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- W4308188315 abstract "Abstract This 25‐parameter, 22‐color full spectrum flow cytometry panel was designed and optimized for the comprehensive enumeration and functional characterization of innate lymphoid cell (ILC) subsets in mouse tissues. The panel presented here allows the discrimination of ILC progenitors (ILCP), ILC1, ILC2, NCR + ILC3, NCR − ILC3, CCR6 + lymphoid tissue‐inducer (LTi)‐like ILC3 and mature natural killer (NK) cell populations. Further characterization of ILC and NK cell functional profiles in response to stimulation is provided by the inclusion of subset‐specific cytokine markers, and proliferation markers. Development and optimization of this panel was performed on freshly isolated cells from adult BALB/c lungs and small intestine lamina propria, and ex vivo stimulation with phorbol 12‐myrisate 13‐acetate, ionomycin, and pro‐ILC activating cytokines." @default.
- W4308188315 created "2022-11-09" @default.
- W4308188315 creator A5024908152 @default.
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- W4308188315 date "2022-11-04" @default.
- W4308188315 modified "2023-09-30" @default.
- W4308188315 title "OMIP‐086: Full spectrum flow cytometry for <scp>high‐dimensional</scp> immunophenotyping of mouse innate lymphoid cells" @default.
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- W4308188315 doi "https://doi.org/10.1002/cyto.a.24702" @default.
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