Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308180765> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W4308180765 endingPage "2110" @default.
- W4308180765 startingPage "2102" @default.
- W4308180765 abstract "Recent studies have shown that mitochondrial morphology can modulate organelle function and greatly affect stem cell behavior, thus affecting tissue homeostasis. As such, we previously showed that the accumulation of fragmented mitochondria in aged Drosophila ovarian germline stem cells (GSCs) contributes to age-dependent GSC loss. However, standard immunofluorescence methods to examine mitochondrial morphology yield images with insufficient resolution for rigorous analysis, while 3-dimensional electron microscopy examination of mitochondrial morphology is labor intensive and allows only limited sampling of mitochondria. To overcome these issues, we utilized the expansion microscopy technique to expand GSC samples by 4-fold in combination with mitochondrial immunofluorescence labeling. Here, we present a simple, inexpensive method for nanoscale optical imaging of mitochondria in the germline. This protocol may be beneficial for studies that require visualization of mitochondria or other fine subcellular structures in the Drosophila ovary." @default.
- W4308180765 created "2022-11-08" @default.
- W4308180765 creator A5027637533 @default.
- W4308180765 creator A5028987869 @default.
- W4308180765 creator A5040730537 @default.
- W4308180765 creator A5074101286 @default.
- W4308180765 date "2022-11-17" @default.
- W4308180765 modified "2023-10-14" @default.
- W4308180765 title "Expansion Microscopy‐based imaging for visualization of mitochondria in <i>Drosophila</i> ovarian germline stem cells" @default.
- W4308180765 cites W1600103888 @default.
- W4308180765 cites W2008266179 @default.
- W4308180765 cites W2031353653 @default.
- W4308180765 cites W2090581673 @default.
- W4308180765 cites W2096373575 @default.
- W4308180765 cites W2346126921 @default.
- W4308180765 cites W2402960573 @default.
- W4308180765 cites W2406725268 @default.
- W4308180765 cites W2460281481 @default.
- W4308180765 cites W2741475039 @default.
- W4308180765 cites W2885194328 @default.
- W4308180765 cites W2898666867 @default.
- W4308180765 cites W2901571157 @default.
- W4308180765 cites W3043370561 @default.
- W4308180765 cites W3180817903 @default.
- W4308180765 doi "https://doi.org/10.1002/2211-5463.13506" @default.
- W4308180765 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36331359" @default.
- W4308180765 hasPublicationYear "2022" @default.
- W4308180765 type Work @default.
- W4308180765 citedByCount "1" @default.
- W4308180765 countsByYear W43081807652023 @default.
- W4308180765 crossrefType "journal-article" @default.
- W4308180765 hasAuthorship W4308180765A5027637533 @default.
- W4308180765 hasAuthorship W4308180765A5028987869 @default.
- W4308180765 hasAuthorship W4308180765A5040730537 @default.
- W4308180765 hasAuthorship W4308180765A5074101286 @default.
- W4308180765 hasBestOaLocation W43081807651 @default.
- W4308180765 hasConcept C104317684 @default.
- W4308180765 hasConcept C109825262 @default.
- W4308180765 hasConcept C1491633281 @default.
- W4308180765 hasConcept C15526883 @default.
- W4308180765 hasConcept C159654299 @default.
- W4308180765 hasConcept C168240541 @default.
- W4308180765 hasConcept C2778236600 @default.
- W4308180765 hasConcept C2778324911 @default.
- W4308180765 hasConcept C2779178360 @default.
- W4308180765 hasConcept C28328180 @default.
- W4308180765 hasConcept C28859421 @default.
- W4308180765 hasConcept C54355233 @default.
- W4308180765 hasConcept C86803240 @default.
- W4308180765 hasConcept C95444343 @default.
- W4308180765 hasConceptScore W4308180765C104317684 @default.
- W4308180765 hasConceptScore W4308180765C109825262 @default.
- W4308180765 hasConceptScore W4308180765C1491633281 @default.
- W4308180765 hasConceptScore W4308180765C15526883 @default.
- W4308180765 hasConceptScore W4308180765C159654299 @default.
- W4308180765 hasConceptScore W4308180765C168240541 @default.
- W4308180765 hasConceptScore W4308180765C2778236600 @default.
- W4308180765 hasConceptScore W4308180765C2778324911 @default.
- W4308180765 hasConceptScore W4308180765C2779178360 @default.
- W4308180765 hasConceptScore W4308180765C28328180 @default.
- W4308180765 hasConceptScore W4308180765C28859421 @default.
- W4308180765 hasConceptScore W4308180765C54355233 @default.
- W4308180765 hasConceptScore W4308180765C86803240 @default.
- W4308180765 hasConceptScore W4308180765C95444343 @default.
- W4308180765 hasIssue "12" @default.
- W4308180765 hasLocation W43081807651 @default.
- W4308180765 hasLocation W43081807652 @default.
- W4308180765 hasLocation W43081807653 @default.
- W4308180765 hasLocation W43081807654 @default.
- W4308180765 hasOpenAccess W4308180765 @default.
- W4308180765 hasPrimaryLocation W43081807651 @default.
- W4308180765 hasRelatedWork W1481440753 @default.
- W4308180765 hasRelatedWork W1979374213 @default.
- W4308180765 hasRelatedWork W1990022233 @default.
- W4308180765 hasRelatedWork W2006540291 @default.
- W4308180765 hasRelatedWork W2042187600 @default.
- W4308180765 hasRelatedWork W2090450622 @default.
- W4308180765 hasRelatedWork W2093785836 @default.
- W4308180765 hasRelatedWork W2418253448 @default.
- W4308180765 hasRelatedWork W4386741245 @default.
- W4308180765 hasRelatedWork W4387354525 @default.
- W4308180765 hasVolume "12" @default.
- W4308180765 isParatext "false" @default.
- W4308180765 isRetracted "false" @default.
- W4308180765 workType "article" @default.