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- W4308457286 abstract "Abstract Background and Aims Recurrence is the major cause of poor prognosis in hepatocellular carcinoma (HCC), especially in those infected with HBV. Telomerase reverse transcriptase (TERT) promoter is the most prevalent site in HBV-related HCC. We attempted to evaluate the prognostic value of TERT mutation status in HBV-related HCC patients. Methods We enrolled the 66 HBV-related HCC patients who underwent hepatectomy between January 2013 and May 2016. DNA extracted from the fresh tumor tissues of these patients was subjected to Sanger sequencing and optimized droplet polymerase chain reaction (dPCR) for detecting TERT promoter mutation. We optimized the dPCR assay by adding 7-deaza-dGTP, CviQ1, and EDTA for improving the detection of TERT promoter mutation. Furthermore, nomogram survival prediction models were developed to assess the prognostic value of HBV-related HCC after hepatectomy. Results The frequency of TERT C228T mutation was detected 24/66 (36.36%) by Sanger sequencing and 30/66 (45.45%) by dPCR. The two methods had an initial overall agreement(concordance = 90.91%, kappa = 0.814; dPCR sensitivity, 100%; dPCR specificity, 85.71%). HBV-related HCC patients with TERT C228T mutation had reduced survival and increased risk of recurrence. TERT C228T was an independent prognostic factor for OS and DFS in multivariate analyses. The nomograms successfully differentiated TERT C228T mutant and non-mutant HBV-related HCC patients associated with OS and DFS (C-index: 0.7468 and 0.6899, respectively). Conclusions TERT C228T mutation could be a potential molecular biomarker for evaluating the prognosis of HBV-related HCC, especially when it is detected by dPCR rather than traditional Sanger sequencing. The nomogram survival prediction model we constructed may provide a valuable reference for clinicians to develop strategies for precision treatment." @default.
- W4308457286 created "2022-11-12" @default.
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- W4308457286 date "2022-11-07" @default.
- W4308457286 modified "2023-10-14" @default.
- W4308457286 title "TERT promoter mutation predicts the prognosis value of patients with HBV- related HCC who underwent hepatectomy" @default.
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- W4308457286 doi "https://doi.org/10.21203/rs.3.rs-2237837/v1" @default.
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