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• W4307407874 abstract "This study describes the structure of DNA polymerase I from Thermus phage G20c, termed PolI_G20c. This is the first structure of a DNA polymerase originating from a group of related thermophilic bacteriophages infecting Thermus thermophilus , including phages G20c, TSP4, P74-26, P23-45 and phiFA and the novel phage Tth15-6. Sequence and structural analysis of PolI_G20c revealed a 3′–5′ exonuclease domain and a DNA polymerase domain, and activity screening confirmed that both domains were functional. No functional 5′–3′ exonuclease domain was present. Structural analysis also revealed a novel specific structure motif, here termed SβαR, that was not previously identified in any polymerase belonging to the DNA polymerases I (or the DNA polymerase A family). The SβαR motif did not show any homology to the sequences or structures of known DNA polymerases. The exception was the sequence conservation of the residues in this motif in putative DNA polymerases encoded in the genomes of a group of thermophilic phages related to Thermus phage G20c. The structure of PolI_G20c was determined with the aid of another structure that was determined in parallel and was used as a model for molecular replacement. This other structure was of a 3′–5′ exonuclease termed ExnV1. The cloned and expressed gene encoding ExnV1 was isolated from a thermophilic virus metagenome that was collected from several hot springs in Iceland. The structure of ExnV1, which contains the novel SβαR motif, was first determined to 2.19 Å resolution. With these data at hand, the structure of PolI_G20c was determined to 2.97 Å resolution. The structures of PolI_G20c and ExnV1 are most similar to those of the Klenow fragment of DNA polymerase I (PDB entry 2kzz) from Escherichia coli , DNA polymerase I from Geobacillus stearothermophilus (PDB entry 1knc) and Taq polymerase (PDB entry 1bgx) from Thermus aquaticus ." @default.
• W4307407874 created "2022-11-01" @default.
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• W4307407874 date "2022-10-27" @default.
• W4307407874 modified "2023-10-14" @default.
• W4307407874 title "Crystal structure of DNA polymerase I from <i>Thermus</i> phage G20c" @default.
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• W4307407874 cites W1512345015 @default.
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• W4307407874 cites W1729887059 @default.
• W4307407874 cites W1892158199 @default.
• W4307407874 cites W1968740130 @default.
• W4307407874 cites W1973488583 @default.
• W4307407874 cites W1974554376 @default.
• W4307407874 cites W1979263207 @default.
• W4307407874 cites W1979511146 @default.
• W4307407874 cites W1980624564 @default.
• W4307407874 cites W1989252766 @default.
• W4307407874 cites W1995808589 @default.
• W4307407874 cites W2002118128 @default.
• W4307407874 cites W2003345264 @default.
• W4307407874 cites W2008954911 @default.
• W4307407874 cites W2017519756 @default.
• W4307407874 cites W2022382695 @default.
• W4307407874 cites W2023692235 @default.
• W4307407874 cites W2032796397 @default.
• W4307407874 cites W2035650458 @default.
• W4307407874 cites W2047013741 @default.
• W4307407874 cites W2058317622 @default.
• W4307407874 cites W2067371216 @default.
• W4307407874 cites W2068123384 @default.
• W4307407874 cites W2085866589 @default.
• W4307407874 cites W2096525273 @default.
• W4307407874 cites W2099723868 @default.
• W4307407874 cites W2110808180 @default.
• W4307407874 cites W2118392267 @default.
• W4307407874 cites W2120772351 @default.
• W4307407874 cites W2124026197 @default.
• W4307407874 cites W2127594598 @default.
• W4307407874 cites W2140673705 @default.
• W4307407874 cites W2143660875 @default.
• W4307407874 cites W2144998676 @default.
• W4307407874 cites W2149410625 @default.
• W4307407874 cites W2152778335 @default.
• W4307407874 cites W2153877213 @default.
• W4307407874 cites W2154545304 @default.
• W4307407874 cites W2159407190 @default.
• W4307407874 cites W2163341755 @default.
• W4307407874 cites W2164340098 @default.
• W4307407874 cites W2253430994 @default.
• W4307407874 cites W2557595285 @default.
• W4307407874 cites W2578349733 @default.
• W4307407874 cites W2623514069 @default.
• W4307407874 cites W2769990393 @default.
• W4307407874 cites W2799524357 @default.
• W4307407874 cites W2900142557 @default.
• W4307407874 cites W2924986168 @default.
• W4307407874 cites W29362848 @default.
• W4307407874 cites W2949020667 @default.
• W4307407874 cites W2951149542 @default.
• W4307407874 cites W2972411752 @default.
• W4307407874 cites W2980170804 @default.
• W4307407874 cites W2984894304 @default.
• W4307407874 cites W2991012415 @default.
• W4307407874 cites W3022960778 @default.
• W4307407874 cites W3094967361 @default.
• W4307407874 cites W3104756221 @default.
• W4307407874 cites W3137047737 @default.
• W4307407874 cites W3163782710 @default.
• W4307407874 cites W3166928849 @default.
• W4307407874 cites W3177828909 @default.
• W4307407874 cites W4226193853 @default.
• W4307407874 cites W4237445620 @default.
• W4307407874 cites W4241533829 @default.
• W4307407874 cites W4244736178 @default.
• W4307407874 cites W4247875838 @default.
• W4307407874 cites W4248872320 @default.
• W4307407874 cites W4251386234 @default.
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• W4307407874 doi "https://doi.org/10.1107/s2059798322009895" @default.
• W4307407874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36322421" @default.
• W4307407874 hasPublicationYear "2022" @default.
• W4307407874 type Work @default.

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