Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308347307> ?p ?o ?g. }
- W4308347307 endingPage "105277" @default.
- W4308347307 startingPage "105277" @default.
- W4308347307 abstract "The Varroa mite, Varroa destructor, poses one of the most serious threats to honey bees worldwide. Although coumaphos, an anticholinesterase pesticide, is widely used for varroa mite control, little information is available on the properties of Varroa mite acetylcholinesterases (VdAChEs). In this study, three putative VdAChEs were annotated and named VdAChE1, VdAChE2, and VdAChE3. All VdAChEs possessed most of the functionally important signature domains, suggesting that they are catalytically active. Phylogenetic analysis revealed that VdAChE1 was clustered into a clade containing most arthropod AChE1s, whereas VdAChE2 and VdAChE3 formed a unique clade with other arachnid AChEs. VdAChE1 was determined to be membrane-anchored, but both VdAChE2 and VdAChE3 are soluble, as judged by electrophoresis in conjunction with western blotting. Tissue-specific transcription profiling revealed that VdAChE1 was most predominantly expressed in the synganglion. In contrast, VdAChE2 was most predominantly expressed in the legs and cuticle. VdAChE3 showed negligible expression levels in all the tissues examined. In a kinetic analysis using recombinant VdAChEs, VdAChE1 exhibited the highest catalytic efficiency, followed by VdAChE2 and VdAChE3. Inhibition experiments revealed that VdAChE1 was most sensitive to all tested inhibitors. Taken together, VdAChE1 appears to be the major synaptic enzyme with a more toxicological relevance, whereas VdAChE2 is involved in other noncatalytic functions, including chemical defense against xenobiotics. Current findings contribute to a more detailed understanding of the evolutionary and functional traits of VdAChEs and to the design of novel anticholinesterase varroacides." @default.
- W4308347307 created "2022-11-11" @default.
- W4308347307 creator A5005487766 @default.
- W4308347307 creator A5012581232 @default.
- W4308347307 creator A5017338587 @default.
- W4308347307 creator A5036362321 @default.
- W4308347307 creator A5050016859 @default.
- W4308347307 creator A5084977213 @default.
- W4308347307 date "2022-11-01" @default.
- W4308347307 modified "2023-10-16" @default.
- W4308347307 title "Molecular and kinetic properties of three acetylcholinesterases in the Varroa mite, Varroa destructor" @default.
- W4308347307 cites W1562398969 @default.
- W4308347307 cites W1619964209 @default.
- W4308347307 cites W1964394467 @default.
- W4308347307 cites W1965923040 @default.
- W4308347307 cites W1966468993 @default.
- W4308347307 cites W1975073337 @default.
- W4308347307 cites W1975899714 @default.
- W4308347307 cites W1986232872 @default.
- W4308347307 cites W1987336494 @default.
- W4308347307 cites W1992647991 @default.
- W4308347307 cites W2002295022 @default.
- W4308347307 cites W2013132270 @default.
- W4308347307 cites W2015331537 @default.
- W4308347307 cites W2023923301 @default.
- W4308347307 cites W2026523721 @default.
- W4308347307 cites W2035043090 @default.
- W4308347307 cites W2038231696 @default.
- W4308347307 cites W2044658037 @default.
- W4308347307 cites W2045031208 @default.
- W4308347307 cites W2059454104 @default.
- W4308347307 cites W2072192337 @default.
- W4308347307 cites W2095001237 @default.
- W4308347307 cites W2108244474 @default.
- W4308347307 cites W2141816565 @default.
- W4308347307 cites W2149098307 @default.
- W4308347307 cites W2158984087 @default.
- W4308347307 cites W2176737928 @default.
- W4308347307 cites W2464420150 @default.
- W4308347307 cites W2471684930 @default.
- W4308347307 cites W2597504826 @default.
- W4308347307 cites W2770368987 @default.
- W4308347307 cites W2793158785 @default.
- W4308347307 cites W2799524357 @default.
- W4308347307 cites W2894039806 @default.
- W4308347307 cites W2910400461 @default.
- W4308347307 cites W3180039384 @default.
- W4308347307 cites W4206220303 @default.
- W4308347307 cites W4281751147 @default.
- W4308347307 cites W925450048 @default.
- W4308347307 doi "https://doi.org/10.1016/j.pestbp.2022.105277" @default.
- W4308347307 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36464382" @default.
- W4308347307 hasPublicationYear "2022" @default.
- W4308347307 type Work @default.
- W4308347307 citedByCount "3" @default.
- W4308347307 countsByYear W43083473072023 @default.
- W4308347307 crossrefType "journal-article" @default.
- W4308347307 hasAuthorship W4308347307A5005487766 @default.
- W4308347307 hasAuthorship W4308347307A5012581232 @default.
- W4308347307 hasAuthorship W4308347307A5017338587 @default.
- W4308347307 hasAuthorship W4308347307A5036362321 @default.
- W4308347307 hasAuthorship W4308347307A5050016859 @default.
- W4308347307 hasAuthorship W4308347307A5084977213 @default.
- W4308347307 hasConcept C2777907900 @default.
- W4308347307 hasConcept C2779381614 @default.
- W4308347307 hasConcept C2779711295 @default.
- W4308347307 hasConcept C2780871575 @default.
- W4308347307 hasConcept C33070731 @default.
- W4308347307 hasConcept C54355233 @default.
- W4308347307 hasConcept C59822182 @default.
- W4308347307 hasConcept C74714110 @default.
- W4308347307 hasConcept C86803240 @default.
- W4308347307 hasConcept C90856448 @default.
- W4308347307 hasConceptScore W4308347307C2777907900 @default.
- W4308347307 hasConceptScore W4308347307C2779381614 @default.
- W4308347307 hasConceptScore W4308347307C2779711295 @default.
- W4308347307 hasConceptScore W4308347307C2780871575 @default.
- W4308347307 hasConceptScore W4308347307C33070731 @default.
- W4308347307 hasConceptScore W4308347307C54355233 @default.
- W4308347307 hasConceptScore W4308347307C59822182 @default.
- W4308347307 hasConceptScore W4308347307C74714110 @default.
- W4308347307 hasConceptScore W4308347307C86803240 @default.
- W4308347307 hasConceptScore W4308347307C90856448 @default.
- W4308347307 hasFunder F4320322035 @default.
- W4308347307 hasLocation W43083473071 @default.
- W4308347307 hasLocation W43083473072 @default.
- W4308347307 hasOpenAccess W4308347307 @default.
- W4308347307 hasPrimaryLocation W43083473071 @default.
- W4308347307 hasRelatedWork W1907040463 @default.
- W4308347307 hasRelatedWork W1977404635 @default.
- W4308347307 hasRelatedWork W2099800103 @default.
- W4308347307 hasRelatedWork W2571654480 @default.
- W4308347307 hasRelatedWork W2896918368 @default.
- W4308347307 hasRelatedWork W2996616411 @default.
- W4308347307 hasRelatedWork W3111388645 @default.
- W4308347307 hasRelatedWork W3158828885 @default.
- W4308347307 hasRelatedWork W3196584538 @default.
- W4308347307 hasRelatedWork W3201505630 @default.