Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308367072> ?p ?o ?g. }
- W4308367072 endingPage "786" @default.
- W4308367072 startingPage "777" @default.
- W4308367072 abstract "To test the hypothesis that reseeded anterior cruciate ligament (ACL)-derived cells have a better ability to survive and integrate into tendon extracellular matrix (ECM) and accelerate the ligamentization process, compared to adipose-derived mesenchymal stem cells (ADMSCs).Acellularized tibialis allograft tendons were used. Tendons were randomly reseeded with ACL-derived cells or ADMSCs. ACL-derived cells were harvested and isolated from remnants of ruptured ACLs during reconstruction surgery and cultured at passage three. Cell suspensions (200 µl) containing 2 × 106 ACL-derived cells or ADMSCs were prepared for the purpose of reseeding. At days 1, 3, and 7 post-reseeding, graft composites were assessed for repopulation with histological and immunohistochemical analysis. Matrix protein contents and gene expression levels were analyzed.In the graft reseeded with ACL-derived cells, a large number of elongated cells that integrated into the matrix were evident at day 3 and day 7. However, in the graft reseeded with ADMSCs, only a small number of elongated cells were found integrated into the matrix. Immunofluorescence for Ki-67 and type I collagen confirmed the pronounced production of type I collagen by Ki-67-positive ACL-derived cells integrated into the ECM. A messenger RNA (mRNA) expression assay demonstrated significantly higher gene expression levels of types I (p = 0.013) and III (p = 0.050) collagen in the composites reseeded with ACL-derived cells than ADMSCs.ACL-derived cells, when reseeded to acellularized tendon graft, demonstrated earlier better survival and integration in the tendon ECM and resulted in higher gene expression levels of collagen, which may be essential to the normal ligamentization process compared to ADMSCs.Cite this article: Bone Joint Res 2022;11(11):777-786." @default.
- W4308367072 created "2022-11-11" @default.
- W4308367072 creator A5022748342 @default.
- W4308367072 creator A5026216541 @default.
- W4308367072 creator A5039398861 @default.
- W4308367072 creator A5055277661 @default.
- W4308367072 creator A5063577300 @default.
- W4308367072 creator A5084287451 @default.
- W4308367072 date "2022-11-01" @default.
- W4308367072 modified "2023-10-01" @default.
- W4308367072 title "Comparison of ligamentization potential between anterior cruciate ligament-derived cells and adipose-derived mesenchymal stem cells reseeded to acellularized tendon allograft" @default.
- W4308367072 cites W1589631482 @default.
- W4308367072 cites W1912584323 @default.
- W4308367072 cites W1921978279 @default.
- W4308367072 cites W1965396889 @default.
- W4308367072 cites W1982024058 @default.
- W4308367072 cites W198810802 @default.
- W4308367072 cites W1990419270 @default.
- W4308367072 cites W1993080116 @default.
- W4308367072 cites W2012892026 @default.
- W4308367072 cites W2015050458 @default.
- W4308367072 cites W2018028722 @default.
- W4308367072 cites W2021789319 @default.
- W4308367072 cites W2023416936 @default.
- W4308367072 cites W2027911167 @default.
- W4308367072 cites W2030176917 @default.
- W4308367072 cites W2030560139 @default.
- W4308367072 cites W2031820224 @default.
- W4308367072 cites W2034887920 @default.
- W4308367072 cites W2041987582 @default.
- W4308367072 cites W2045329465 @default.
- W4308367072 cites W2073968937 @default.
- W4308367072 cites W2075235098 @default.
- W4308367072 cites W2090947054 @default.
- W4308367072 cites W2091151986 @default.
- W4308367072 cites W2100140518 @default.
- W4308367072 cites W2105203266 @default.
- W4308367072 cites W2130441881 @default.
- W4308367072 cites W2131605870 @default.
- W4308367072 cites W2136273741 @default.
- W4308367072 cites W2136842127 @default.
- W4308367072 cites W2143110295 @default.
- W4308367072 cites W2144757775 @default.
- W4308367072 cites W2171531663 @default.
- W4308367072 cites W2232494399 @default.
- W4308367072 cites W2602561858 @default.
- W4308367072 cites W2606215056 @default.
- W4308367072 cites W2612488077 @default.
- W4308367072 cites W2807518326 @default.
- W4308367072 cites W2889705240 @default.
- W4308367072 cites W2901613071 @default.
- W4308367072 cites W2917177558 @default.
- W4308367072 cites W2954392401 @default.
- W4308367072 cites W2954807210 @default.
- W4308367072 cites W3008413096 @default.
- W4308367072 cites W3013807418 @default.
- W4308367072 cites W3036719365 @default.
- W4308367072 cites W3047821595 @default.
- W4308367072 cites W3082692759 @default.
- W4308367072 cites W3149079793 @default.
- W4308367072 cites W3163653761 @default.
- W4308367072 cites W3203078833 @default.
- W4308367072 doi "https://doi.org/10.1302/2046-3758.1111.bjr-2021-0548.r2" @default.
- W4308367072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36342052" @default.
- W4308367072 hasPublicationYear "2022" @default.
- W4308367072 type Work @default.
- W4308367072 citedByCount "2" @default.
- W4308367072 countsByYear W43083670722023 @default.
- W4308367072 crossrefType "journal-article" @default.
- W4308367072 hasAuthorship W4308367072A5022748342 @default.
- W4308367072 hasAuthorship W4308367072A5026216541 @default.
- W4308367072 hasAuthorship W4308367072A5039398861 @default.
- W4308367072 hasAuthorship W4308367072A5055277661 @default.
- W4308367072 hasAuthorship W4308367072A5063577300 @default.
- W4308367072 hasAuthorship W4308367072A5084287451 @default.
- W4308367072 hasBestOaLocation W43083670721 @default.
- W4308367072 hasConcept C105702510 @default.
- W4308367072 hasConcept C106487976 @default.
- W4308367072 hasConcept C126322002 @default.
- W4308367072 hasConcept C136229726 @default.
- W4308367072 hasConcept C142724271 @default.
- W4308367072 hasConcept C16685009 @default.
- W4308367072 hasConcept C171089720 @default.
- W4308367072 hasConcept C185592680 @default.
- W4308367072 hasConcept C189165786 @default.
- W4308367072 hasConcept C198826908 @default.
- W4308367072 hasConcept C2778434673 @default.
- W4308367072 hasConcept C2780105995 @default.
- W4308367072 hasConcept C28328180 @default.
- W4308367072 hasConcept C43617362 @default.
- W4308367072 hasConcept C49892992 @default.
- W4308367072 hasConcept C71924100 @default.
- W4308367072 hasConcept C86803240 @default.
- W4308367072 hasConcept C95444343 @default.
- W4308367072 hasConceptScore W4308367072C105702510 @default.
- W4308367072 hasConceptScore W4308367072C106487976 @default.
- W4308367072 hasConceptScore W4308367072C126322002 @default.
- W4308367072 hasConceptScore W4308367072C136229726 @default.