Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307608194> ?p ?o ?g. }
- W4307608194 abstract "Abstract In vitro investigations of host-pathogen interactions of viruses are reliant on suitable cell and tissue culture models. Results are only as good as the model they have been generated in. However, choosing cell models for in vitro work often depends on what is available in labs or what has previously been used. Despite the vast increase in coronavirus research activity over the past few years, researchers are still heavily reliant on: non-human cells, for example Vero E6, highly heterogeneous or not fully differentiated cells, such as Calu-3, or naturally unsusceptible cells requiring overexpression of receptors and other accessory factors, such as ACE2 and TMPRSS2. Complex cell models, such as primary cell-derived air-liquid interface epithelial models are highly representative of human tissues but are expensive and time-consuming to develop and maintain. They have limited suitability for large-scale and high-throughput analysis. Using tissue-specific expression pattern as a selection criteria, we identified human kidney cells as an ideal target for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and broader coronavirus infection. We show the use of the highly characterized human kidney cell line Caki-1 for infection with three human coronaviruses: Betacoronaviruses SARS-CoV-2 and Middle Eastern respiratory syndrome coronavirus (MERS-CoV) and Alphacoronavirus human coronavirus 229E (hCoV-229E). Caki-1 cells show equal or superior susceptibility to all three coronaviruses when compared to other commonly used cell lines for the cultivation of the respective virus. Antibody staining against SARS-CoV-2 N protein shows comparable replication rates. Using a panel of 21 antibodies in infected Caki-1 cells using immunocytochemistry shows the location of viral proteins during replication. In addition, Caki-1 cells were found to be susceptible to two other human respiratory viruses, influenza A virus and respiratory syncytial virus, making them an ideal model for cross-comparison of not only a broad range of coronaviruses but respiratory viruses in general. Author Summary Investigating how viruses interact with their host relies models used for laboratory research. The closer a model matches the host, the more conclusive results are. Complex cell systems based on primary epithelial or stem cells are the gold standard of in vitro research. However, they are expensive, time consuming, and laborious to establish. Therefore, cell lines remain the backbone of virus research. Despite vastly increased research into human coronaviruses following the COVID-19 pandemic, researchers continue to rely on suboptimal cell line models, for example ells of non-human origin like the VeroE6 African Green Monkey cell line. Using known expression patterns of the entry factors of the COVID-19 causative agent severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) we identified the Caki-1 cell line as a target for SARS-CoV-2. This cell line could be shown to be infectable with a wide range of coronaviruses including common cold virus hCoV-229E, epidemic virus MERS-CoV, and SARS-CoV-2 as well as other important respiratory viruses influenza A virus and respiratory syncytial virus. We could show that SARS-CoV-2 proteins can be stained for and localized in Caki-1 cells and the cells are competent of forming a cellular immune response. Together, this makes Caki-1 cells a unique tool for cross-virus comparison in one cell line." @default.
- W4307608194 created "2022-11-04" @default.
- W4307608194 creator A5006469947 @default.
- W4307608194 creator A5017211750 @default.
- W4307608194 creator A5031917567 @default.
- W4307608194 creator A5034921740 @default.
- W4307608194 creator A5039217955 @default.
- W4307608194 creator A5041829556 @default.
- W4307608194 creator A5056625467 @default.
- W4307608194 creator A5059029831 @default.
- W4307608194 creator A5081349326 @default.
- W4307608194 creator A5083113760 @default.
- W4307608194 creator A5086273776 @default.
- W4307608194 creator A5088084394 @default.
- W4307608194 date "2022-10-25" @default.
- W4307608194 modified "2023-09-27" @default.
- W4307608194 title "One for all – Human kidney Caki-1 cells are highly susceptible to infection with corona- and other respiratory viruses" @default.
- W4307608194 cites W1841998284 @default.
- W4307608194 cites W1972873370 @default.
- W4307608194 cites W1984628239 @default.
- W4307608194 cites W2005004164 @default.
- W4307608194 cites W2020172485 @default.
- W4307608194 cites W2025170735 @default.
- W4307608194 cites W2056873822 @default.
