FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308295089> ?p ?o ?g. }

• W4308295089 endingPage "111523" @default.
• W4308295089 startingPage "111523" @default.
• W4308295089 abstract "CRISPR/Cas9 genome-editing technology has revolutionized plant science and holds enormous promise for crop improvement. The exploration of this system received much attention regarding plant genome editing. Here, by editing the NtPDS gene in tobacco, we first verified that incorporating an OsU3-tRNA promoter combination into the CRISPR/Cas9 system contributed to the highest editing efficiency, as the sgRNA expression level was greater than that resulting from the AtU6-tRNA and AtU6 promoters. Then, we optimized the existing tobacco CRISPR/Cas9 system, pORE-Cas9, by using the OsU3-tRNA promoter combination instead of AtU6 and by fusing an AtUb10-Ros1 expression cassette to the T-DNA to monitor the transgene events. The new system was named pOREU3TR. As expected, 49 transgene-free and homozygous gene-edited green plants were effectively screened in the T1 generation as a result of editing the NtLHT1 gene in tobacco, and the plant height and the contents of most free amino acids in the leaves of the T2 mutant plants were significantly different from those in the leaves of WT plants, demonstrating the high efficiency of the new editing system. This OsU3-tRNA-sgRNA/AtUb10-Ros1 system provides essential improvements for increasing the efficiency of plant genome editing." @default.
• W4308295089 created "2022-11-10" @default.
• W4308295089 creator A5002202297 @default.
• W4308295089 creator A5006641884 @default.
• W4308295089 creator A5014546889 @default.
• W4308295089 creator A5018244700 @default.
• W4308295089 creator A5019114824 @default.
• W4308295089 creator A5051386160 @default.
• W4308295089 creator A5051746234 @default.
• W4308295089 creator A5062199282 @default.
• W4308295089 creator A5064478014 @default.
• W4308295089 creator A5068899977 @default.
• W4308295089 creator A5070622545 @default.
• W4308295089 creator A5071842934 @default.
• W4308295089 creator A5089109826 @default.
• W4308295089 date "2023-01-01" @default.
• W4308295089 modified "2023-10-18" @default.
• W4308295089 title "Highly efficient transgene-free genome editing in tobacco using an optimized CRISPR/Cas9 system, pOREU3TR" @default.
• W4308295089 cites W1510606824 @default.
• W4308295089 cites W1885181197 @default.
• W4308295089 cites W1922401563 @default.
• W4308295089 cites W1937054661 @default.
• W4308295089 cites W1965853301 @default.
• W4308295089 cites W1968342623 @default.
• W4308295089 cites W1982802227 @default.
• W4308295089 cites W1989421353 @default.
• W4308295089 cites W1992783297 @default.
• W4308295089 cites W2003240933 @default.
• W4308295089 cites W2008766717 @default.
• W4308295089 cites W2065661188 @default.
• W4308295089 cites W2076579920 @default.
• W4308295089 cites W2091861785 @default.
• W4308295089 cites W2135599428 @default.
• W4308295089 cites W2147114967 @default.
• W4308295089 cites W2217013086 @default.
• W4308295089 cites W2404129846 @default.
• W4308295089 cites W2604596552 @default.
• W4308295089 cites W2605561909 @default.
• W4308295089 cites W2885871006 @default.
• W4308295089 cites W2895213500 @default.
• W4308295089 cites W2896311550 @default.
• W4308295089 cites W2936275183 @default.
• W4308295089 cites W2950380007 @default.
• W4308295089 cites W2963358556 @default.
• W4308295089 cites W2980549983 @default.
• W4308295089 cites W3027701063 @default.
• W4308295089 cites W3108564058 @default.
• W4308295089 cites W3133208309 @default.
• W4308295089 cites W3133801250 @default.
• W4308295089 cites W3193550763 @default.
• W4308295089 cites W3202852589 @default.
• W4308295089 cites W3211019322 @default.
• W4308295089 doi "https://doi.org/10.1016/j.plantsci.2022.111523" @default.
• W4308295089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36334622" @default.
• W4308295089 hasPublicationYear "2023" @default.
• W4308295089 type Work @default.
• W4308295089 citedByCount "3" @default.
• W4308295089 countsByYear W43082950892023 @default.
• W4308295089 crossrefType "journal-article" @default.
• W4308295089 hasAuthorship W4308295089A5002202297 @default.
• W4308295089 hasAuthorship W4308295089A5006641884 @default.
• W4308295089 hasAuthorship W4308295089A5014546889 @default.
• W4308295089 hasAuthorship W4308295089A5018244700 @default.
• W4308295089 hasAuthorship W4308295089A5019114824 @default.
• W4308295089 hasAuthorship W4308295089A5051386160 @default.
• W4308295089 hasAuthorship W4308295089A5051746234 @default.
• W4308295089 hasAuthorship W4308295089A5062199282 @default.
• W4308295089 hasAuthorship W4308295089A5064478014 @default.
• W4308295089 hasAuthorship W4308295089A5068899977 @default.
• W4308295089 hasAuthorship W4308295089A5070622545 @default.
• W4308295089 hasAuthorship W4308295089A5071842934 @default.
• W4308295089 hasAuthorship W4308295089A5089109826 @default.
• W4308295089 hasBestOaLocation W43082950891 @default.
• W4308295089 hasConcept C102230213 @default.
• W4308295089 hasConcept C104317684 @default.
• W4308295089 hasConcept C122172344 @default.
• W4308295089 hasConcept C132455925 @default.
• W4308295089 hasConcept C141231307 @default.
• W4308295089 hasConcept C144501496 @default.
• W4308295089 hasConcept C189819185 @default.
• W4308295089 hasConcept C54355233 @default.
• W4308295089 hasConcept C70721500 @default.
• W4308295089 hasConcept C86803240 @default.
• W4308295089 hasConcept C98108389 @default.
• W4308295089 hasConceptScore W4308295089C102230213 @default.
• W4308295089 hasConceptScore W4308295089C104317684 @default.
• W4308295089 hasConceptScore W4308295089C122172344 @default.
• W4308295089 hasConceptScore W4308295089C132455925 @default.
• W4308295089 hasConceptScore W4308295089C141231307 @default.
• W4308295089 hasConceptScore W4308295089C144501496 @default.
• W4308295089 hasConceptScore W4308295089C189819185 @default.
• W4308295089 hasConceptScore W4308295089C54355233 @default.
• W4308295089 hasConceptScore W4308295089C70721500 @default.
• W4308295089 hasConceptScore W4308295089C86803240 @default.
• W4308295089 hasConceptScore W4308295089C98108389 @default.
• W4308295089 hasLocation W43082950891 @default.
• W4308295089 hasLocation W43082950892 @default.
• W4308295089 hasOpenAccess W4308295089 @default.

• next