @default.
• W4307113344 hasConcept C2911091166 @default.
• W4307113344 hasConcept C49892992 @default.
• W4307113344 hasConcept C502942594 @default.
• W4307113344 hasConcept C54400483 @default.
• W4307113344 hasConcept C55493867 @default.
• W4307113344 hasConcept C71924100 @default.
• W4307113344 hasConcept C86803240 @default.
• W4307113344 hasConceptScore W4307113344C10138342 @default.
• W4307113344 hasConceptScore W4307113344C136229726 @default.
• W4307113344 hasConceptScore W4307113344C141071460 @default.
• W4307113344 hasConceptScore W4307113344C150903083 @default.
• W4307113344 hasConceptScore W4307113344C162324750 @default.
• W4307113344 hasConceptScore W4307113344C167734588 @default.
• W4307113344 hasConceptScore W4307113344C185592680 @default.
• W4307113344 hasConceptScore W4307113344C190672674 @default.
• W4307113344 hasConceptScore W4307113344C202751555 @default.
• W4307113344 hasConceptScore W4307113344C207001950 @default.
• W4307113344 hasConceptScore W4307113344C2776964284 @default.
• W4307113344 hasConceptScore W4307113344C2777025900 @default.
• W4307113344 hasConceptScore W4307113344C2777411675 @default.

• next