Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307340087> ?p ?o ?g. }
- W4307340087 endingPage "358" @default.
- W4307340087 startingPage "343" @default.
- W4307340087 abstract "Environmental cyanotoxin exposure may be a trigger of testicular cancer. Activation of PI3K/AKT/mTOR signaling pathway is the critical molecular event in testicular carcinogenesis. As a widespread cyanotoxin, microcystin-leucine arginine (MC-LR) is known to induce cell malignant transformation and tumorigenesis. However, the effects of MC-LR on the regulatory mechanism of PI3K/AKT/mTOR pathway in seminoma, the most common testicular tumor, are unknown. In this study, mouse spermatogonia cell line (GC-1) and nude mice were used to investigate the effects and mechanisms of MC-LR on the malignant transformation of spermatogonia by nude mouse tumorigenesis assay, cell migration invasion assay, western blot, and cell cycle assay, and so forth. The results showed that, after continuous exposure to environmentally relevant concentrations of MC-LR (20 nM) for 35 generations, the proliferation, migration, and invasion abilities of GC-1 cells were increased by 120%, 340%, and 370%, respectively. In nude mice, MC-LR-treated GC-1 cells formed tumors with significantly greater volume (0.998 ± 0.768 cm3 ) and weight (0.637 ± 0.406 g) than the control group (0.067 ± 0.039 cm3 ; 0.094 ± 0.087 g) (P < .05). Furthermore, PI3K inhibitor Wortmannin inhibited the PI3K/AKT/mTOR pathway and its downstream proteins (c-MYC, CDK4, CCND1, and MMP14) activated by MC-LR. Blocking PI3K alleviated MC-LR-induced cell cycle disorder and malignant proliferation, migration and invasive of GC-1 cells. Altogether, our findings suggest that MC-LR can induce malignant transformation of mouse spermatogonia, and the PI3K/AKT/mTOR pathway-mediated cell cycle dysregulation may be an important target for malignant proliferation. This study provides clues to further reveal the etiology and pathogenesis of seminoma." @default.
- W4307340087 created "2022-10-31" @default.
- W4307340087 creator A5004405647 @default.
- W4307340087 creator A5023472188 @default.
- W4307340087 creator A5031458979 @default.
- W4307340087 creator A5032378609 @default.
- W4307340087 creator A5039489060 @default.
- W4307340087 creator A5049166005 @default.
- W4307340087 creator A5054775481 @default.
- W4307340087 creator A5057115300 @default.
- W4307340087 creator A5058708276 @default.
- W4307340087 creator A5059409198 @default.
- W4307340087 creator A5083130438 @default.
- W4307340087 date "2022-10-26" @default.
- W4307340087 modified "2023-10-18" @default.
- W4307340087 title "<scp>PI3K</scp>/<scp>AKT</scp>/<scp>mTOR</scp> pathway mediated‐cell cycle dysregulation contribute to malignant proliferation of mouse spermatogonia induced by microcystin‐leucine arginine" @default.
- W4307340087 cites W1505669143 @default.
- W4307340087 cites W1935001250 @default.
- W4307340087 cites W1969048724 @default.
- W4307340087 cites W1978671354 @default.
- W4307340087 cites W1979860922 @default.
- W4307340087 cites W1980426808 @default.
- W4307340087 cites W1996283037 @default.
- W4307340087 cites W2000211667 @default.
- W4307340087 cites W2001721732 @default.
- W4307340087 cites W2002680755 @default.
- W4307340087 cites W2006337111 @default.
- W4307340087 cites W2007460428 @default.
- W4307340087 cites W2008257836 @default.
- W4307340087 cites W2019292081 @default.
- W4307340087 cites W2029175358 @default.
- W4307340087 cites W2041766504 @default.
- W4307340087 cites W2049733012 @default.
- W4307340087 cites W2054334092 @default.
- W4307340087 cites W2063060894 @default.
- W4307340087 cites W2071164327 @default.
- W4307340087 cites W2074513068 @default.
- W4307340087 cites W2077012992 @default.
- W4307340087 cites W2082184681 @default.
- W4307340087 cites W2088440273 @default.
- W4307340087 cites W2097762091 @default.
- W4307340087 cites W2103102722 @default.
- W4307340087 cites W2106969896 @default.
- W4307340087 cites W2110629976 @default.
- W4307340087 cites W2127067110 @default.
- W4307340087 cites W2156119927 @default.
- W4307340087 cites W2165322824 @default.
- W4307340087 cites W2235768445 @default.
- W4307340087 cites W2300851634 @default.
- W4307340087 cites W2518718875 @default.
- W4307340087 cites W2532183954 @default.
- W4307340087 cites W2606513998 @default.
- W4307340087 cites W2608352093 @default.
- W4307340087 cites W2611938952 @default.
- W4307340087 cites W2620858295 @default.
- W4307340087 cites W2621427899 @default.
- W4307340087 cites W2738146320 @default.
- W4307340087 cites W2744377419 @default.
- W4307340087 cites W2760952514 @default.
- W4307340087 cites W2904352309 @default.
- W4307340087 cites W2907722642 @default.
- W4307340087 cites W2913667153 @default.
- W4307340087 cites W2927296218 @default.
- W4307340087 cites W2929947297 @default.
- W4307340087 cites W2943470918 @default.
- W4307340087 cites W2944859742 @default.
- W4307340087 cites W2963004843 @default.
- W4307340087 cites W2970922664 @default.
- W4307340087 cites W2972983732 @default.
- W4307340087 cites W2973188102 @default.
- W4307340087 cites W2974238339 @default.
- W4307340087 cites W2977702609 @default.
- W4307340087 cites W2979668391 @default.
- W4307340087 cites W2979808541 @default.
- W4307340087 cites W2986530477 @default.
- W4307340087 cites W3001128483 @default.
- W4307340087 cites W3016718031 @default.
- W4307340087 cites W3034493637 @default.
- W4307340087 cites W3046745751 @default.
- W4307340087 cites W3047962480 @default.
- W4307340087 cites W3086494806 @default.
- W4307340087 cites W3091067702 @default.
- W4307340087 cites W3092405734 @default.
- W4307340087 cites W3107835337 @default.
- W4307340087 cites W3112188931 @default.
- W4307340087 cites W3128009477 @default.
- W4307340087 cites W3132250580 @default.
- W4307340087 cites W3134227672 @default.
- W4307340087 cites W3134813985 @default.
- W4307340087 cites W3135283094 @default.
- W4307340087 cites W3136175339 @default.
- W4307340087 cites W3138885187 @default.
- W4307340087 cites W3177684048 @default.
- W4307340087 cites W3213971824 @default.
- W4307340087 cites W4205632882 @default.
- W4307340087 cites W4205938412 @default.
- W4307340087 cites W4211010233 @default.
- W4307340087 cites W4283120505 @default.