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- W4307238660 abstract "Abstract This study evaluated and determined the toxicity levels of glyphosate and its possible transformed leachates on prokaryotic and eukaryotic cells. Four different microcosms were used: undisturbed soil and chicken manure (SBCB), undisturbed soil and sterile chicken manure (SBCE), sterile soil and manure (SECE), sterilized soil and chicken manure (SECB) and a control group with undisturbed soil and chicken manure. Commercial glyphosate solution was applied at 3, 6 and 9 days of exposure. The quantification of glyphosate for each leachate was evaluated by the HPLC technique. A final concentration of 54.80 ppm was found in the SECE microcosm at 9 days of exposure. Furthermore, a mutation assay demonstrated mutagenic activity by the herbicide, which increased in the microcosm SBCB after 9 days of exposure to glyphosate. A 70% of mutagenic action. The cytotoxicity determined in HUVEC cells showed that the SECE microcosm reduced its viability by 66% at 9 days of exposure. Likewise, the microcosm SECE 65% at 6 days, SECB 63% at 9 days and SECB 59% at 6 days of exposure. A 16s rDNA molecular analysis concluded that the most representative phylum were: Firmicutes (54.55%) and Proteobacteria (18.18%), followed by a fair group of Actinobacteria (9.09%) and Fusobacteria (9.09%)" @default.
- W4307238660 created "2022-10-30" @default.
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- W4307238660 date "2022-10-25" @default.
- W4307238660 modified "2023-09-30" @default.
- W4307238660 title "Evaluation of n-phosphonomethyl glycine toxicity in non-disturbed soil leaches" @default.
- W4307238660 doi "https://doi.org/10.21203/rs.3.rs-2062495/v1" @default.
- W4307238660 hasPublicationYear "2022" @default.
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