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- W4308367141 abstract "Cell-free DNA (cfDNA) is of growing clinical and research significance. In vitro cfDNA models are a useful tool in cfDNA research; however, artifacts in these models may have implications for the interpretation of new and published data. This report aimed to establish how endogenous cfDNA in fetal bovine serum (FBS) may influence in vitro cfDNA measurements. Three commercial cell culture media, supplemented with 10% FBS, were analyzed for the presence of cfDNA, with and without culture with ovarian cancer cell lines. cfDNA from FBS was identified with all three commercial media and contributed a major portion of 167-bp cfDNA. Future studies should account for bovine cfDNA in FBS-supplemented media when conducting in vitro cfDNA research." @default.
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- W4308367141 date "2022-11-01" @default.
- W4308367141 modified "2023-09-30" @default.
- W4308367141 title "Endogenous cell-free DNA in fetal bovine serum introduces artifacts to <i>in vitro</i> cell-free DNA models" @default.
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- W4308367141 doi "https://doi.org/10.2144/btn-2022-0040" @default.
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