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- W4307730326 abstract "Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated.The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains." @default.
- W4307730326 created "2022-11-05" @default.
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- W4307730326 date "2022-10-29" @default.
- W4307730326 modified "2023-09-30" @default.
- W4307730326 title "CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis" @default.
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- W4307730326 doi "https://doi.org/10.1007/978-1-0716-2795-2_15" @default.
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