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• W4307462428 abstract "Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used to prepare a thin lamella-shaped sample out of a frozen-hydrated cell for cryo-ET imaging, but standard cryo-FIB fabrication is blind to the precise location of the structure or proteins of interest. Fluorescence-guided focused ion beam (FIB) milling at target locations requires multiple sample transfers prone to contamination, and relocation and registration accuracy is often insufficient for 3D targeting. Here, we present in situ fluorescence microscopy-guided FIB fabrication of a frozen-hydrated lamella to address this problem: we built a coincident three-beam cryogenic correlative microscope by retrofitting a compact cryogenic microcooler, custom positioning stage, and an inverted widefield fluorescence microscope (FM) on an existing FIB scanning electron microscope. We show FM controlled targeting at every milling step in the lamella fabrication process, validated with transmission electron microscope tomogram reconstructions of the target regions. The ability to check the lamella during and after the milling process results in a higher success rate in the fabrication process and will increase the throughput of fabrication for lamellae suitable for high-resolution imaging." @default.
• W4307462428 created "2022-11-01" @default.
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• W4307462428 date "2022-10-28" @default.
• W4307462428 modified "2023-10-14" @default.
• W4307462428 title "A cryogenic, coincident fluorescence, electron, and ion beam microscope" @default.
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• W4307462428 doi "https://doi.org/10.7554/elife.82891" @default.
• W4307462428 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36305590" @default.
• W4307462428 hasPublicationYear "2022" @default.
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