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• W4308149691 endingPage "122056" @default.
• W4308149691 startingPage "122056" @default.
• W4308149691 abstract "We have found that tris (2,2'-bipyridyl) ruthenium (II) (Ru(bpy)32+) possesses a high photo-induced oxidase-like activity and is capable of catalyzing the color reaction of 3,3',5,5'-tetramethylbenzidine (TMB) with dissolved oxygen. Ru(bpy)32+ has a catalytic constant (Kcat) that is twice as high as that of fluorescein, 170 and 275-fold higher than that of 9-mesityl-10-methyl acridine and Eosin Y, respectively. Electron spin resonance spectroscopy (ESR) and radical scavenging experiments have verified the major active radicals involved in the color reaction are •OH. A colorimetric biothiol assay has been successfully developed for the oxidase-like activity of Ru(bpy)32+ can be suppressed by sulfhydryl compounds. A linear dependence between the decrease in absorbance and the logarithm of thiol concentrations can be found ranging from 5.0 to 50 μM, with a detection limit of 1.0 μM. This work reveals a new oxidase mimic with high catalytic activity and will facilitate the utilization of this oxidase mimic in biochemical analysis." @default.
• W4308149691 created "2022-11-08" @default.
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• W4308149691 date "2023-02-01" @default.
• W4308149691 modified "2023-09-26" @default.
• W4308149691 title "Ru(bpy)32+ as a photoinduced oxidase mimic for colorimetric detection of biothiols" @default.
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• W4308149691 doi "https://doi.org/10.1016/j.saa.2022.122056" @default.
• W4308149691 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36327804" @default.
• W4308149691 hasPublicationYear "2023" @default.
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