FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308307842> ?p ?o ?g. }

• W4308307842 endingPage "66" @default.
• W4308307842 startingPage "35" @default.
• W4308307842 abstract "All epithelia have their basal side in contact with a specialized extracellular matrix, the basement membrane (BM). During development, the BM contributes to the shaping of epithelial organs via its mechanical properties. These properties rely on two core components of the BM, collagen type IV and perlecan/HSPG2, which both interact with another core component, laminin, the initiator of BM assembly. While collagen type IV supplies the BM with rigidity to constrain the tissue, perlecan antagonizes this effect. Nevertheless, the number of organs that has been studied is still scarce, and given that epithelial tissues exhibit a wide array of shapes, their forms are bound to be regulated by distinct mechanisms. This is underscored by mounting evidence that BM composition and assembly/biogenesis is tissue-specific. Moreover, previous reports have essentially focused on the mechanical role of the BM in morphogenesis at the tissue scale, but not the cell scale. Here, we took advantage of the robust conservation of core BM proteins and the limited genetic redundancy of the Drosophila model system to address how this matrix shapes the wing imaginal disc, a complex organ comprising a squamous, a cuboidal and a columnar epithelium. With the use of a hypomorphic allele, we show that the depletion of Trol (Drosophila perlecan) affects the morphogenesis of the three epithelia, but particularly that of the squamous one. The planar surface of the squamous epithelium (SE) becomes extremely narrow, due to a function for Trol in the control of the squamous shape of its cells. Furthermore, we find that the lack of Trol impairs the biogenesis of the BM of the SE by modifying the structure of the collagen type IV lattice. Through atomic force microscopy and laser surgery, we demonstrate that Trol provides elasticity to the SE's BM, thereby regulating the mechanical properties of the SE. Moreover, we show that Trol acts via collagen type IV, since the global reduction in the trol mutant context of collagen type IV or the enzyme that cross-links its 7S -but not the enzyme that cross-links its NC1- domain substantially restores the morphogenesis of the SE. In addition, a stronger decrease in collagen type IV achieved by the overexpression of the matrix metalloprotease 2 exclusively in the BM of the SE, significantly rescues the organization of the two other epithelia. Our data thus sustain a model in which Trol counters the rigidity conveyed by collagen type IV to the BM of the SE, via the regulation of the NC1-dependant assembly of its scaffold, allowing the spreading of the squamous cells, spreading which is compulsory for the architecture of the whole organ." @default.
• W4308307842 created "2022-11-10" @default.
• W4308307842 creator A5010369436 @default.
• W4308307842 creator A5018345126 @default.
• W4308307842 creator A5027255832 @default.
• W4308307842 creator A5038534132 @default.
• W4308307842 creator A5045280718 @default.
• W4308307842 creator A5046488783 @default.
• W4308307842 creator A5054374094 @default.
• W4308307842 creator A5064494082 @default.
• W4308307842 creator A5078158101 @default.
• W4308307842 date "2022-12-01" @default.
• W4308307842 modified "2023-09-25" @default.
• W4308307842 title "Regulation of the collagen IV network by the basement membrane protein perlecan is crucial for squamous epithelial cell morphogenesis and organ architecture" @default.
• W4308307842 cites W1460149411 @default.
• W4308307842 cites W1479893041 @default.
• W4308307842 cites W1548597922 @default.
• W4308307842 cites W1573495239 @default.
• W4308307842 cites W1618500214 @default.
• W4308307842 cites W1963828514 @default.
• W4308307842 cites W1964251746 @default.
• W4308307842 cites W1964521956 @default.
• W4308307842 cites W1967517363 @default.
• W4308307842 cites W1973976569 @default.
• W4308307842 cites W1976115473 @default.
• W4308307842 cites W1980739710 @default.
• W4308307842 cites W1981004291 @default.
• W4308307842 cites W1988533881 @default.
• W4308307842 cites W1992507751 @default.
• W4308307842 cites W2000671199 @default.
• W4308307842 cites W2002371107 @default.
• W4308307842 cites W2008839303 @default.
• W4308307842 cites W2018323091 @default.
• W4308307842 cites W2019650428 @default.
• W4308307842 cites W2036947524 @default.
• W4308307842 cites W2037076508 @default.
• W4308307842 cites W2041660617 @default.
• W4308307842 cites W2047721737 @default.
• W4308307842 cites W2048927510 @default.
• W4308307842 cites W2051630073 @default.
• W4308307842 cites W2052036630 @default.
• W4308307842 cites W2055018696 @default.
• W4308307842 cites W2055944257 @default.
• W4308307842 cites W2057551553 @default.
• W4308307842 cites W2061413060 @default.
• W4308307842 cites W2062336936 @default.
• W4308307842 cites W2072099999 @default.
• W4308307842 cites W2079868225 @default.
• W4308307842 cites W2080324470 @default.
• W4308307842 cites W2081781085 @default.
• W4308307842 cites W2084271768 @default.
• W4308307842 cites W2086419330 @default.
• W4308307842 cites W2089486085 @default.
• W4308307842 cites W2089818394 @default.
• W4308307842 cites W2090817340 @default.
• W4308307842 cites W2091884095 @default.
• W4308307842 cites W2093746797 @default.
• W4308307842 cites W2107819345 @default.
• W4308307842 cites W2110188006 @default.
• W4308307842 cites W2113058640 @default.
• W4308307842 cites W2116306687 @default.
• W4308307842 cites W2126747820 @default.
• W4308307842 cites W2128147740 @default.
• W4308307842 cites W2130093537 @default.
• W4308307842 cites W2139421867 @default.
• W4308307842 cites W2140377042 @default.
• W4308307842 cites W2140877665 @default.
• W4308307842 cites W2143156113 @default.
• W4308307842 cites W2144328981 @default.
• W4308307842 cites W2145033373 @default.
• W4308307842 cites W2145339827 @default.
• W4308307842 cites W2150015205 @default.
• W4308307842 cites W2159952016 @default.
• W4308307842 cites W2160427067 @default.
• W4308307842 cites W2163375761 @default.
• W4308307842 cites W2163416267 @default.
• W4308307842 cites W2167110071 @default.
• W4308307842 cites W2167807799 @default.
• W4308307842 cites W2237117893 @default.
• W4308307842 cites W2256212941 @default.
• W4308307842 cites W2472968686 @default.
• W4308307842 cites W2505336870 @default.
• W4308307842 cites W2511273639 @default.
• W4308307842 cites W2530992238 @default.
• W4308307842 cites W2533312017 @default.
• W4308307842 cites W2549070824 @default.
• W4308307842 cites W2567824251 @default.
• W4308307842 cites W2607477640 @default.
• W4308307842 cites W2727320713 @default.
• W4308307842 cites W2735307412 @default.
• W4308307842 cites W2750481248 @default.
• W4308307842 cites W2765175699 @default.
• W4308307842 cites W2765453603 @default.
• W4308307842 cites W2771385781 @default.
• W4308307842 cites W2794919347 @default.
• W4308307842 cites W2800869306 @default.
• W4308307842 cites W2892809983 @default.
• W4308307842 cites W2892850530 @default.

• next