FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307309323> ?p ?o ?g. }

• W4307309323 abstract "Neuron-immune interaction through secreted factors contributes significantly to the complex microenvironment in the central nervous system that could alter cell functionalities and fates in both physiological and pathological conditions, which remains poorly characterized at the single-cell level. Herein, using a spatially patterned antibody barcode microchip, we realized the mapping of 12 different secretomes, covering cytokines, neurotrophic factors (NFs), and neuron-derived exosomes (NDEs) from high-throughput, paired single cells (≥ 600) simultaneously under normal conditions and an Alzheimer’s disease (AD) model induced with amyloid beta protein 1-42 (Aβ 1–42 ). We applied the platform to analyze the secretion profiles from paired neuron–macrophage and neuron–microglia single cells with human cell lines. We found that pairwise neuron–macrophage interaction would trigger immune responses and attenuate neuron cells’ secretion, while neuron–microglia interaction generally results in opposite outcomes in secretion. When neuron cells are induced with Aβ 1–42 protein into the AD model, both neuron–macrophage and neuron–microglia interactions lead to increased cytokines and NDEs and decreased NFs. Further analysis of AD patients’ serum showed that NDEs were significantly higher in patients’ samples than in the control group, validating our observation from the interaction assay. Furthermore, we resolved previously undifferentiated heterogeneity underlying the secretions from single-neuron cells. We found that the NDE and NF secretion was less dependent on the paracrine signaling between one another and that secretions from neuron cells would attenuate after differentiation with Aβ 1–42 . This study demonstrates the mapping of the different secretomes from paired neuron-immune single cells, providing avenues for understanding how neurons and immune cells interact through the complex secretome network." @default.
• W4307309323 created "2022-10-31" @default.
• W4307309323 creator A5005058788 @default.
• W4307309323 creator A5008141480 @default.
• W4307309323 creator A5023246860 @default.
• W4307309323 creator A5027059038 @default.
• W4307309323 creator A5035413568 @default.
• W4307309323 creator A5038137713 @default.
• W4307309323 creator A5041238117 @default.
• W4307309323 creator A5048420289 @default.
• W4307309323 creator A5055805718 @default.
• W4307309323 creator A5076042825 @default.
• W4307309323 creator A5082175210 @default.
• W4307309323 date "2022-10-26" @default.
• W4307309323 modified "2023-10-17" @default.
• W4307309323 title "Mapping secretome-mediated interaction between paired neuron–macrophage single cells" @default.
• W4307309323 cites W1566386955 @default.
• W4307309323 cites W1841850981 @default.
• W4307309323 cites W1969714939 @default.
• W4307309323 cites W1981764021 @default.
• W4307309323 cites W1982729887 @default.
• W4307309323 cites W1995037165 @default.
• W4307309323 cites W2026528573 @default.
• W4307309323 cites W2033617297 @default.
• W4307309323 cites W2051452288 @default.
• W4307309323 cites W2055823139 @default.
• W4307309323 cites W2058669152 @default.
• W4307309323 cites W2065236536 @default.
• W4307309323 cites W2067267561 @default.
• W4307309323 cites W2085435800 @default.
• W4307309323 cites W2088253641 @default.
• W4307309323 cites W2090661905 @default.
• W4307309323 cites W2098259366 @default.
• W4307309323 cites W2108398194 @default.
• W4307309323 cites W2124836944 @default.
• W4307309323 cites W2138868558 @default.
• W4307309323 cites W2144342921 @default.
• W4307309323 cites W2144447780 @default.
• W4307309323 cites W2154575244 @default.
• W4307309323 cites W2168204150 @default.
• W4307309323 cites W2172864295 @default.
• W4307309323 cites W2255996476 @default.
• W4307309323 cites W2572710398 @default.
• W4307309323 cites W2581824660 @default.
• W4307309323 cites W2620901112 @default.
• W4307309323 cites W2737946846 @default.
• W4307309323 cites W2770068166 @default.
• W4307309323 cites W2889080358 @default.
• W4307309323 cites W2891459456 @default.
• W4307309323 cites W2905201183 @default.
• W4307309323 cites W2910148804 @default.
• W4307309323 cites W2911725378 @default.
• W4307309323 cites W2914756534 @default.
• W4307309323 cites W2942678593 @default.
• W4307309323 cites W2950460603 @default.
• W4307309323 cites W2951560273 @default.
• W4307309323 cites W2952272517 @default.
• W4307309323 cites W2959379447 @default.
• W4307309323 cites W2988958689 @default.
• W4307309323 cites W2994525341 @default.
• W4307309323 cites W2996373709 @default.
• W4307309323 cites W3030497550 @default.
• W4307309323 cites W3044240100 @default.
• W4307309323 cites W3086786978 @default.
• W4307309323 cites W3099284546 @default.
• W4307309323 cites W3107395714 @default.
• W4307309323 cites W3107630607 @default.
• W4307309323 cites W3129679664 @default.
• W4307309323 cites W3163019925 @default.
• W4307309323 cites W3196827409 @default.
• W4307309323 cites W3200466182 @default.
• W4307309323 cites W3205323262 @default.
• W4307309323 doi "https://doi.org/10.1073/pnas.2200944119" @default.
• W4307309323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36288285" @default.
• W4307309323 hasPublicationYear "2022" @default.
• W4307309323 type Work @default.
• W4307309323 citedByCount "3" @default.
• W4307309323 countsByYear W43073093232023 @default.
• W4307309323 crossrefType "journal-article" @default.
• W4307309323 hasAuthorship W4307309323A5005058788 @default.
• W4307309323 hasAuthorship W4307309323A5008141480 @default.
• W4307309323 hasAuthorship W4307309323A5023246860 @default.
• W4307309323 hasAuthorship W4307309323A5027059038 @default.
• W4307309323 hasAuthorship W4307309323A5035413568 @default.
• W4307309323 hasAuthorship W4307309323A5038137713 @default.
• W4307309323 hasAuthorship W4307309323A5041238117 @default.
• W4307309323 hasAuthorship W4307309323A5048420289 @default.
• W4307309323 hasAuthorship W4307309323A5055805718 @default.
• W4307309323 hasAuthorship W4307309323A5076042825 @default.
• W4307309323 hasAuthorship W4307309323A5082175210 @default.
• W4307309323 hasBestOaLocation W43073093231 @default.
• W4307309323 hasConcept C104317684 @default.
• W4307309323 hasConcept C145059251 @default.
• W4307309323 hasConcept C1491633281 @default.
• W4307309323 hasConcept C160539049 @default.
• W4307309323 hasConcept C169760540 @default.
• W4307309323 hasConcept C170493617 @default.
• W4307309323 hasConcept C202751555 @default.
• W4307309323 hasConcept C203014093 @default.
• W4307309323 hasConcept C20518536 @default.

• next