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- W4307831216 abstract "Abstract Deconvolution is a challenging inverse problem, particularly in techniques that employ complex engineered point-spread functions, such as microscopy with propagation-invariant beams. Here, we present a deep-learning method for deconvolution that, in lieu of end-to-end training with ground truths, is trained using known physics of the imaging system. Specifically, we train a generative adversarial network with images generated with the known point-spread function of the system, and combine this with unpaired experimental data that preserve perceptual content. Our method rapidly and robustly deconvolves and super-resolves microscopy images, demonstrating a two-fold improvement in image contrast to conventional deconvolution methods. In contrast to common end-to-end networks that often require 1000–10,000s paired images, our method is experimentally unsupervised and can be trained solely on a few hundred regions of interest. We demonstrate its performance on light-sheet microscopy with propagation-invariant Airy beams in oocytes, preimplantation embryos and excised brain tissue, as well as illustrate its utility for Bessel-beam LSM. This method aims to democratise learned methods for deconvolution, as it does not require data acquisition outwith the conventional imaging protocol." @default.
- W4307831216 created "2022-11-06" @default.
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- W4307831216 date "2022-11-02" @default.
- W4307831216 modified "2023-10-14" @default.
- W4307831216 title "Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams" @default.
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- W4307831216 doi "https://doi.org/10.1038/s41377-022-00975-6" @default.
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- W4307831216 hasPublicationYear "2022" @default.
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