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• W4308337364 abstract "<ns4:p><ns4:bold>Background: </ns4:bold>Patients with primary sclerosing cholangitis (PSC) frequently have co-ocurring ulcerative colitis (UC) and develop colorectal cancer. Colorectal cancer risk in patients with PSC-associated ulcerative colitis (PSC/UC) is elevated relative to patients with ulcerative colitis (UC) alone, reasons for which remain obscure. Understanding the molecular and microbial basis for differences between these two patient groups and how these vary across intestinal sites is important for the development of therapies to prevent colorectal cancer development in at-risk individuals. </ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>We employed ribonucleic acid sequencing (RNA-seq) analysis of biopsy samples across three intestinal tissue locations (ileum, caecum and rectum) in patients with PSC/UC (ileum n = 7, caecum n = 7, rectum n = 7), UC (ileum n = 9, caecum n = 10, rectum n = 10) and healthy controls (ileum n = 11, caecum n = 9, rectum n = 12) to determine tissue-dependent transcriptional alterations in PSC/UC. We also performed 16S ribosomal RNA (rRNA) amplicon sequencing to determine bacterial associations with PSC/UC.</ns4:p><ns4:p> <ns4:bold>Results: </ns4:bold>Tissue-defining transcriptional signatures revealed that the ileum was enriched for genes involved in lipid and drug metabolism, the caecum for activated immune cells and the rectum for enteric neurogenesis. Transcriptional alterations relative to healthy control samples were largely shared between patients with PSC/UC or UC although were distinct across tissue locations. Nevertheless, we observed reduced expression of gamma-glutamyl transferase 1 (<ns4:italic>GGT1</ns4:italic>) specifically in the ileum and caecum of patients with PSC/UC.</ns4:p><ns4:p> Analysis of the bacterial component of the microbiome revealed high inter-individual variability of microbiome composition and little evidence for tissue-dependency. We observed a reduction in <ns4:italic>Parabacteroides</ns4:italic> relative abundance in the rectum of patients with PSC/UC.</ns4:p><ns4:p> <ns4:bold>Conclusions: </ns4:bold>The role of gamma-glutamyl transferase in maintaining the redox environment through the glutathione salvage pathway makes our observed alterations a potential pathway to PSC-associated colorectal cancer.</ns4:p>" @default.
• W4308337364 created "2022-11-11" @default.
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• W4308337364 date "2022-11-04" @default.
• W4308337364 modified "2023-09-27" @default.
• W4308337364 title "Tissue-dependent transcriptional and bacterial associations in primary sclerosing cholangitis-associated inflammatory bowel disease" @default.
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• W4308337364 doi "https://doi.org/10.12688/wellcomeopenres.16901.2" @default.
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