Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307496419> ?p ?o ?g. }
- W4307496419 endingPage "438" @default.
- W4307496419 startingPage "427" @default.
- W4307496419 abstract "Pyroptosis, a form of cell death associated with inflammation, is known to be involved in diabetic nephropathy (DN), and discoid domain receptor 1 (DDR1), an inflammatory regulatory protein, is reported to be associated with diabetes. However, the mechanism underlying DDR1 regulation and pyroptosis in DN remains unknown. We aimed to investigate the effect of DDR1 on renal tubular epithelial cell pyroptosis and the mechanism underlying DN. In this study, we used high glucose (HG)-treated HK-2 cells and rats with a single intraperitoneal injection of streptozotocin as DN models. Subsequently, the expression of pyroptosis-related proteins (cleaved caspase-1, GSDMD-N, Interleukin-1β [IL-1β], and interleukin-18 [IL-18]), DDR1, phosphorylated NF-κB (p-NF-κB), and NLR family pyrin domain-containing 3 (NLRP3) inflammasomes were determined through Western blotting. IL-1β and IL-18 levels were determined using ELISA. The rate of pyroptosis was assessed by propidium iodide (PI) staining. The results revealed upregulated expression of pyroptosis-related proteins and increased concentration of IL-1β and IL-18, accompanied by DDR1, p-NF-κB, and NLRP3 upregulation in DN rat kidney tissues and HG-treated HK-2 cells. Moreover, DDR1 knockdown in the background of HG treatment resulted in inhibited expression of pyroptosis-related proteins and attenuation of IL-1β and IL-18 production and PI-positive cell frequency via the NF-κB/NLRP3 pathway in HK-2 cells. However, NLRP3 overexpression reversed the effect of DDR1 knockdown on pyroptosis. In conclusion, we demonstrated that DDR1 may be associated with pyroptosis, and DDR1 knockdown inhibited HG-induced renal tubular epithelial cell pyroptosis. The NF-κB/NLRP3 pathway is probably involved in the underlying mechanism of these findings." @default.
- W4307496419 created "2022-11-02" @default.
- W4307496419 creator A5007389190 @default.
- W4307496419 creator A5019084328 @default.
- W4307496419 creator A5041459683 @default.
- W4307496419 creator A5055166078 @default.
- W4307496419 creator A5057566317 @default.
- W4307496419 creator A5058126258 @default.
- W4307496419 creator A5079107379 @default.
- W4307496419 date "2022-11-01" @default.
- W4307496419 modified "2023-10-05" @default.
- W4307496419 title "The role of discoid domain receptor 1 on renal tubular epithelial pyroptosis in diabetic nephropathy" @default.
- W4307496419 cites W1971986557 @default.
- W4307496419 cites W1989532668 @default.
- W4307496419 cites W2026672268 @default.
- W4307496419 cites W2034986869 @default.
- W4307496419 cites W2058512463 @default.
- W4307496419 cites W2082636793 @default.
- W4307496419 cites W2101104783 @default.
- W4307496419 cites W2130886591 @default.
- W4307496419 cites W2165873691 @default.
- W4307496419 cites W2184894459 @default.
- W4307496419 cites W2287474094 @default.
- W4307496419 cites W2522054313 @default.
- W4307496419 cites W2585789979 @default.
- W4307496419 cites W2586758361 @default.
- W4307496419 cites W2626306527 @default.
- W4307496419 cites W2789997601 @default.
- W4307496419 cites W2790536030 @default.
- W4307496419 cites W2806500112 @default.
- W4307496419 cites W2809631602 @default.
- W4307496419 cites W2883687387 @default.
- W4307496419 cites W2893204356 @default.
- W4307496419 cites W2904055459 @default.
- W4307496419 cites W2928137362 @default.
- W4307496419 cites W2969750300 @default.
- W4307496419 cites W2970013466 @default.
- W4307496419 cites W2970702341 @default.
- W4307496419 cites W2987498568 @default.
- W4307496419 cites W2990249901 @default.
- W4307496419 cites W3014222798 @default.
- W4307496419 cites W3023190642 @default.
- W4307496419 cites W3027862107 @default.
- W4307496419 cites W3039374476 @default.
- W4307496419 cites W3044948032 @default.
- W4307496419 cites W3045902275 @default.
- W4307496419 cites W3091256280 @default.
- W4307496419 cites W3092090741 @default.
- W4307496419 cites W3102726452 @default.
- W4307496419 cites W3110045126 @default.
- W4307496419 cites W3125358760 @default.
- W4307496419 cites W3134176597 @default.
- W4307496419 cites W3135585463 @default.
- W4307496419 cites W3152675680 @default.
- W4307496419 cites W3154078168 @default.
- W4307496419 cites W3158874774 @default.
- W4307496419 cites W3176223843 @default.
- W4307496419 cites W3182728732 @default.
- W4307496419 cites W3186164714 @default.
- W4307496419 cites W3202902322 @default.
- W4307496419 cites W3203499024 @default.
- W4307496419 cites W3207366945 @default.
- W4307496419 cites W3209295792 @default.
- W4307496419 doi "https://doi.org/10.4196/kjpp.2022.26.6.427" @default.
- W4307496419 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36302618" @default.
- W4307496419 hasPublicationYear "2022" @default.
- W4307496419 type Work @default.
- W4307496419 citedByCount "1" @default.
- W4307496419 countsByYear W43074964192023 @default.
- W4307496419 crossrefType "journal-article" @default.
- W4307496419 hasAuthorship W4307496419A5007389190 @default.
- W4307496419 hasAuthorship W4307496419A5019084328 @default.
- W4307496419 hasAuthorship W4307496419A5041459683 @default.
- W4307496419 hasAuthorship W4307496419A5055166078 @default.
- W4307496419 hasAuthorship W4307496419A5057566317 @default.
- W4307496419 hasAuthorship W4307496419A5058126258 @default.
- W4307496419 hasAuthorship W4307496419A5079107379 @default.
- W4307496419 hasBestOaLocation W43074964191 @default.
- W4307496419 hasConcept C101544691 @default.
- W4307496419 hasConcept C104317684 @default.
- W4307496419 hasConcept C127561419 @default.
- W4307496419 hasConcept C139964883 @default.
- W4307496419 hasConcept C152681006 @default.
- W4307496419 hasConcept C162157009 @default.
- W4307496419 hasConcept C164027704 @default.
- W4307496419 hasConcept C170493617 @default.
- W4307496419 hasConcept C17172800 @default.
- W4307496419 hasConcept C173396325 @default.
- W4307496419 hasConcept C185592680 @default.
- W4307496419 hasConcept C190283241 @default.
- W4307496419 hasConcept C203014093 @default.
- W4307496419 hasConcept C2775934118 @default.
- W4307496419 hasConcept C2776914184 @default.
- W4307496419 hasConcept C2777209026 @default.
- W4307496419 hasConcept C31573885 @default.
- W4307496419 hasConcept C502942594 @default.
- W4307496419 hasConcept C55493867 @default.
- W4307496419 hasConcept C62478195 @default.