FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307967941> ?p ?o ?g. }

• W4307967941 endingPage "2686" @default.
• W4307967941 startingPage "2672" @default.
• W4307967941 abstract "Bacterial chemoreceptors regulate the cytosolic multidomain histidine kinase CheA through largely unknown mechanisms. Residue substitutions in the peptide linkers that connect the P4 kinase domain to the P3 dimerization and P5 regulatory domain affect CheA basal activity and activation. To understand the role that these linkers play in CheA activity, the P3-to-P4 linker (L3) and P4-to-P5 linker (L4) were extended and altered in variants of Thermotoga maritima (Tm) CheA. Flexible extensions of the L3 and L4 linkers in CheA-LV1 (linker variant 1) allowed for a well-folded kinase domain that retained wild-type (WT)-like binding affinities for nucleotide and normal interactions with the receptor-coupling protein CheW. However, CheA-LV1 autophosphorylation activity registered ∼50-fold lower compared to WT. Neither a WT nor LV1 dimer containing a single P4 domain could autophosphorylate the P1 substrate domain. Autophosphorylation activity was rescued in variants with extended L3 and L4 linkers that favor helical structure and heptad spacing. Autophosphorylation depended on linker spacing and flexibility and not on sequence. Pulse-dipolar electron-spin resonance (ESR) measurements with spin-labeled adenosine 5'-triphosphate (ATP) analogues indicated that CheA autophosphorylation activity inversely correlated with the proximity of the P4 domains within the dimers of the variants. Despite their separation in primary sequence and space, the L3 and L4 linkers also influence the mobility of the P1 substrate domains. In all, interactions of the P4 domains, as modulated by the L3 and L4 linkers, affect domain dynamics and autophosphorylation of CheA, thereby providing potential mechanisms for receptors to regulate the kinase." @default.
• W4307967941 created "2022-11-06" @default.
• W4307967941 creator A5034108175 @default.
• W4307967941 creator A5038717852 @default.
• W4307967941 creator A5071526287 @default.
• W4307967941 creator A5085492763 @default.
• W4307967941 date "2022-11-02" @default.
• W4307967941 modified "2023-10-15" @default.
• W4307967941 title "Interdomain Linkers Regulate Histidine Kinase Activity by Controlling Subunit Interactions" @default.
• W4307967941 cites W1593075457 @default.
• W4307967941 cites W1724277458 @default.
• W4307967941 cites W1957729318 @default.
• W4307967941 cites W1966405432 @default.
• W4307967941 cites W1966944263 @default.
• W4307967941 cites W1967147175 @default.
• W4307967941 cites W1972472647 @default.
• W4307967941 cites W1975769829 @default.
• W4307967941 cites W1981418540 @default.
• W4307967941 cites W1981877126 @default.
• W4307967941 cites W1984084102 @default.
• W4307967941 cites W1987026072 @default.
• W4307967941 cites W1991149372 @default.
• W4307967941 cites W1991200168 @default.
• W4307967941 cites W1992461112 @default.
• W4307967941 cites W1998423394 @default.
• W4307967941 cites W1999648327 @default.
• W4307967941 cites W2000443388 @default.
• W4307967941 cites W2005952337 @default.
• W4307967941 cites W2007804411 @default.
• W4307967941 cites W2008630890 @default.
• W4307967941 cites W2009900253 @default.
• W4307967941 cites W2010742248 @default.
• W4307967941 cites W2012778514 @default.
• W4307967941 cites W2017139960 @default.
• W4307967941 cites W2017785940 @default.
• W4307967941 cites W2018816634 @default.
• W4307967941 cites W2034300542 @default.
• W4307967941 cites W2035991134 @default.
• W4307967941 cites W2041728208 @default.
• W4307967941 cites W2043284163 @default.
• W4307967941 cites W2048205348 @default.
• W4307967941 cites W2054735066 @default.
• W4307967941 cites W2063002703 @default.
• W4307967941 cites W2064390126 @default.
• W4307967941 cites W2064441783 @default.
• W4307967941 cites W2073634930 @default.
• W4307967941 cites W2074279517 @default.
• W4307967941 cites W2093755926 @default.
• W4307967941 cites W2096515244 @default.
• W4307967941 cites W2098027198 @default.
• W4307967941 cites W2106144388 @default.
• W4307967941 cites W2108949591 @default.
• W4307967941 cites W2116056203 @default.
• W4307967941 cites W2124983865 @default.
• W4307967941 cites W2136148509 @default.
• W4307967941 cites W2137513214 @default.
• W4307967941 cites W2142654956 @default.
• W4307967941 cites W2149500199 @default.
• W4307967941 cites W2155142409 @default.
• W4307967941 cites W2173264508 @default.
• W4307967941 cites W223041692 @default.
• W4307967941 cites W2346627216 @default.
• W4307967941 cites W2592099752 @default.
• W4307967941 cites W2607429622 @default.
• W4307967941 cites W2753348948 @default.
• W4307967941 cites W2767194403 @default.
• W4307967941 cites W2788131605 @default.
• W4307967941 cites W2792017811 @default.
• W4307967941 cites W2810784684 @default.
• W4307967941 cites W2891330852 @default.
• W4307967941 cites W2901307736 @default.
• W4307967941 cites W2966382139 @default.
• W4307967941 cites W2971856795 @default.
• W4307967941 cites W2998439941 @default.
• W4307967941 cites W3018428875 @default.
• W4307967941 cites W3103273573 @default.
• W4307967941 cites W3111758673 @default.
• W4307967941 cites W4206999464 @default.
• W4307967941 doi "https://doi.org/10.1021/acs.biochem.2c00326" @default.
• W4307967941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36321948" @default.
• W4307967941 hasPublicationYear "2022" @default.
• W4307967941 type Work @default.
• W4307967941 citedByCount "2" @default.
• W4307967941 countsByYear W43079679412023 @default.
• W4307967941 crossrefType "journal-article" @default.
• W4307967941 hasAuthorship W4307967941A5034108175 @default.
• W4307967941 hasAuthorship W4307967941A5038717852 @default.
• W4307967941 hasAuthorship W4307967941A5071526287 @default.
• W4307967941 hasAuthorship W4307967941A5085492763 @default.
• W4307967941 hasConcept C104317684 @default.
• W4307967941 hasConcept C111919701 @default.
• W4307967941 hasConcept C12554922 @default.
• W4307967941 hasConcept C143065580 @default.
• W4307967941 hasConcept C150109051 @default.
• W4307967941 hasConcept C166342909 @default.
• W4307967941 hasConcept C181199279 @default.
• W4307967941 hasConcept C184235292 @default.
• W4307967941 hasConcept C185592680 @default.

• next