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- W4307922277 endingPage "340576" @default.
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- W4307922277 abstract "Listeria monocytogenes (L. monocytogenes), a typical foodborne pathogen, poses a serious threat to public health safety. This stimulates to develop a point-of-care testing (POCT) method to achieve rapid, sensitive detection of L. monocytogenes. In this study, polyethylene glycol (PEG) mediated ampicillin functionalized magnetic beads (Amp-PEG-MBs) was prepared successfully and it achieved high efficiency (>90%) and rapid (5 min) capture for L. monocytogenes at room temperature. The innovative combination of antibody (Ab), glucose oxidase (GOD) and graphene oxide (GO) prepared [email protected]@GOD for the specific recognition of L. monocytogenes. Finally, Amp-PEG-MBs and [email protected]@GOD were successfully assembled into [email protected]L. monocytogenes[email protected]@GOD sandwich structure which could catalyze the glucose, and the final detection results were recorded by a blood glucose meter (BGM). Magnetic separation (MS) combined with enzyme-catalyzed sensor ([email protected]@GOD-BGM) was successfully established to achieve the detection of L. monocytogenes in artificially contaminated juice within 66 min with the limit of detection was 101 CFU/mL. This sensor has potential for other pathogens detection by modifying specific antibodies." @default.
- W4307922277 created "2022-11-06" @default.
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- W4307922277 date "2022-12-01" @default.
- W4307922277 modified "2023-09-26" @default.
- W4307922277 title "Portable sensor based on magnetic separation and enzyme-mediated immune nanomaterials for point-of-care testing of Listeria monocytogenes in food" @default.
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- W4307922277 doi "https://doi.org/10.1016/j.aca.2022.340576" @default.
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