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• W4308005206 abstract "Chronic kidney disease (CKD) is a serious disease that endangers human health. It is reported that inhibiting renal cell apoptosis can delay the progress of CKD. Our previous study found that the mice with protein phosphatase Mg2+/Mn2+ dependent 1K (PPM1K) gene deletion had obvious symptoms of glomerular vascular and interstitial vascular dilatation, congestion and hemorrhage, glomerular hemorrhage and necrosis, interstitial fibrous tissue proliferation, decreased urinary creatinine clearance, and increased urinary protein level. In addition, studies have found that PPM1K is essential for cell survival, apoptosis and metabolism. However, no study has confirmed that PPM1K can inhibit renal cell apoptosis. In this study, PPM1K was overexpressed in human kidney-2 cells (HK-2), and the biological process of differentially expressed genes and its effect on apoptosis were comprehensively screened by RNA sequencing (RNA-seq). Through sequencing analysis, we found that there were 796 differentially expressed genes in human renal tubular epithelial cells transfected with PPM1K gene, of which 553 were down-regulated and 243 were up-regulated. Enrichment analysis found that differentially expressed genes may play an important role in amino acid metabolism and biosynthesis. In the GO analysis functional pathway list, we also found that multiple genes can be enriched in apoptosis related pathways, such as G0S2, GADD45A, TRIB3, VEGFA, NUPR1 and other up-regulated genes, and IL-6, MAGED1, CCL2, TP53INP1 and other down-regulated genes. Then we verified these differentially expressed genes by RT-PCR, and found that only the RT-PCR results of G0S2, VEGFA and NUPR1 were consistent with the transcriptome sequencing results. We believe that G0S2, VEGFA, NUPR1 and other genes may participate in the apoptosis process of HK-2 cells induced by PPM1K.In conclusion, these findings provide some data support for the study of HK-2 cell apoptosis mechanism, and also provide a scientific theoretical basis for further study of the effect of PPM1K on kidney disease." @default.
• W4308005206 created "2022-11-07" @default.
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• W4308005206 date "2022-11-01" @default.
• W4308005206 modified "2023-09-30" @default.
• W4308005206 title "The expression of apoptosis related genes in HK-2 cells overexpressing PPM1K was determined by RNA-seq analysis" @default.
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• W4308005206 cites W1974725392 @default.
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• W4308005206 cites W2000201704 @default.
• W4308005206 cites W2002010679 @default.
• W4308005206 cites W2005537109 @default.
• W4308005206 cites W2008757142 @default.
• W4308005206 cites W2012992125 @default.
• W4308005206 cites W2020317791 @default.
• W4308005206 cites W2027847170 @default.
• W4308005206 cites W2048075418 @default.
• W4308005206 cites W2053881507 @default.
• W4308005206 cites W2054865582 @default.
• W4308005206 cites W2061603378 @default.
• W4308005206 cites W2066471150 @default.
• W4308005206 cites W2072157707 @default.
• W4308005206 cites W2076254572 @default.
• W4308005206 cites W2080068472 @default.
• W4308005206 cites W2084284022 @default.
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• W4308005206 cites W2135546199 @default.
• W4308005206 cites W2136729555 @default.
• W4308005206 cites W2140298033 @default.
• W4308005206 cites W2141458291 @default.
• W4308005206 cites W2142237411 @default.
• W4308005206 cites W2154423739 @default.
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• W4308005206 cites W2162289930 @default.
• W4308005206 cites W2179438025 @default.
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• W4308005206 cites W2336289717 @default.
• W4308005206 cites W2337136207 @default.
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• W4308005206 doi "https://doi.org/10.3389/fgene.2022.1004610" @default.
• W4308005206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36386814" @default.
• W4308005206 hasPublicationYear "2022" @default.
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