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- W4308357925 abstract "Advances in sequencing technologies have produced a huge amount of transcriptome data, which facilitate the revelation of natural product biosynthetic pathways in plants. Asiaticoside and madecassoside are two famous triterpene saponins with unusual glucose-glucose-rhamnose chain at the C-28 position of the aglycone. To date, the complete glycosylation pathway underlying the assembly of the above sugar chain remains largely unknown. Here we successfully screened out 75 putative uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs) by analyzing the latest transcriptome data of Centella asiatica. We further identified a UGT (CaUGT1) that could specifically transfer the glucose to the C-28 carboxyl group of asiatic acid and madecassic acid. Interestingly, the expression profile of CaUGT1 is highly correlated with UGT73AD1, a previously characterized UDP-glucose 28-O-glucosyltransferase, suggesting that the initial glycosylation step of asiatic acid and madecassic acid was synergistically catalyzed by these two UGTs. This study thus laid the foundation for a comprehensively understanding of the glycosylation network in the biosynthesis of asiaticoside and madecassoside." @default.
- W4308357925 created "2022-11-11" @default.
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- W4308357925 date "2022-12-01" @default.
- W4308357925 modified "2023-10-16" @default.
- W4308357925 title "Revisiting the transcriptome data of Centella asiatica identified an ester-forming triterpenoid: UDP-glucose 28-O-glucosyltransferase" @default.
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- W4308357925 doi "https://doi.org/10.1016/j.tet.2022.133136" @default.
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