Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307445418> ?p ?o ?g. }
- W4307445418 abstract "Abstract Translational stalling events that result in ribosome collisions induce Ribosome-associated Quality Control (RQC) in order to degrade potentially toxic truncated nascent proteins. For RQC induction, the collided ribosomes are first marked by the Hel2/ZNF598 E3 ubiquitin ligase to recruit the RQT complex for subunit dissociation. In yeast, uS10 is polyubiquitinated by Hel2, whereas eS10 is preferentially monoubiquitinated by ZNF598 in human cells for an unknown reason. Here, we characterize the ubiquitination activity of ZNF598 and its importance for human RQT-mediated subunit dissociation using the endogenous XBP1u and poly(A) translation stallers. Cryo-EM analysis of a human collided disome reveals a distinct composite interface, with substantial differences to yeast collided disomes. Biochemical analysis of collided ribosomes shows that ZNF598 forms K63-linked polyubiquitin chains on uS10, which are decisive for mammalian RQC initiation. The human RQT (hRQT) complex composed only of ASCC3, ASCC2 and TRIP4 dissociates collided ribosomes dependent on the ATPase activity of ASCC3 and the ubiquitin-binding capacity of ASCC2. The hRQT-mediated subunit dissociation requires the K63-linked polyubiquitination of uS10, while monoubiquitination of eS10 or uS10 is not sufficient. Therefore, we conclude that ZNF598 functionally marks collided mammalian ribosomes by K63-linked polyubiquitination of uS10 for the trimeric hRQT complex-mediated subunit dissociation." @default.
- W4307445418 created "2022-11-01" @default.
- W4307445418 creator A5010441616 @default.
- W4307445418 creator A5010972091 @default.
- W4307445418 creator A5022355087 @default.
- W4307445418 creator A5023698098 @default.
- W4307445418 creator A5036019881 @default.
- W4307445418 creator A5041423546 @default.
- W4307445418 creator A5044309205 @default.
- W4307445418 creator A5057674861 @default.
- W4307445418 creator A5060702036 @default.
- W4307445418 creator A5061533640 @default.
- W4307445418 creator A5072883823 @default.
- W4307445418 creator A5084267967 @default.
- W4307445418 creator A5090352563 @default.
- W4307445418 date "2022-10-27" @default.
- W4307445418 modified "2023-10-14" @default.
- W4307445418 title "A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation" @default.
- W4307445418 cites W1835972708 @default.
- W4307445418 cites W1891202747 @default.
- W4307445418 cites W1968002307 @default.
- W4307445418 cites W2000789682 @default.
- W4307445418 cites W2018356588 @default.
- W4307445418 cites W2023374667 @default.
- W4307445418 cites W2039174905 @default.
- W4307445418 cites W2040614735 @default.
- W4307445418 cites W2056372900 @default.
- W4307445418 cites W2080872178 @default.
- W4307445418 cites W2097604487 @default.
- W4307445418 cites W2137355722 @default.
- W4307445418 cites W2180229411 @default.
- W4307445418 cites W2436623623 @default.
- W4307445418 cites W2568866823 @default.
- W4307445418 cites W2580905939 @default.
- W4307445418 cites W2587625522 @default.
- W4307445418 cites W2589978322 @default.
- W4307445418 cites W2593511418 @default.
- W4307445418 cites W2730564452 @default.
- W4307445418 cites W2734356283 @default.
- W4307445418 cites W2738059873 @default.
- W4307445418 cites W2895153371 @default.
- W4307445418 cites W2899714098 @default.
- W4307445418 cites W2908095537 @default.
- W4307445418 cites W2911351489 @default.
- W4307445418 cites W2922547250 @default.
- W4307445418 cites W2924025784 @default.
- W4307445418 cites W2924838299 @default.
- W4307445418 cites W2950967305 @default.
- W4307445418 cites W2955615660 @default.
- W4307445418 cites W2963893845 @default.
- W4307445418 cites W2969715383 @default.
- W4307445418 cites W2991309186 @default.
- W4307445418 cites W2995107466 @default.
- W4307445418 cites W3007950993 @default.
- W4307445418 cites W3010254892 @default.
- W4307445418 cites W3014995696 @default.
- W4307445418 cites W3035939632 @default.
- W4307445418 cites W3038768407 @default.
- W4307445418 cites W3041421192 @default.
- W4307445418 cites W3043335865 @default.
- W4307445418 cites W3043427328 @default.
- W4307445418 cites W3111841994 @default.
- W4307445418 cites W3148675655 @default.
- W4307445418 cites W3166388960 @default.
- W4307445418 cites W4220675341 @default.
- W4307445418 cites W4224209777 @default.
- W4307445418 cites W4245504988 @default.
- W4307445418 doi "https://doi.org/10.1038/s41467-022-34097-9" @default.
- W4307445418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36302773" @default.
- W4307445418 hasPublicationYear "2022" @default.
- W4307445418 type Work @default.
- W4307445418 citedByCount "16" @default.
- W4307445418 countsByYear W43074454182022 @default.
- W4307445418 countsByYear W43074454182023 @default.
- W4307445418 crossrefType "journal-article" @default.
- W4307445418 hasAuthorship W4307445418A5010441616 @default.
- W4307445418 hasAuthorship W4307445418A5010972091 @default.
- W4307445418 hasAuthorship W4307445418A5022355087 @default.
- W4307445418 hasAuthorship W4307445418A5023698098 @default.
- W4307445418 hasAuthorship W4307445418A5036019881 @default.
- W4307445418 hasAuthorship W4307445418A5041423546 @default.
- W4307445418 hasAuthorship W4307445418A5044309205 @default.
- W4307445418 hasAuthorship W4307445418A5057674861 @default.
- W4307445418 hasAuthorship W4307445418A5060702036 @default.
- W4307445418 hasAuthorship W4307445418A5061533640 @default.
- W4307445418 hasAuthorship W4307445418A5072883823 @default.
- W4307445418 hasAuthorship W4307445418A5084267967 @default.
- W4307445418 hasAuthorship W4307445418A5090352563 @default.
- W4307445418 hasBestOaLocation W43074454181 @default.
- W4307445418 hasConcept C104292427 @default.
- W4307445418 hasConcept C104317684 @default.
- W4307445418 hasConcept C134459356 @default.
- W4307445418 hasConcept C25602115 @default.
- W4307445418 hasConcept C55493867 @default.
- W4307445418 hasConcept C67705224 @default.
- W4307445418 hasConcept C86803240 @default.
- W4307445418 hasConcept C88478588 @default.
- W4307445418 hasConcept C95444343 @default.
- W4307445418 hasConceptScore W4307445418C104292427 @default.
- W4307445418 hasConceptScore W4307445418C104317684 @default.