FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308372272> ?p ?o ?g. }

• W4308372272 endingPage "170" @default.
• W4308372272 startingPage "159" @default.
• W4308372272 abstract "dmrtb1 performs critical functions in sex determination/differentiation and gonadal development in many organisms, but its role in teleost is rarely studied. Through gene cloning, in situ hybridization, and RNA interference technology, the function of dmrtb1 in testicular development of large yellow croaker (Larimichthys crocea) was studied; our study will be helpful in understanding further the molecular regulation mechanism of Lcdmrtb1/Lcdmrt6 in testicular development in L. crocea, and our results enrich the theory of fish dmrts involved in reproductive regulation and provide a new idea for sex control breeding of L. crocea by manipulating reproductive pathway.Doublesex- and mab-3-related transcription factor B1 (dmrtb1/dmrt6) belongs to one of the members of DMRT family, which performs critical functions in sex determination and differentiation, gonadal development, and functional maintenance. However, knowledge of its exact mechanism remains unclear in teleost. Very little is known about the role of dmrtb1 in the gonad development of Larimichthys crocea. In this study, a dmrtb1 homolog in L. crocea named as Lcdmrtb1 with the full-length cDNA was isolated and characterized. Except for the conserved DM domain, the other regions had low homology. Of the tissues sampled, Lcdmrtb1 was only found to be highly expressed in the testis. In situ hybridization of testis revealed Lcdmrtb1 in both spermatogonia and spermatocytes. After Lcdmrtb1 interference in the testis cells (LYCT) of L. crocea, the expression levels of Lcdmrtb1 and Lcdmrt1 were significantly decreased; subsequently, testicular cell morphology changed from fibrous to round and their growth rate slowed. Similarly, the expression levels of Lcdmrtb1, Lcdmrt1, sox9a/b, and amh were significantly decreased after RNAi in the testis. Furthermore, it was discovered that the spermatogonia had disappeared, and the Sertoli cells had been reduced. The results of immunohistochemistry showed that the expression of Sox9 protein in the testis was not detected after dmrtb1 was knocked down. These results indicated that the absence of Lcdmrtb1 not only greatly inhibited cell growth and destroyed the morphology of testis cells but also down-regulated Lcdmrt1 expression in the testis. This study will be helpful in understanding further the molecular regulation mechanism of Lcdmrtb1/Lcdmrt6 in testicular development in L. crocea." @default.
• W4308372272 created "2022-11-11" @default.
• W4308372272 creator A5002965035 @default.
• W4308372272 creator A5029395219 @default.
• W4308372272 creator A5040053880 @default.
• W4308372272 creator A5054051854 @default.
• W4308372272 creator A5068139353 @default.
• W4308372272 creator A5073885433 @default.
• W4308372272 creator A5087261074 @default.
• W4308372272 creator A5089236349 @default.
• W4308372272 date "2023-02-01" @default.
• W4308372272 modified "2023-09-30" @default.
• W4308372272 title "dmrtb1 is involved in the testicular development in Larimichthys crocea" @default.
• W4308372272 cites W1569764381 @default.
• W4308372272 cites W1615728431 @default.
• W4308372272 cites W1859775981 @default.
• W4308372272 cites W1965762845 @default.
• W4308372272 cites W1988749905 @default.
• W4308372272 cites W2000566561 @default.
• W4308372272 cites W2006203743 @default.
• W4308372272 cites W2011581056 @default.
• W4308372272 cites W2028354234 @default.
• W4308372272 cites W2029854938 @default.
• W4308372272 cites W2036738539 @default.
• W4308372272 cites W2041784242 @default.
• W4308372272 cites W2060637170 @default.
• W4308372272 cites W2083959830 @default.
• W4308372272 cites W2084854441 @default.
• W4308372272 cites W2095284418 @default.
• W4308372272 cites W2102190537 @default.
• W4308372272 cites W2102277799 @default.
• W4308372272 cites W2127911142 @default.
