FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308378500> ?p ?o ?g. }

• W4308378500 abstract "<h3>Background</h3> Adoptive T cell therapy is an important modality of cancer immunotherapy. Engineering of various genes associated with improved T cell function, such as viability, sustainability, migration potential, and target specificity is important. Therefore, we need to identify effective methods for multigene transfer to T cells. The selection of appropriate promoters or linkers in the production of virus vectors is critical for efficacy of multigene expression. <h3>Methods</h3> In order to optimize vectors that can increase the expression efficiency of multigenes, we intended to compare the expression amount of each gene with different promoters and linkers. The expression of each gene was compared when 1G4 TCR (alpha and beta chains are linked by furin-SGSG-P2A) and CCR10 were linked using IRES, T2A, or furin-SGSG-T2A. Three cloning vectors were produced using one EF1a, one EFS, or two EFS to express 1G4 and CCR10 genes simultaneously with T2A. Transfection was performed on Lenti-x 293T and transduction was performed in Jurkat cells to measure the difference in expression of the 1G4 with mouse TCR constant antibody and CCR10 protein by flow cytometry. Finally, 1G4 and CCR10 dual expressing cells were sorted and cultured to see the persistence of expression on the day of culture, day 6, and day 12. <h3>Results</h3> Since CCR10 was not expressed with the vector using IRES, T2A was selected. In addition, differences in protein expression between T2A and furin-SGSG-T2A were not observed. Using two EFS promotors, 1G4 and CCR10 were expressed at 4.3% and 33.4%, respectively, so they were not studied further. When using one EFS promotor, 1G4 was expressed at 97.6%, but CCR10 (downstream of T2A) was expressed significantly lower at 26.1% on the transduction day. When using the EF1a, the expression of 1G4 was decreased to 74.2%, but CCR10 expression was increased to 37.4%. After sorting of dual expressing cells, the expression of 1G4 and CCR10 with the EFS decreased from 100% and 86.1% (day 0) to 89.5% and 2.66%, respectively (day 12). However, with the EF1a, the expression of 1G4 was almost the same from 99.2% (day 0) to 99.7% (day 12), and CCR10 expression decreased slightly from 94.6% (day 0) to 73.5% (day 12). <h3>Conclusions</h3> T2A showed better gene expression efficiency for downstream gene than IRES. EF1a was better than EFS and it was associated durable transduction efficacy. Comparison of linkers and promoters are necessary to improve multigene transfer to T cells for adoptive T cell therapy." @default.
• W4308378500 created "2022-11-11" @default.
• W4308378500 creator A5005206772 @default.
• W4308378500 creator A5010518834 @default.
• W4308378500 creator A5032349144 @default.
• W4308378500 creator A5048995585 @default.
• W4308378500 creator A5051905445 @default.
• W4308378500 creator A5082406125 @default.
• W4308378500 date "2022-11-01" @default.
• W4308378500 modified "2023-09-25" @default.
• W4308378500 title "75 Methods to improve co-expression efficacy of genes modulating promoter and linker" @default.
• W4308378500 doi "https://doi.org/10.1136/jitc-2022-sitc2022.0075" @default.
• W4308378500 hasPublicationYear "2022" @default.
• W4308378500 type Work @default.
• W4308378500 citedByCount "0" @default.
• W4308378500 crossrefType "proceedings-article" @default.
• W4308378500 hasAuthorship W4308378500A5005206772 @default.
• W4308378500 hasAuthorship W4308378500A5010518834 @default.
• W4308378500 hasAuthorship W4308378500A5032349144 @default.
• W4308378500 hasAuthorship W4308378500A5048995585 @default.
• W4308378500 hasAuthorship W4308378500A5051905445 @default.
• W4308378500 hasAuthorship W4308378500A5082406125 @default.
• W4308378500 hasBestOaLocation W43083785001 @default.
• W4308378500 hasConcept C104317684 @default.
• W4308378500 hasConcept C15152581 @default.
• W4308378500 hasConcept C153911025 @default.
• W4308378500 hasConcept C181199279 @default.
• W4308378500 hasConcept C2777265534 @default.
• W4308378500 hasConcept C33161422 @default.
• W4308378500 hasConcept C40767141 @default.
• W4308378500 hasConcept C54009773 @default.
• W4308378500 hasConcept C54355233 @default.
• W4308378500 hasConcept C553184892 @default.
• W4308378500 hasConcept C55493867 @default.
• W4308378500 hasConcept C70721500 @default.
• W4308378500 hasConcept C86803240 @default.
• W4308378500 hasConcept C95444343 @default.
• W4308378500 hasConceptScore W4308378500C104317684 @default.
• W4308378500 hasConceptScore W4308378500C15152581 @default.
• W4308378500 hasConceptScore W4308378500C153911025 @default.
• W4308378500 hasConceptScore W4308378500C181199279 @default.
• W4308378500 hasConceptScore W4308378500C2777265534 @default.
• W4308378500 hasConceptScore W4308378500C33161422 @default.
• W4308378500 hasConceptScore W4308378500C40767141 @default.
• W4308378500 hasConceptScore W4308378500C54009773 @default.
• W4308378500 hasConceptScore W4308378500C54355233 @default.
• W4308378500 hasConceptScore W4308378500C553184892 @default.
• W4308378500 hasConceptScore W4308378500C55493867 @default.
• W4308378500 hasConceptScore W4308378500C70721500 @default.
• W4308378500 hasConceptScore W4308378500C86803240 @default.
• W4308378500 hasConceptScore W4308378500C95444343 @default.
• W4308378500 hasLocation W43083785001 @default.
• W4308378500 hasOpenAccess W4308378500 @default.
• W4308378500 hasPrimaryLocation W43083785001 @default.
• W4308378500 hasRelatedWork W2349850548 @default.
• W4308378500 hasRelatedWork W2353507437 @default.
• W4308378500 hasRelatedWork W2369377256 @default.
• W4308378500 hasRelatedWork W2372462472 @default.
• W4308378500 hasRelatedWork W2381551108 @default.
• W4308378500 hasRelatedWork W2386311237 @default.
• W4308378500 hasRelatedWork W2394365893 @default.
• W4308378500 hasRelatedWork W2401067645 @default.
• W4308378500 hasRelatedWork W2418333805 @default.
• W4308378500 hasRelatedWork W3029124564 @default.
• W4308378500 isParatext "false" @default.
• W4308378500 isRetracted "false" @default.
• W4308378500 workType "article" @default.