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- W4308232444 abstract "Abstract Background: root canal obturation is performed by gutta-percha cones and sealer. Therefore, these materials specially sealers, must be biocompatible. This study aimed to investigate the cytotoxicity and mineralization activity of two calcium silicate-based sealers (Endoseal MTA and Ceraseal) and an epoxy resin-based sealer (AH26). Methods: in this experiment, the toxicity of Endoseal MTA, Ceraseal, and AH26 on human gingival fibroblast cells was examined using Methyl-Thiazol-Tetrazolium assay at time intervals of 24, 48, 72, and 120 hours. The mineralization activity of sealers was evaluated by Alizarin Red Staining assay. Data analysis was carried out using one-way ANOVA and Tukey’s post-test at a significance level of less than 0.05. Results: cytotoxicity of sealers decreased gradually (P<0.0001). AH26 showed the highest level of cytotoxicity (P<0.001). in terms of cytotoxicity, no considerable differences were observed between the two calcium silicate-based sealers. AH26 showed the lowest mineralization activity (P<0.0001). among the calcium silicate-based sealers, mineralization and formation of calcium nodules were more frequently observed in the Endoseal MTA group (P<0.001). Conclusion: the examined calcium silicate-based sealers had less cytotoxicity and higher mineralization activity than the resin-based sealer (AH26). There was negligible difference between the cytotoxicity of the two calcium silicate-based, but the cell mineralization caused by endoseal MTA was higher." @default.
- W4308232444 created "2022-11-09" @default.
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- W4308232444 date "2022-11-03" @default.
- W4308232444 modified "2023-10-17" @default.
- W4308232444 title "Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast cells" @default.
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- W4308232444 doi "https://doi.org/10.21203/rs.3.rs-2202823/v1" @default.
- W4308232444 hasPublicationYear "2022" @default.
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