Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308198325> ?p ?o ?g. }
- W4308198325 endingPage "1630" @default.
- W4308198325 startingPage "1630" @default.
- W4308198325 abstract "Mitochondrial (mt) DNA and nuclear (n) DNA have known structures and roles in cells; however, they are rarely compared under specific conditions such as oxidative or degenerative environments that can create damage to the DNA base moieties. Six purine lesions were ascertained in the mtDNA of wild type (wt) CSA (CS3BE-wtCSA) and wtCSB (CS1AN-wtCSB) cells and defective counterparts CS3BE and CS1AN in comparison with the corresponding total (t) DNA (t = n + mt). In particular, the four 5',8-cyclopurine (cPu) and the two 8-oxo-purine (8-oxo-Pu) lesions were accurately quantified by LC-MS/MS analysis using isotopomeric internal standards after an enzymatic digestion procedure. The 8-oxo-Pu levels were found to be in the range of 25-50 lesions/107 nucleotides in both the mtDNA and tDNA. The four cPu were undetectable in the mtDNA both in defective cells and in the wt counterparts (CSA and CSB), contrary to their detection in tDNA, indicating a nonappearance of hydroxyl radical (HO•) reactivity within the mtDNA. In order to assess the HO• reactivity towards purine nucleobases in the two genetic materials, we performed γ-radiolysis experiments coupled with the 8-oxo-Pu and cPu quantifications on isolated mtDNA and tDNA from wtCSB cells. In the latter experiments, all six purine lesions were detected in both of the DNA, showing a higher resistance to HO• attack in the case of mtDNA compared with tDNA, likely due to their different DNA helical topology influencing the relative abundance of the lesions." @default.
- W4308198325 created "2022-11-09" @default.
- W4308198325 creator A5001357889 @default.
- W4308198325 creator A5023929901 @default.
- W4308198325 creator A5045380690 @default.
- W4308198325 creator A5051591358 @default.
- W4308198325 creator A5059013256 @default.
- W4308198325 creator A5067024672 @default.
- W4308198325 creator A5076355182 @default.
- W4308198325 date "2022-11-03" @default.
- W4308198325 modified "2023-10-03" @default.
- W4308198325 title "Assessing the Formation of Purine Lesions in Mitochondrial DNA of Cockayne Syndrome Cells" @default.
- W4308198325 cites W1501611632 @default.
- W4308198325 cites W1512013436 @default.
- W4308198325 cites W1532652169 @default.
- W4308198325 cites W1563822242 @default.
- W4308198325 cites W1815297412 @default.
- W4308198325 cites W1926950498 @default.
- W4308198325 cites W1983598709 @default.
- W4308198325 cites W1984060145 @default.
- W4308198325 cites W1984532604 @default.
- W4308198325 cites W1987954870 @default.
- W4308198325 cites W1987960916 @default.
- W4308198325 cites W1987998070 @default.
- W4308198325 cites W1991682155 @default.
- W4308198325 cites W1991952977 @default.
- W4308198325 cites W1993149192 @default.
- W4308198325 cites W1999620455 @default.
- W4308198325 cites W2007969271 @default.
- W4308198325 cites W2008592200 @default.
- W4308198325 cites W2029434476 @default.
- W4308198325 cites W2030666445 @default.
- W4308198325 cites W2040989554 @default.
- W4308198325 cites W2046082575 @default.
- W4308198325 cites W2058659691 @default.
- W4308198325 cites W2059891246 @default.
- W4308198325 cites W2068337326 @default.
- W4308198325 cites W2081745487 @default.
- W4308198325 cites W2087736884 @default.
- W4308198325 cites W2089008349 @default.
- W4308198325 cites W2101802274 @default.
- W4308198325 cites W2109291482 @default.
- W4308198325 cites W2113912029 @default.
- W4308198325 cites W2114443210 @default.
- W4308198325 cites W2122341467 @default.
- W4308198325 cites W2131415852 @default.
- W4308198325 cites W2133770419 @default.
- W4308198325 cites W2147993424 @default.
- W4308198325 cites W2159383423 @default.
- W4308198325 cites W2169186429 @default.
- W4308198325 cites W2170597525 @default.
- W4308198325 cites W2235117179 @default.
- W4308198325 cites W2284273064 @default.
- W4308198325 cites W2414091614 @default.
- W4308198325 cites W2531550612 @default.
- W4308198325 cites W2607644287 @default.
- W4308198325 cites W2789312639 @default.
- W4308198325 cites W2806941167 @default.
- W4308198325 cites W2900892676 @default.
- W4308198325 cites W2906404989 @default.
- W4308198325 cites W2931837038 @default.
- W4308198325 cites W2947923834 @default.
- W4308198325 cites W2948369867 @default.
- W4308198325 cites W2957998011 @default.
- W4308198325 cites W2981478258 @default.
- W4308198325 cites W3003715287 @default.
- W4308198325 cites W3009224479 @default.
- W4308198325 cites W3013352992 @default.
- W4308198325 cites W3037012236 @default.
- W4308198325 cites W3041310624 @default.
- W4308198325 cites W3126009536 @default.
- W4308198325 cites W3164138302 @default.
- W4308198325 cites W3166071930 @default.
- W4308198325 cites W3173054704 @default.
- W4308198325 cites W3173605490 @default.
- W4308198325 cites W3176723802 @default.
- W4308198325 cites W3179257488 @default.
- W4308198325 cites W4223518507 @default.
- W4308198325 cites W4223555747 @default.
- W4308198325 cites W4248078302 @default.
- W4308198325 cites W4289986159 @default.
- W4308198325 doi "https://doi.org/10.3390/biom12111630" @default.
- W4308198325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36358980" @default.
- W4308198325 hasPublicationYear "2022" @default.
- W4308198325 type Work @default.
- W4308198325 citedByCount "0" @default.
- W4308198325 crossrefType "journal-article" @default.
- W4308198325 hasAuthorship W4308198325A5001357889 @default.
- W4308198325 hasAuthorship W4308198325A5023929901 @default.
- W4308198325 hasAuthorship W4308198325A5045380690 @default.
- W4308198325 hasAuthorship W4308198325A5051591358 @default.
- W4308198325 hasAuthorship W4308198325A5059013256 @default.
- W4308198325 hasAuthorship W4308198325A5067024672 @default.
- W4308198325 hasAuthorship W4308198325A5076355182 @default.
- W4308198325 hasBestOaLocation W43081983251 @default.
- W4308198325 hasConcept C104317684 @default.
- W4308198325 hasConcept C115960442 @default.
- W4308198325 hasConcept C143425029 @default.