Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308120833> ?p ?o ?g. }
- W4308120833 endingPage "5210" @default.
- W4308120833 startingPage "5210" @default.
- W4308120833 abstract "Intracellular mono-ADP-ribosyltransferases (mono-ARTs) catalyze the covalent attachment of a single ADP-ribose molecule to protein substrates, thus regulating their functions. PARP10 is a soluble mono-ART involved in the modulation of intracellular signaling, metabolism and apoptosis. PARP10 also participates in the regulation of the G1- and S-phase of the cell cycle. However, the role of this enzyme in G2/M progression is not defined. In this study, we found that genetic ablation, protein depletion and pharmacological inhibition of PARP10 cause a delay in the G2/M transition of the cell cycle. Moreover, we found that the mitotic kinase Aurora-A, a previously identified PARP10 substrate, is actively mono-ADP-ribosylated (MARylated) during G2/M transition in a PARP10-dependent manner. Notably, we showed that PARP10-mediated MARylation of Aurora-A enhances the activity of the kinase in vitro. Consistent with an impairment in the endogenous activity of Aurora-A, cells lacking PARP10 show a decreased localization of the kinase on the centrosomes and mitotic spindle during G2/M progression. Taken together, our data provide the first evidence of a direct role played by PARP10 in the progression of G2 and mitosis, an event that is strictly correlated to the endogenous MARylation of Aurora-A, thus proposing a novel mechanism for the modulation of Aurora-A kinase activity." @default.
- W4308120833 created "2022-11-08" @default.
- W4308120833 creator A5001789850 @default.
- W4308120833 creator A5013106468 @default.
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- W4308120833 creator A5063991133 @default.
- W4308120833 creator A5066063513 @default.
- W4308120833 creator A5076045755 @default.
- W4308120833 date "2022-10-24" @default.
- W4308120833 modified "2023-09-25" @default.
- W4308120833 title "PARP10 Mediates Mono-ADP-Ribosylation of Aurora-A Regulating G2/M Transition of the Cell Cycle" @default.
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