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- W4308378129 abstract "<h3>Background</h3> Chimeric antigen receptor (CAR) T-cells are engineered immune cells that can be taken from a patient and redirected to fight cancer. Recently, we developed a virus-free process to create anti-GD2 CAR T cells using CRISPR-Cas9 ribonucleoproteins that endows cells with a stem cell memory-like and less exhausted phenotype that improves their ability to induce regression of GD2 positive solid tumors. <h3>Methods</h3> To improve this workflow, we systematically modified the media conditions, electroporation timing, and small molecule treatment to improve the cell yield and memory-like characteristics of CAR T cells. <h3>Results</h3> We find that optimal non-viral knock-in is dependent on activation time, and two key small molecule inhibitors. <h3>Conclusions</h3> Key reagents have been made GMP-compatible, and therefore this process could be readily implemented in existing cell manufacturing facilities. <h3>Ethics Approval</h3> The study was approved by the IRB at University of Wisconsin-Madison, approval number 2018-0103" @default.
- W4308378129 created "2022-11-11" @default.
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- W4308378129 date "2022-11-01" @default.
- W4308378129 modified "2023-09-26" @default.
- W4308378129 title "391 Development of a GMP-compatible, virus-free CRISPR CAR T cell manufacturing process" @default.
- W4308378129 doi "https://doi.org/10.1136/jitc-2022-sitc2022.0391" @default.
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