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• W4308045176 abstract "Structural changes and neuropathology in the hypothalamus have been suggested to contribute to the non-motor manifestations of Huntington's disease (HD), a neurodegenerative disorder caused by an expanded cytosine-adenine-guanine (CAG) repeat in the huntingtin (HTT) gene. In this study, we investigated whether hypothalamic HTT expression causes transcriptional changes. Hypothalamic RNA was isolated from two different HD mouse models and their littermate controls; BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. The mHTT and wtHTT groups showed the highest number of differentially expressed genes compared to the BACHD mouse model. Gene Set Enrichment Analysis (GSEA) with leading-edge analysis showed that suppressed sterol- and cholesterol metabolism were shared between hypothalamic wtHTT and mHTT overexpression. Most distinctive for mHTT overexpression was the suppression of neuroendocrine networks, in which qRT-PCR validation confirmed significant downregulation of neuropeptides with roles in feeding behavior; hypocretin neuropeptide precursor (Hcrt), tachykinin receptor 3 (Tacr3), cocaine and amphetamine-regulated transcript (Cart) and catecholamine-related biological processes; dopa decarboxylase (Ddc), histidine decarboxylase (Hdc), tyrosine hydroxylase (Th), and vasoactive intestinal peptide (Vip). In BACHD mice, few hypothalamic genes were differentially expressed compared to age-matched WT controls. However, GSEA indicated an enrichment of inflammatory- and gonadotropin-related processes at 10 months. In conclusion, we show that both wtHTT and mHTT overexpression change hypothalamic transcriptome profile, specifically mHTT, altering neuroendocrine circuits. In contrast, the ubiquitous expression of full-length mHTT in the BACHD hypothalamus moderately affects the transcriptomic profile." @default.
• W4308045176 created "2022-11-07" @default.
• W4308045176 creator A5003258012 @default.
• W4308045176 creator A5003327629 @default.
• W4308045176 creator A5046104862 @default.
• W4308045176 creator A5046191707 @default.
• W4308045176 creator A5060025749 @default.
• W4308045176 creator A5083971697 @default.
• W4308045176 creator A5089901938 @default.
• W4308045176 date "2022-11-03" @default.
• W4308045176 modified "2023-10-05" @default.
• W4308045176 title "Microarray profiling of hypothalamic gene expression changes in Huntington’s disease mouse models" @default.
• W4308045176 cites W1040888847 @default.
• W4308045176 cites W1164968108 @default.
• W4308045176 cites W1493631176 @default.
• W4308045176 cites W1511961767 @default.
• W4308045176 cites W1703545573 @default.
• W4308045176 cites W1713405122 @default.
• W4308045176 cites W1757606876 @default.
• W4308045176 cites W1895552261 @default.
• W4308045176 cites W1905429970 @default.
• W4308045176 cites W1965362486 @default.
• W4308045176 cites W1967627236 @default.
• W4308045176 cites W1969461857 @default.
• W4308045176 cites W1970770490 @default.
• W4308045176 cites W1972673843 @default.
• W4308045176 cites W1973765221 @default.
• W4308045176 cites W1978623496 @default.
• W4308045176 cites W1987519006 @default.
• W4308045176 cites W1990008286 @default.
• W4308045176 cites W1990783840 @default.
• W4308045176 cites W1993418561 @default.
• W4308045176 cites W2000693195 @default.
• W4308045176 cites W2003955696 @default.
• W4308045176 cites W2007157248 @default.
• W4308045176 cites W2007158777 @default.
• W4308045176 cites W2009115851 @default.
• W4308045176 cites W2015041659 @default.
• W4308045176 cites W2016742032 @default.
• W4308045176 cites W2020541351 @default.
• W4308045176 cites W2025131475 @default.
• W4308045176 cites W2028633253 @default.
• W4308045176 cites W2031722229 @default.
• W4308045176 cites W2032794571 @default.
• W4308045176 cites W2032864658 @default.
• W4308045176 cites W2035618305 @default.
• W4308045176 cites W2049861653 @default.
• W4308045176 cites W2058321088 @default.
• W4308045176 cites W2058759341 @default.
• W4308045176 cites W2069665729 @default.
• W4308045176 cites W2074980206 @default.
• W4308045176 cites W2079124378 @default.
• W4308045176 cites W2084636668 @default.
• W4308045176 cites W2094074268 @default.
• W4308045176 cites W2100621669 @default.
• W4308045176 cites W2104117687 @default.
• W4308045176 cites W2107277218 @default.
• W4308045176 cites W2107514471 @default.
• W4308045176 cites W2110017524 @default.
• W4308045176 cites W2110256992 @default.
• W4308045176 cites W2113964087 @default.
• W4308045176 cites W2116634113 @default.
• W4308045176 cites W2121642311 @default.
• W4308045176 cites W2122131368 @default.
• W4308045176 cites W2127294578 @default.
• W4308045176 cites W2130410032 @default.
• W4308045176 cites W2133014036 @default.
• W4308045176 cites W2134401364 @default.
• W4308045176 cites W2137023268 @default.
• W4308045176 cites W2137983766 @default.
• W4308045176 cites W2138304913 @default.
• W4308045176 cites W2144934068 @default.
• W4308045176 cites W2146103593 @default.
• W4308045176 cites W2146512944 @default.
• W4308045176 cites W2158217645 @default.
• W4308045176 cites W2162098634 @default.
• W4308045176 cites W2180449164 @default.
• W4308045176 cites W2192080449 @default.
• W4308045176 cites W2211108236 @default.
• W4308045176 cites W2241985777 @default.
• W4308045176 cites W2291779372 @default.
• W4308045176 cites W2417862589 @default.
• W4308045176 cites W2567620261 @default.
• W4308045176 cites W2591844322 @default.
• W4308045176 cites W2618178578 @default.
• W4308045176 cites W2743474796 @default.
• W4308045176 cites W2755227731 @default.
• W4308045176 cites W2883225111 @default.
• W4308045176 cites W2969181876 @default.
• W4308045176 cites W2976265374 @default.
• W4308045176 cites W3004881993 @default.
• W4308045176 cites W3099018540 @default.
• W4308045176 cites W3160595780 @default.
• W4308045176 cites W3171911409 @default.
• W4308045176 cites W3175752499 @default.
• W4308045176 cites W3181413218 @default.
• W4308045176 cites W3199523891 @default.
• W4308045176 cites W4206762599 @default.
• W4308045176 cites W4210584483 @default.
• W4308045176 cites W4211110682 @default.

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