- W4307608194 cites W2060008923 @default.
- W4307608194 cites W2093392569 @default.
- W4307608194 cites W2111058723 @default.
- W4307608194 cites W2112825470 @default.
- W4307608194 cites W2114843025 @default.
- W4307608194 cites W2120015874 @default.
- W4307608194 cites W2121719851 @default.
- W4307608194 cites W2122511992 @default.
- W4307608194 cites W2130344921 @default.
- W4307608194 cites W2139682436 @default.
- W4307608194 cites W2143392704 @default.
- W4307608194 cites W2149441684 @default.
- W4307608194 cites W2150409982 @default.
- W4307608194 cites W2166867592 @default.
- W4307608194 cites W2330263046 @default.
- W4307608194 cites W2345542811 @default.
- W4307608194 cites W2768064447 @default.
- W4307608194 cites W3001971765 @default.
- W4307608194 cites W3016829967 @default.
- W4307608194 cites W3022285953 @default.
- W4307608194 cites W3027231799 @default.
- W4307608194 cites W3036391214 @default.
- W4307608194 cites W3082709602 @default.
- W4307608194 cites W3093900830 @default.
- W4307608194 cites W3094597057 @default.
- W4307608194 cites W3106737244 @default.
- W4307608194 cites W3110976643 @default.
- W4307608194 cites W3128335178 @default.
- W4307608194 cites W3132092747 @default.
- W4307608194 cites W3134338237 @default.
- W4307608194 cites W3137886193 @default.
- W4307608194 cites W3152451364 @default.
- W4307608194 cites W3158021769 @default.
- W4307608194 cites W3159182925 @default.
- W4307608194 cites W3159350301 @default.
- W4307608194 cites W3181937414 @default.
- W4307608194 cites W3193102476 @default.
- W4307608194 cites W4210665241 @default.
- W4307608194 cites W4211100855 @default.
- W4307608194 cites W4214757808 @default.
- W4307608194 cites W4224862904 @default.
- W4307608194 cites W4225699259 @default.
- W4307608194 cites W4226147054 @default.
- W4307608194 cites W4280575755 @default.
- W4307608194 cites W4283741483 @default.
- W4307608194 doi "https://doi.org/10.1101/2022.10.25.513760" @default.
- W4307608194 hasPublicationYear "2022" @default.
- W4307608194 type Work @default.
- W4307608194 citedByCount "0" @default.
- W4307608194 crossrefType "posted-content" @default.
- W4307608194 hasAuthorship W4307608194A5006469947 @default.
- W4307608194 hasAuthorship W4307608194A5017211750 @default.
- W4307608194 hasAuthorship W4307608194A5031917567 @default.
- W4307608194 hasAuthorship W4307608194A5034921740 @default.
- W4307608194 hasAuthorship W4307608194A5039217955 @default.
- W4307608194 hasAuthorship W4307608194A5041829556 @default.
- W4307608194 hasAuthorship W4307608194A5056625467 @default.
- W4307608194 hasAuthorship W4307608194A5059029831 @default.
- W4307608194 hasAuthorship W4307608194A5081349326 @default.
- W4307608194 hasAuthorship W4307608194A5083113760 @default.
- W4307608194 hasAuthorship W4307608194A5086273776 @default.
- W4307608194 hasAuthorship W4307608194A5088084394 @default.
- W4307608194 hasBestOaLocation W43076081941 @default.
- W4307608194 hasConcept C134018914 @default.
- W4307608194 hasConcept C142724271 @default.
- W4307608194 hasConcept C1491633281 @default.
- W4307608194 hasConcept C159047783 @default.
- W4307608194 hasConcept C159654299 @default.
- W4307608194 hasConcept C174510640 @default.
- W4307608194 hasConcept C203014093 @default.
- W4307608194 hasConcept C2522874641 @default.
- W4307608194 hasConcept C2776525042 @default.
- W4307608194 hasConcept C2777648638 @default.
- W4307608194 hasConcept C2779134260 @default.
- W4307608194 hasConcept C3008058167 @default.
- W4307608194 hasConcept C35866371 @default.