FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307804570> ?p ?o ?g. }

• W4307804570 abstract "Gene context can have significant impact on gene expression but is currently not integrated in quantitative models of gene regulation despite known biophysical principles and quantitative in vitro measurements. Conceptually, the simplest gene context consists of a single gene framed by two topological barriers, known as the twin transcriptional-loop model, which illustrates the interplay between transcription and DNA supercoiling. In vivo , DNA supercoiling is additionally modulated by topoisomerases, whose modus operandi remains to be quantified. Here, we bridge the gap between theory and in vivo properties by realizing in Escherichia coli the twin transcriptional-loop model and by measuring how gene expression varies with promoters and distances to the topological barriers. We find that gene expression depends on the distance to the upstream barrier but not to the downstream barrier, with a promoter-dependent intensity. We rationalize these findings with a first-principle biophysical model of DNA transcription. The model integrates binding, initiation and elongation of RNA polymerases parametrized with available in vitro measurements, as well as the action of topoisomerases for which parameters are constrained by our experimental results. By comparing it with the data, our biophysical model supports that TopoI and gyrase must both act specifically, respectively upstream and downstream the gene, and predicts TopoI to be less active than gyrase. It also highlights antagonistic effects of TopoI, which both facilitates elongation and tends to repress initiation. Altogether, our work sets the foundations for a systematic and quantitative description of the impact of gene context on gene regulation." @default.
• W4307804570 created "2022-11-06" @default.
• W4307804570 creator A5018388946 @default.
• W4307804570 creator A5047786562 @default.
• W4307804570 creator A5058947652 @default.
• W4307804570 creator A5068503267 @default.
• W4307804570 creator A5078626534 @default.
• W4307804570 date "2022-11-01" @default.
• W4307804570 modified "2023-10-18" @default.
• W4307804570 title "Assessing<i>in vivo</i>the impact of gene context on transcription through DNA supercoiling" @default.
• W4307804570 cites W1543999940 @default.
• W4307804570 cites W1551727602 @default.
• W4307804570 cites W1968698890 @default.
• W4307804570 cites W1972168116 @default.
• W4307804570 cites W1974470327 @default.
• W4307804570 cites W1986761529 @default.
• W4307804570 cites W2001987130 @default.
• W4307804570 cites W2003470745 @default.
• W4307804570 cites W2005506831 @default.
• W4307804570 cites W2018349754 @default.
• W4307804570 cites W2033565485 @default.
• W4307804570 cites W2046892161 @default.
• W4307804570 cites W2053783798 @default.
• W4307804570 cites W2055506322 @default.
• W4307804570 cites W2058940094 @default.
• W4307804570 cites W2069211652 @default.
• W4307804570 cites W2075743405 @default.
• W4307804570 cites W2078635599 @default.
• W4307804570 cites W2093777918 @default.
• W4307804570 cites W2097205369 @default.
• W4307804570 cites W2097697790 @default.
• W4307804570 cites W2101058845 @default.
• W4307804570 cites W2107282968 @default.
• W4307804570 cites W2109778555 @default.
• W4307804570 cites W2137088684 @default.
• W4307804570 cites W2140158396 @default.
• W4307804570 cites W2140401788 @default.
• W4307804570 cites W2156425900 @default.
• W4307804570 cites W2158208080 @default.
• W4307804570 cites W2168990146 @default.
• W4307804570 cites W2327011266 @default.
• W4307804570 cites W2407073328 @default.
• W4307804570 cites W2407486174 @default.
• W4307804570 cites W2461066986 @default.
• W4307804570 cites W2516060948 @default.
• W4307804570 cites W2611717162 @default.
• W4307804570 cites W2728309241 @default.
• W4307804570 cites W2740036970 @default.
• W4307804570 cites W2769190237 @default.
• W4307804570 cites W2769580812 @default.
• W4307804570 cites W2777639606 @default.
• W4307804570 cites W2910205094 @default.
• W4307804570 cites W2952097502 @default.
• W4307804570 cites W2963402579 @default.
• W4307804570 cites W2974890644 @default.
• W4307804570 cites W3048875949 @default.
• W4307804570 cites W3133621079 @default.
• W4307804570 cites W3157858663 @default.
• W4307804570 cites W4206589516 @default.
• W4307804570 cites W4239608022 @default.
• W4307804570 cites W4289837680 @default.
• W4307804570 doi "https://doi.org/10.1101/2022.10.31.514473" @default.
• W4307804570 hasPublicationYear "2022" @default.
• W4307804570 type Work @default.
• W4307804570 citedByCount "0" @default.
• W4307804570 crossrefType "posted-content" @default.
• W4307804570 hasAuthorship W4307804570A5018388946 @default.
• W4307804570 hasAuthorship W4307804570A5047786562 @default.
• W4307804570 hasAuthorship W4307804570A5058947652 @default.
• W4307804570 hasAuthorship W4307804570A5068503267 @default.
• W4307804570 hasAuthorship W4307804570A5078626534 @default.
• W4307804570 hasBestOaLocation W43078045701 @default.
• W4307804570 hasConcept C104317684 @default.
• W4307804570 hasConcept C138885662 @default.
• W4307804570 hasConcept C147897179 @default.
• W4307804570 hasConcept C150194340 @default.
• W4307804570 hasConcept C151730666 @default.
• W4307804570 hasConcept C158456008 @default.
• W4307804570 hasConcept C165864922 @default.
• W4307804570 hasConcept C167125095 @default.
• W4307804570 hasConcept C179926584 @default.
• W4307804570 hasConcept C27153228 @default.
• W4307804570 hasConcept C2779343474 @default.
• W4307804570 hasConcept C41895202 @default.
• W4307804570 hasConcept C54355233 @default.
• W4307804570 hasConcept C547475151 @default.
• W4307804570 hasConcept C552990157 @default.
• W4307804570 hasConcept C70721500 @default.
• W4307804570 hasConcept C73573662 @default.
• W4307804570 hasConcept C86803240 @default.
• W4307804570 hasConceptScore W4307804570C104317684 @default.
• W4307804570 hasConceptScore W4307804570C138885662 @default.
• W4307804570 hasConceptScore W4307804570C147897179 @default.
• W4307804570 hasConceptScore W4307804570C150194340 @default.
• W4307804570 hasConceptScore W4307804570C151730666 @default.
• W4307804570 hasConceptScore W4307804570C158456008 @default.
• W4307804570 hasConceptScore W4307804570C165864922 @default.
• W4307804570 hasConceptScore W4307804570C167125095 @default.
• W4307804570 hasConceptScore W4307804570C179926584 @default.
• W4307804570 hasConceptScore W4307804570C27153228 @default.

• next