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• W4308222787 abstract "Choroid plexus, located in brain ventricles, is the site of blood-cerebrospinal fluid barrier that contains endothelial cells and an epithelial monolayer separated by stroma. We established a two-cell-type model of the human choroid plexus consisting of immortalized endothelial cells (iHCPEnC) and epithelial papilloma (HIBCPP) cells grown on opposite sides of filter supports. In this protocol, we describe the preparation of this model, the measurement of transepithelial electrical resistance (TEER), and immunofluorescence imaging-based analysis to determine the barrier function. For complete details on the use and execution of this protocol, please refer to Muranyi et al. (2022)." @default.
• W4308222787 created "2022-11-09" @default.
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• W4308222787 date "2022-12-01" @default.
• W4308222787 modified "2023-10-14" @default.
• W4308222787 title "Protocol for the setup and use of a human choroid plexus endothelial-epithelial two-cell-type in vitro model" @default.
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• W4308222787 doi "https://doi.org/10.1016/j.xpro.2022.101816" @default.
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