Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307940186> ?p ?o ?g. }
- W4307940186 endingPage "585" @default.
- W4307940186 startingPage "585" @default.
- W4307940186 abstract "Replicating plasmid shuttle vectors are key tools for efficient genetic and metabolic engineering applications, allowing the development of sustainable bioprocesses using non-model organisms with unique metabolic capabilities. To date, very limited genetic manipulation has been achieved in the thermophilic acetogen, Moorella thermoacetica, partly due to the lack of suitable shuttle vectors. However, M. thermoacetica has considerable potential as an industrial chassis organism, which can only be unlocked if reliable and effective genetic tools are in place. This study reports the construction of a replicating shuttle vector for M. thermoacetica through the identification and implementation of a compatible Gram-positive replicon to allow plasmid maintenance within the host. Although characterisation of plasmid behaviour proved difficult, the designed shuttle vector was subsequently applied for ethanologenesis, i.e., ethanol production in this organism. The non-native ethanologenesis in M. thermoacetica was achieved via plasmid-borne overexpression of the native aldh gene and heterologous expression of Clostridium autoethanogenum adhE1 gene. This result demonstrates the importance of the developed replicating plasmid vector for genetic and metabolic engineering efforts in industrially important M. thermoacetica." @default.
- W4307940186 created "2022-11-06" @default.
- W4307940186 creator A5034953908 @default.
- W4307940186 creator A5042215252 @default.
- W4307940186 creator A5044949409 @default.
- W4307940186 creator A5053248552 @default.
- W4307940186 creator A5089399252 @default.
- W4307940186 date "2022-10-28" @default.
- W4307940186 modified "2023-09-30" @default.
- W4307940186 title "Enabling Ethanologenesis in Moorella thermoacetica through Construction of a Replicating Shuttle Vector" @default.
- W4307940186 cites W1570745070 @default.
- W4307940186 cites W1968305507 @default.
- W4307940186 cites W1980696141 @default.
- W4307940186 cites W1999062010 @default.
- W4307940186 cites W2022744472 @default.
- W4307940186 cites W2037029203 @default.
- W4307940186 cites W2047821263 @default.
- W4307940186 cites W2051612190 @default.
- W4307940186 cites W2058116338 @default.
- W4307940186 cites W2069876236 @default.
- W4307940186 cites W2070196801 @default.
- W4307940186 cites W2077787127 @default.
- W4307940186 cites W2078464013 @default.
- W4307940186 cites W2079628735 @default.
- W4307940186 cites W2084558677 @default.
- W4307940186 cites W2092461073 @default.
- W4307940186 cites W2107277218 @default.
- W4307940186 cites W2111420829 @default.
- W4307940186 cites W2118473805 @default.
- W4307940186 cites W2121197506 @default.
- W4307940186 cites W2128898932 @default.
- W4307940186 cites W2141836311 @default.
- W4307940186 cites W2142338791 @default.
- W4307940186 cites W2156738105 @default.
- W4307940186 cites W2160084442 @default.
- W4307940186 cites W2169744530 @default.
- W4307940186 cites W2294276789 @default.
- W4307940186 cites W2327442225 @default.
- W4307940186 cites W2329589458 @default.
- W4307940186 cites W2413011000 @default.
- W4307940186 cites W2538295181 @default.
- W4307940186 cites W2547891072 @default.
- W4307940186 cites W2564573604 @default.
- W4307940186 cites W2573626724 @default.
- W4307940186 cites W2579861241 @default.
- W4307940186 cites W2619521384 @default.
- W4307940186 cites W2765466553 @default.
- W4307940186 cites W2769316169 @default.
- W4307940186 cites W2808310382 @default.
- W4307940186 cites W2910544292 @default.
- W4307940186 cites W2926688165 @default.
- W4307940186 cites W2932929973 @default.
- W4307940186 cites W2935872382 @default.
- W4307940186 cites W2965106244 @default.
- W4307940186 cites W2991713984 @default.
- W4307940186 cites W3004140701 @default.
- W4307940186 cites W3132794274 @default.
- W4307940186 cites W3156852721 @default.
- W4307940186 cites W4247447075 @default.
- W4307940186 cites W4281621948 @default.
- W4307940186 cites W802740667 @default.
- W4307940186 doi "https://doi.org/10.3390/fermentation8110585" @default.
- W4307940186 hasPublicationYear "2022" @default.
- W4307940186 type Work @default.
- W4307940186 citedByCount "2" @default.
- W4307940186 countsByYear W43079401862023 @default.
- W4307940186 crossrefType "journal-article" @default.
- W4307940186 hasAuthorship W4307940186A5034953908 @default.
- W4307940186 hasAuthorship W4307940186A5042215252 @default.
- W4307940186 hasAuthorship W4307940186A5044949409 @default.
- W4307940186 hasAuthorship W4307940186A5053248552 @default.
- W4307940186 hasAuthorship W4307940186A5089399252 @default.
- W4307940186 hasBestOaLocation W43079401861 @default.
- W4307940186 hasConcept C104317684 @default.
- W4307940186 hasConcept C145640855 @default.
- W4307940186 hasConcept C18103114 @default.
- W4307940186 hasConcept C22744801 @default.
- W4307940186 hasConcept C40767141 @default.
- W4307940186 hasConcept C54355233 @default.
- W4307940186 hasConcept C70721500 @default.
- W4307940186 hasConcept C86803240 @default.
- W4307940186 hasConcept C92087593 @default.
- W4307940186 hasConceptScore W4307940186C104317684 @default.
- W4307940186 hasConceptScore W4307940186C145640855 @default.
- W4307940186 hasConceptScore W4307940186C18103114 @default.
- W4307940186 hasConceptScore W4307940186C22744801 @default.
- W4307940186 hasConceptScore W4307940186C40767141 @default.
- W4307940186 hasConceptScore W4307940186C54355233 @default.
- W4307940186 hasConceptScore W4307940186C70721500 @default.
- W4307940186 hasConceptScore W4307940186C86803240 @default.
- W4307940186 hasConceptScore W4307940186C92087593 @default.
- W4307940186 hasIssue "11" @default.
- W4307940186 hasLocation W43079401861 @default.
- W4307940186 hasLocation W43079401862 @default.
- W4307940186 hasLocation W43079401863 @default.
- W4307940186 hasOpenAccess W4307940186 @default.
- W4307940186 hasPrimaryLocation W43079401861 @default.
- W4307940186 hasRelatedWork W1975180912 @default.