FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308200582> ?p ?o ?g. }

• W4308200582 endingPage "406" @default.
• W4308200582 startingPage "397" @default.
• W4308200582 abstract "Abstract. Objective: The focus of this study was the six-transmembrane epithelial antigen of the prostate 4 (STEAP4) gene, on the basis of the cloned goat STEAP4 gene sequence. Its molecular and expression characteristics were analyzed, and its influence on the differentiation of goat subcutaneous adipocytes was explored through overexpression. Method: Reverse-transcription PCR (RT-PCR) was used to clone the goat STEAP4 sequence, and online tools were used to analyze the molecular characteristic. Real-time quantitative PCR (qPCR) was used to detect the expression level of STEAP4 in goat tissues and subcutaneous adipocyte differentiation. Liposome transfection, BODIPY, Oil Red O staining, and qPCR were used to explore the effect of overexpression of STEAP4 on adipocyte differentiation. Results: The cloned goat STEAP4 gene sequence was 1388 bp, and the complete coding sequence (CDS) region was 1197 bp, which encoded a total of 398 amino acids. Compared with the predicted sequence (XM_005679300.3), there were three base mutations in the CDS region of goat STEAP4, A188G, T281C, and A507G. Among them, A507G changed the amino acid at position 170 from Ile to Val. Analysis of the physical and chemical properties of the protein showed that STEAP4 was a stable hydrophilic basic protein. STEAP4 gene expression level was highest in goat liver tissue (P<0.01), followed by lung and back subcutaneous adipose tissue. STEAP4 showed different expression levels in goat subcutaneous adipocytes at different times during the induction of differentiation. The expression in the late stage of differentiation was higher than that before differentiation and lowest at 12 h (P<0.01). Overexpression of STEAP4 promoted the accumulation of intracellular lipid droplets; C/EBPβ (CCAAT enhancer binding protein) was extremely significantly up-regulated (P<0.01), and aP2 (fatty acid binding protein) was significantly up-regulated (P<0.05). Conclusion: Overexpression of STEAP4 could promote the differentiation of goat subcutaneous preadipocytes. This study lays the foundation for an in-depth study of the role of STEAP4 in goat lipid deposition." @default.
• W4308200582 created "2022-11-09" @default.
• W4308200582 creator A5003866201 @default.
• W4308200582 creator A5017938965 @default.
• W4308200582 creator A5022526821 @default.
• W4308200582 creator A5049341927 @default.
• W4308200582 creator A5062110178 @default.
• W4308200582 creator A5062371490 @default.
• W4308200582 creator A5066102428 @default.
• W4308200582 creator A5091610124 @default.
• W4308200582 date "2022-11-04" @default.
• W4308200582 modified "2023-10-17" @default.
• W4308200582 title "Overexpression of goat <i>STEAP4</i> promotes the differentiation of subcutaneous adipocytes" @default.
• W4308200582 cites W1974544823 @default.
• W4308200582 cites W1975543439 @default.
• W4308200582 cites W1985689804 @default.
• W4308200582 cites W1987120082 @default.
• W4308200582 cites W1996808945 @default.
• W4308200582 cites W2001904774 @default.
• W4308200582 cites W2005381157 @default.
• W4308200582 cites W2027090731 @default.
• W4308200582 cites W2045320454 @default.
• W4308200582 cites W2060978273 @default.
• W4308200582 cites W2064702079 @default.
• W4308200582 cites W2069774708 @default.
• W4308200582 cites W2084428683 @default.
• W4308200582 cites W2097791054 @default.
• W4308200582 cites W2097899015 @default.
• W4308200582 cites W2098141705 @default.
• W4308200582 cites W2133067519 @default.
• W4308200582 cites W2134888531 @default.
• W4308200582 cites W2135183404 @default.
• W4308200582 cites W2138362261 @default.
• W4308200582 cites W2140065782 @default.
• W4308200582 cites W2154757713 @default.
• W4308200582 cites W2158314006 @default.
• W4308200582 cites W2159090847 @default.
• W4308200582 cites W2166593442 @default.
• W4308200582 cites W2331136745 @default.
• W4308200582 cites W2542964897 @default.
• W4308200582 cites W2765107560 @default.
• W4308200582 cites W2892416438 @default.
• W4308200582 cites W2896690111 @default.
• W4308200582 cites W2981435678 @default.
• W4308200582 cites W3128121638 @default.
• W4308200582 cites W4232264301 @default.
• W4308200582 doi "https://doi.org/10.5194/aab-65-397-2022" @default.
• W4308200582 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36415757" @default.
• W4308200582 hasPublicationYear "2022" @default.
• W4308200582 type Work @default.
• W4308200582 citedByCount "1" @default.
• W4308200582 countsByYear W43082005822023 @default.
• W4308200582 crossrefType "journal-article" @default.
• W4308200582 hasAuthorship W4308200582A5003866201 @default.
• W4308200582 hasAuthorship W4308200582A5017938965 @default.
• W4308200582 hasAuthorship W4308200582A5022526821 @default.
• W4308200582 hasAuthorship W4308200582A5049341927 @default.
• W4308200582 hasAuthorship W4308200582A5062110178 @default.
• W4308200582 hasAuthorship W4308200582A5062371490 @default.
• W4308200582 hasAuthorship W4308200582A5066102428 @default.
• W4308200582 hasAuthorship W4308200582A5091610124 @default.
• W4308200582 hasBestOaLocation W43082005821 @default.
• W4308200582 hasConcept C104317684 @default.
• W4308200582 hasConcept C122927707 @default.
• W4308200582 hasConcept C148738053 @default.
• W4308200582 hasConcept C153911025 @default.
• W4308200582 hasConcept C171089720 @default.
• W4308200582 hasConcept C185592680 @default.
• W4308200582 hasConcept C2776175234 @default.
• W4308200582 hasConcept C2780806160 @default.
• W4308200582 hasConcept C54009773 @default.
• W4308200582 hasConcept C55493867 @default.
• W4308200582 hasConcept C86803240 @default.
• W4308200582 hasConcept C91779695 @default.
• W4308200582 hasConceptScore W4308200582C104317684 @default.
• W4308200582 hasConceptScore W4308200582C122927707 @default.
• W4308200582 hasConceptScore W4308200582C148738053 @default.
• W4308200582 hasConceptScore W4308200582C153911025 @default.
• W4308200582 hasConceptScore W4308200582C171089720 @default.
• W4308200582 hasConceptScore W4308200582C185592680 @default.
• W4308200582 hasConceptScore W4308200582C2776175234 @default.
• W4308200582 hasConceptScore W4308200582C2780806160 @default.
• W4308200582 hasConceptScore W4308200582C54009773 @default.
• W4308200582 hasConceptScore W4308200582C55493867 @default.
• W4308200582 hasConceptScore W4308200582C86803240 @default.
• W4308200582 hasConceptScore W4308200582C91779695 @default.
• W4308200582 hasFunder F4320321001 @default.
• W4308200582 hasFunder F4320333337 @default.
• W4308200582 hasFunder F4320335787 @default.
• W4308200582 hasIssue "4" @default.
• W4308200582 hasLocation W43082005821 @default.
• W4308200582 hasLocation W43082005822 @default.
• W4308200582 hasLocation W43082005823 @default.
• W4308200582 hasOpenAccess W4308200582 @default.
• W4308200582 hasPrimaryLocation W43082005821 @default.
• W4308200582 hasRelatedWork W2025315694 @default.
• W4308200582 hasRelatedWork W2065646454 @default.
• W4308200582 hasRelatedWork W2069246662 @default.

• next