Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308334868> ?p ?o ?g. }
- W4308334868 abstract "Actin is a multifunctional biomolecule that forms not only basic structural bodies such as filopodia and lamellipodia, but also large microvilli-like organelles like stereocilia. Actin consists of four sub-domains (S1, S2, S3, and S4), and the target-binding groove formed between S1 and S3 is the major binding site for various actin binding proteins. Actin filament dynamics are regulated by numerous actin binding proteins with different mechanisms of actin binding, assembly, and disassembly such as actin severing, branching, and bundling. Ectoplasmic specialization protein 1 (espin 1) is an actin binding and bundling protein that is specifically implicated in the elongation and stabilization of stereocilia as a binding partner with myosin III. However, little is known about the molecular structure, actin bundling, and stabilizing mechanism of espin 1; hence, we investigated the interaction between actin and espin 1 through structural data. In this study, we first purified human espin 1 in an E. coli system following a new detergent-free approach and then demonstrated the 2D structure of full-length espin 1 using transmission electron microscopy along with Nickel nitrilotriacetic acid nanogold labeling and 2D averaging using SPIDER. Furthermore, we also determined the espin 1 binding domain of actin using a co-sedimentation assay along with gelsolin and myosin S1. These findings are not only beneficial for understanding the actin binding and bundling mechanism of espin 1, but also shed light on its elongation, stabilization, and tip-localization mechanisms with myosin III. This study thus provides a basis for understanding the molecular structure of espin 1 and can contribute to various hearing-related diseases, such as hearing loss and vestibular dysfunction." @default.
- W4308334868 created "2022-11-11" @default.
- W4308334868 creator A5004078289 @default.
- W4308334868 creator A5027876191 @default.
- W4308334868 creator A5032178054 @default.
- W4308334868 creator A5050008313 @default.
- W4308334868 creator A5081878803 @default.
- W4308334868 date "2022-11-04" @default.
- W4308334868 modified "2023-09-30" @default.
- W4308334868 title "Studies of functional properties of espin 1: Its interaction to actin filaments" @default.
- W4308334868 cites W104354955 @default.
- W4308334868 cites W1626375823 @default.
- W4308334868 cites W1887123708 @default.
- W4308334868 cites W1982755401 @default.
- W4308334868 cites W1983869239 @default.
- W4308334868 cites W1989108940 @default.
- W4308334868 cites W2008974896 @default.
- W4308334868 cites W2010262553 @default.
- W4308334868 cites W2010744273 @default.
- W4308334868 cites W2012688036 @default.
- W4308334868 cites W2023474594 @default.
- W4308334868 cites W2025404281 @default.
- W4308334868 cites W2040899301 @default.
- W4308334868 cites W2048982317 @default.
- W4308334868 cites W2061230511 @default.
- W4308334868 cites W2062414231 @default.
- W4308334868 cites W2064066079 @default.
- W4308334868 cites W2068465317 @default.
- W4308334868 cites W2076123445 @default.
- W4308334868 cites W2107336909 @default.
- W4308334868 cites W2108372612 @default.
- W4308334868 cites W2110570424 @default.
- W4308334868 cites W2121858951 @default.
- W4308334868 cites W2122163415 @default.
- W4308334868 cites W2128463171 @default.
- W4308334868 cites W2167022381 @default.
- W4308334868 cites W2168446247 @default.
- W4308334868 cites W2168586729 @default.
- W4308334868 cites W2298485028 @default.
- W4308334868 cites W2515418674 @default.
- W4308334868 cites W2726683584 @default.
- W4308334868 cites W3008295843 @default.
- W4308334868 cites W51164494 @default.
- W4308334868 doi "https://doi.org/10.3389/fcell.2022.1022096" @default.
- W4308334868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36407097" @default.
- W4308334868 hasPublicationYear "2022" @default.
- W4308334868 type Work @default.
- W4308334868 citedByCount "0" @default.
- W4308334868 crossrefType "journal-article" @default.
- W4308334868 hasAuthorship W4308334868A5004078289 @default.
- W4308334868 hasAuthorship W4308334868A5027876191 @default.
- W4308334868 hasAuthorship W4308334868A5032178054 @default.
- W4308334868 hasAuthorship W4308334868A5050008313 @default.
- W4308334868 hasAuthorship W4308334868A5081878803 @default.
- W4308334868 hasBestOaLocation W43083348681 @default.
- W4308334868 hasConcept C109185818 @default.
- W4308334868 hasConcept C12554922 @default.
- W4308334868 hasConcept C125705527 @default.
- W4308334868 hasConcept C130861313 @default.
- W4308334868 hasConcept C142669718 @default.
- W4308334868 hasConcept C1491633281 @default.
- W4308334868 hasConcept C185592680 @default.
- W4308334868 hasConcept C2993400109 @default.
- W4308334868 hasConcept C3620293 @default.
- W4308334868 hasConcept C55493867 @default.
- W4308334868 hasConcept C6997183 @default.
- W4308334868 hasConcept C80722340 @default.
- W4308334868 hasConcept C86803240 @default.
- W4308334868 hasConcept C90301020 @default.
- W4308334868 hasConcept C95444343 @default.
- W4308334868 hasConceptScore W4308334868C109185818 @default.
- W4308334868 hasConceptScore W4308334868C12554922 @default.
- W4308334868 hasConceptScore W4308334868C125705527 @default.
- W4308334868 hasConceptScore W4308334868C130861313 @default.
- W4308334868 hasConceptScore W4308334868C142669718 @default.
- W4308334868 hasConceptScore W4308334868C1491633281 @default.
- W4308334868 hasConceptScore W4308334868C185592680 @default.
- W4308334868 hasConceptScore W4308334868C2993400109 @default.
- W4308334868 hasConceptScore W4308334868C3620293 @default.
- W4308334868 hasConceptScore W4308334868C55493867 @default.
- W4308334868 hasConceptScore W4308334868C6997183 @default.
- W4308334868 hasConceptScore W4308334868C80722340 @default.
- W4308334868 hasConceptScore W4308334868C86803240 @default.
- W4308334868 hasConceptScore W4308334868C90301020 @default.
- W4308334868 hasConceptScore W4308334868C95444343 @default.
- W4308334868 hasFunder F4320322120 @default.
- W4308334868 hasLocation W43083348681 @default.
- W4308334868 hasLocation W43083348682 @default.
- W4308334868 hasLocation W43083348683 @default.
- W4308334868 hasLocation W43083348684 @default.
- W4308334868 hasOpenAccess W4308334868 @default.
- W4308334868 hasPrimaryLocation W43083348681 @default.
- W4308334868 hasRelatedWork W2018901145 @default.
- W4308334868 hasRelatedWork W2053917765 @default.
- W4308334868 hasRelatedWork W2081485263 @default.
- W4308334868 hasRelatedWork W2103431046 @default.
- W4308334868 hasRelatedWork W2145357604 @default.
- W4308334868 hasRelatedWork W2513116541 @default.
- W4308334868 hasRelatedWork W2530712933 @default.
- W4308334868 hasRelatedWork W2655146467 @default.