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- W4308016815 abstract "Abstract Extracellular vesicles (EVs) are increasingly recognized as an important mechanism for cell–cell interactions. Their role in fungi is still poorly understood and they have been isolated from only a handful of species. Here, we isolated and characterized EVs from Aureobasidium pullulans , a biotechnologically important black yeast-like fungus that is increasingly used for biocontrol of phytopathogenic fungi and bacteria. After optimization of the isolation protocol, characterization of EVs from A. pullulans by transmission electron microscopy (TEM) revealed a typical cup-shaped morphology and different subpopulations of EVs. These results were confirmed by nanoparticle tracking analysis (NTA), which revealed that A. pullulans produced 6.1 × 10 8 nanoparticles per milliliter of culture medium. Proteomic analysis of EVs detected 642 proteins. A small fraction of them had signal peptides for secretion and transmembrane domains. Proteins characteristic of different synthesis pathways were found, suggesting that EVs are synthesized by multiple pathways in A. pullulans . Enrichment analysis using Gene Ontology showed that most of the proteins found in the EVs were associated with primary metabolism. When sequencing the small RNA fraction of A. pullulans EVs, we found two hypothetical novel mil-RNAs. Finally, we tested the biocontrol potential of EVs from A. pullulans . The EVs did not inhibit the germination of spores of three important phytopathogenic fungi— Botrytis cinerea , Colletotrichum acutatum , and Penicillium expansum . However, exposure of grown cultures of C. acutatum and P. expansum to A. pullulans EVs resulted in visible changes in morphology of colonies. These preliminary results suggest that EVs may be part of the antagonistic activity of A. pullulans , which is so far only partially understood. Thus, the first isolation and characterization of EVs from A. pullulans provides a starting point for further studies of EVs in the biotechnologically important traits of the biocontrol black fungus A. pullulans in particular and in the biological role of fungal EVs in general." @default.
- W4308016815 created "2022-11-07" @default.
- W4308016815 creator A5031350262 @default.
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- W4308016815 creator A5073657395 @default.
- W4308016815 date "2022-11-01" @default.
- W4308016815 modified "2023-10-14" @default.
- W4308016815 title "Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans" @default.
- W4308016815 cites W1544767817 @default.
- W4308016815 cites W15885085 @default.
- W4308016815 cites W1839486704 @default.
- W4308016815 cites W1872329267 @default.
- W4308016815 cites W1875751185 @default.
- W4308016815 cites W1964693417 @default.
- W4308016815 cites W1967276739 @default.
- W4308016815 cites W1983563728 @default.
- W4308016815 cites W1986198073 @default.
- W4308016815 cites W1988780738 @default.
- W4308016815 cites W2001555960 @default.
- W4308016815 cites W2002220399 @default.
- W4308016815 cites W2009062449 @default.
- W4308016815 cites W2017426710 @default.
- W4308016815 cites W2018008972 @default.
- W4308016815 cites W2022159464 @default.
- W4308016815 cites W2027967242 @default.
- W4308016815 cites W2036983279 @default.
- W4308016815 cites W2043828929 @default.
- W4308016815 cites W2049513638 @default.
- W4308016815 cites W2052739924 @default.
- W4308016815 cites W2056952120 @default.
- W4308016815 cites W2067429517 @default.
- W4308016815 cites W2067850641 @default.
- W4308016815 cites W2080548543 @default.
- W4308016815 cites W2086076507 @default.
- W4308016815 cites W2093906189 @default.
- W4308016815 cites W2095124127 @default.
- W4308016815 cites W2105706954 @default.
- W4308016815 cites W2111840002 @default.
- W4308016815 cites W2112358553 @default.
- W4308016815 cites W2114083522 @default.
- W4308016815 cites W2125636530 @default.
- W4308016815 cites W2130305733 @default.
- W4308016815 cites W2145259682 @default.
- W4308016815 cites W2145821725 @default.
- W4308016815 cites W2152499975 @default.
- W4308016815 cites W2152733321 @default.
- W4308016815 cites W2152770371 @default.
- W4308016815 cites W2167246272 @default.
- W4308016815 cites W2170132701 @default.
- W4308016815 cites W2170852633 @default.
- W4308016815 cites W2410514074 @default.
- W4308016815 cites W2557357928 @default.
- W4308016815 cites W2570474521 @default.
- W4308016815 cites W2576450997 @default.
- W4308016815 cites W2587956115 @default.
- W4308016815 cites W2594025068 @default.
- W4308016815 cites W2737872074 @default.
- W4308016815 cites W2766153226 @default.
- W4308016815 cites W2768576987 @default.
- W4308016815 cites W2770375221 @default.
- W4308016815 cites W2787963369 @default.
- W4308016815 cites W2803645366 @default.
- W4308016815 cites W2900756811 @default.
- W4308016815 cites W2907183438 @default.
- W4308016815 cites W2910601935 @default.
- W4308016815 cites W2912470717 @default.
- W4308016815 cites W2921128942 @default.
- W4308016815 cites W2950094403 @default.
- W4308016815 cites W2967223910 @default.
- W4308016815 cites W2991626614 @default.
- W4308016815 cites W3002684303 @default.
- W4308016815 cites W3016535436 @default.
- W4308016815 cites W3017376547 @default.
- W4308016815 cites W3043111928 @default.
- W4308016815 cites W3043194344 @default.
- W4308016815 cites W3087089252 @default.
- W4308016815 cites W3093083291 @default.
- W4308016815 cites W3100348855 @default.
- W4308016815 cites W3127100983 @default.
- W4308016815 cites W3127251204 @default.
- W4308016815 cites W3159156019 @default.
- W4308016815 cites W3170143948 @default.
- W4308016815 cites W3174663187 @default.
- W4308016815 cites W3177714952 @default.
- W4308016815 cites W3196911446 @default.
- W4308016815 cites W3214675403 @default.
- W4308016815 cites W4206950245 @default.
- W4308016815 cites W4238118768 @default.
- W4308016815 cites W4254687493 @default.
- W4308016815 cites W4285752832 @default.
- W4308016815 cites W4211172120 @default.
- W4308016815 doi "https://doi.org/10.1186/s40694-022-00146-7" @default.
- W4308016815 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36320088" @default.
- W4308016815 hasPublicationYear "2022" @default.
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