Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308011662> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W4308011662 abstract "Amyloid fibril formation is involved in various human diseases, such as amyotrophic lateral sclerosis and Parkinson’s disease. Many amyloidogenic proteins undergo liquid–liquid phase separation (LLPS), forming liquid-like droplets of condensed proteins. Although these droplets represent potential amyloid nucleation sites, the kinetics of amyloid nucleation in droplets is poorly understood owing to the lack of quantitative analytical methods. This study aimed to develop a measurement method for the determination of amyloid droplet nucleation rate based on image analysis. Here, the prion determinant domain of the yeast prion protein Sup35 (Sup35NM), which is known as a model protein of prion diseases, was used for demonstration. Droplets (1–20 µm in size) of Sup35NM were fixed in agarose gels, and amyloid nucleation in each droplet was counted via confocal microscopy. From the comparison of the amyloid nucleation rates in the droplets and aqueous solutions, we found a critical concentration (C*) of Sup35NM: amyloid nucleation is suppressed by LLPS above C*, whereas it is enhanced below C*. These results highlight that droplet formation can suppress amyloid nucleation, contrary to current hypotheses in which the amyloid nucleation is enhanced by droplet formation. The proposed quantitative measurement method can provide accurate assessment of the role of LLPS for amyloidosis-related proteins from pathological perspectives." @default.
- W4308011662 created "2022-11-07" @default.
- W4308011662 creator A5002532344 @default.
- W4308011662 creator A5020449827 @default.
- W4308011662 creator A5040951977 @default.
- W4308011662 creator A5044258197 @default.
- W4308011662 creator A5059976144 @default.
- W4308011662 creator A5062976068 @default.
- W4308011662 creator A5063441153 @default.
- W4308011662 creator A5067888768 @default.
- W4308011662 creator A5068223676 @default.
- W4308011662 creator A5089656647 @default.
- W4308011662 creator A5073654101 @default.
- W4308011662 date "2022-11-01" @default.
- W4308011662 modified "2023-09-27" @default.
- W4308011662 title "Kinetic quantitative analysis reveals the suppression of Sup35NM amyloid fibril nucleation by liquid-liquid phase separation." @default.
- W4308011662 doi "https://doi.org/10.26434/chemrxiv-2022-tp1cm" @default.
- W4308011662 hasPublicationYear "2022" @default.
- W4308011662 type Work @default.
- W4308011662 citedByCount "0" @default.
- W4308011662 crossrefType "posted-content" @default.
- W4308011662 hasAuthorship W4308011662A5002532344 @default.
- W4308011662 hasAuthorship W4308011662A5020449827 @default.
- W4308011662 hasAuthorship W4308011662A5040951977 @default.
- W4308011662 hasAuthorship W4308011662A5044258197 @default.
- W4308011662 hasAuthorship W4308011662A5059976144 @default.
- W4308011662 hasAuthorship W4308011662A5062976068 @default.
- W4308011662 hasAuthorship W4308011662A5063441153 @default.
- W4308011662 hasAuthorship W4308011662A5067888768 @default.
- W4308011662 hasAuthorship W4308011662A5068223676 @default.
- W4308011662 hasAuthorship W4308011662A5073654101 @default.
- W4308011662 hasAuthorship W4308011662A5089656647 @default.
- W4308011662 hasBestOaLocation W43080116621 @default.
- W4308011662 hasConcept C121332964 @default.
- W4308011662 hasConcept C12554922 @default.
- W4308011662 hasConcept C136238340 @default.
- W4308011662 hasConcept C148898269 @default.
- W4308011662 hasConcept C178790620 @default.
- W4308011662 hasConcept C179104552 @default.
- W4308011662 hasConcept C185592680 @default.
- W4308011662 hasConcept C27523624 @default.
- W4308011662 hasConcept C2777633098 @default.
- W4308011662 hasConcept C55493867 @default.
- W4308011662 hasConcept C61048295 @default.
- W4308011662 hasConcept C62520636 @default.
- W4308011662 hasConcept C86803240 @default.
- W4308011662 hasConceptScore W4308011662C121332964 @default.
- W4308011662 hasConceptScore W4308011662C12554922 @default.
- W4308011662 hasConceptScore W4308011662C136238340 @default.
- W4308011662 hasConceptScore W4308011662C148898269 @default.
- W4308011662 hasConceptScore W4308011662C178790620 @default.
- W4308011662 hasConceptScore W4308011662C179104552 @default.
- W4308011662 hasConceptScore W4308011662C185592680 @default.
- W4308011662 hasConceptScore W4308011662C27523624 @default.
- W4308011662 hasConceptScore W4308011662C2777633098 @default.
- W4308011662 hasConceptScore W4308011662C55493867 @default.
- W4308011662 hasConceptScore W4308011662C61048295 @default.
- W4308011662 hasConceptScore W4308011662C62520636 @default.
- W4308011662 hasConceptScore W4308011662C86803240 @default.
- W4308011662 hasFunder F4320334789 @default.
- W4308011662 hasLocation W43080116621 @default.
- W4308011662 hasOpenAccess W4308011662 @default.
- W4308011662 hasPrimaryLocation W43080116621 @default.
- W4308011662 hasRelatedWork W1974037225 @default.
- W4308011662 hasRelatedWork W1989651157 @default.
- W4308011662 hasRelatedWork W2011582248 @default.
- W4308011662 hasRelatedWork W2049421402 @default.
- W4308011662 hasRelatedWork W2053193268 @default.
- W4308011662 hasRelatedWork W2070905182 @default.
- W4308011662 hasRelatedWork W2115718246 @default.
- W4308011662 hasRelatedWork W2232261751 @default.
- W4308011662 hasRelatedWork W3046193587 @default.
- W4308011662 hasRelatedWork W3119322097 @default.
- W4308011662 isParatext "false" @default.
- W4308011662 isRetracted "false" @default.
- W4308011662 workType "article" @default.