• W4308372272 cites W2159597721 @default.
• W4308372272 cites W2159851983 @default.
• W4308372272 cites W2167072163 @default.
• W4308372272 cites W2170190703 @default.
• W4308372272 cites W2175262244 @default.
• W4308372272 cites W2626857064 @default.
• W4308372272 cites W2762244099 @default.
• W4308372272 cites W2772746814 @default.
• W4308372272 cites W2883204536 @default.
• W4308372272 cites W3042411372 @default.
• W4308372272 cites W3100738057 @default.
• W4308372272 doi "https://doi.org/10.1530/rep-22-0214" @default.
• W4308372272 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36342669" @default.
• W4308372272 hasPublicationYear "2023" @default.
• W4308372272 type Work @default.
• W4308372272 citedByCount "1" @default.
• W4308372272 countsByYear W43083722722023 @default.
• W4308372272 crossrefType "journal-article" @default.
• W4308372272 hasAuthorship W4308372272A5002965035 @default.
• W4308372272 hasAuthorship W4308372272A5029395219 @default.
• W4308372272 hasAuthorship W4308372272A5040053880 @default.
• W4308372272 hasAuthorship W4308372272A5054051854 @default.
• W4308372272 hasAuthorship W4308372272A5068139353 @default.
• W4308372272 hasAuthorship W4308372272A5073885433 @default.
• W4308372272 hasAuthorship W4308372272A5087261074 @default.
• W4308372272 hasAuthorship W4308372272A5089236349 @default.
• W4308372272 hasConcept C104317684 @default.
• W4308372272 hasConcept C121050878 @default.
• W4308372272 hasConcept C134018914 @default.
• W4308372272 hasConcept C137487509 @default.
• W4308372272 hasConcept C150194340 @default.
• W4308372272 hasConcept C165525559 @default.
• W4308372272 hasConcept C187882448 @default.
• W4308372272 hasConcept C199360897 @default.
• W4308372272 hasConcept C2776514529 @default.
• W4308372272 hasConcept C2781471917 @default.
• W4308372272 hasConcept C41008148 @default.
• W4308372272 hasConcept C54355233 @default.
• W4308372272 hasConcept C54458228 @default.
• W4308372272 hasConcept C63405245 @default.
• W4308372272 hasConcept C67705224 @default.
• W4308372272 hasConcept C81439078 @default.
• W4308372272 hasConcept C86803240 @default.
• W4308372272 hasConcept C95444343 @default.
• W4308372272 hasConceptScore W4308372272C104317684 @default.
• W4308372272 hasConceptScore W4308372272C121050878 @default.
• W4308372272 hasConceptScore W4308372272C134018914 @default.
• W4308372272 hasConceptScore W4308372272C137487509 @default.
• W4308372272 hasConceptScore W4308372272C150194340 @default.
• W4308372272 hasConceptScore W4308372272C165525559 @default.
• W4308372272 hasConceptScore W4308372272C187882448 @default.
• W4308372272 hasConceptScore W4308372272C199360897 @default.
• W4308372272 hasConceptScore W4308372272C2776514529 @default.
• W4308372272 hasConceptScore W4308372272C2781471917 @default.
• W4308372272 hasConceptScore W4308372272C41008148 @default.
• W4308372272 hasConceptScore W4308372272C54355233 @default.
• W4308372272 hasConceptScore W4308372272C54458228 @default.
• W4308372272 hasConceptScore W4308372272C63405245 @default.
• W4308372272 hasConceptScore W4308372272C67705224 @default.
• W4308372272 hasConceptScore W4308372272C81439078 @default.
• W4308372272 hasConceptScore W4308372272C86803240 @default.
• W4308372272 hasConceptScore W4308372272C95444343 @default.
• W4308372272 hasIssue "2" @default.
• W4308372272 hasLocation W43083722721 @default.
• W4308372272 hasLocation W43083722722 @default.
• W4308372272 hasOpenAccess W4308372272 @default.

• next