Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308483936> ?p ?o ?g. }
- W4308483936 abstract "Trypanosoma brucei causes human African trypanosomiasis (HAT) and nagana in cattle. During infection of a vertebrate, endocytosis of host transferrin (Tf) is important for viability of the parasite. The majority of proteins involved in trypanosome endocytosis of Tf are unknown. Here we identify pseudokinase NRP1 (Tb427tmp.160.4770) as a regulator of Tf endocytosis. Genetic knockdown of NRP1 inhibited endocytosis of Tf without blocking uptake of bovine serum albumin. Binding of Tf to the flagellar pocket was not affected by knockdown of NRP1. However the quantity of Tf per endosome dropped significantly, consistent with NRP1 promoting robust capture and/or retention of Tf in vesicles. NRP1 is involved in motility of Tf-laden vesicles since distances between endosomes and the kinetoplast were reduced after knockdown of the gene. In search of possible mediators of NRP1 modulation of Tf endocytosis, the gene was knocked down and the phosphoproteome analyzed. Phosphorylation of protein kinases forkhead, NEK6, and MAPK10 was altered, in addition to EpsinR, synaptobrevin and other vesicle-associated proteins predicted to be involved in endocytosis. These candidate proteins may link NRP1 functionally either to protein kinases or to vesicle-associated proteins." @default.
- W4308483936 created "2022-11-12" @default.
- W4308483936 creator A5009417240 @default.
- W4308483936 creator A5060265111 @default.
- W4308483936 creator A5083370650 @default.
- W4308483936 date "2022-11-03" @default.
- W4308483936 modified "2023-10-14" @default.
- W4308483936 title "Pseudokinase NRP1 facilitates endocytosis of transferrin in the African trypanosome" @default.
- W4308483936 cites W1826847771 @default.
- W4308483936 cites W1875580551 @default.
- W4308483936 cites W18982056 @default.
- W4308483936 cites W1935597412 @default.
- W4308483936 cites W1961740639 @default.
- W4308483936 cites W1963524639 @default.
- W4308483936 cites W1971203075 @default.
- W4308483936 cites W1973217987 @default.
- W4308483936 cites W1976165046 @default.
- W4308483936 cites W1981598071 @default.
- W4308483936 cites W1983916545 @default.
- W4308483936 cites W1984109928 @default.
- W4308483936 cites W1984633201 @default.
- W4308483936 cites W1986867603 @default.
- W4308483936 cites W1988588903 @default.
- W4308483936 cites W1991757639 @default.
- W4308483936 cites W1993454461 @default.
- W4308483936 cites W1994844474 @default.
- W4308483936 cites W2006453814 @default.
- W4308483936 cites W2007303185 @default.
- W4308483936 cites W2015704490 @default.
- W4308483936 cites W2018204057 @default.
- W4308483936 cites W2019196882 @default.
- W4308483936 cites W2019733207 @default.
- W4308483936 cites W2019757220 @default.
- W4308483936 cites W2022050193 @default.
- W4308483936 cites W2023863818 @default.
- W4308483936 cites W2027615173 @default.
- W4308483936 cites W2032771274 @default.
- W4308483936 cites W2033824816 @default.
- W4308483936 cites W2035270992 @default.
- W4308483936 cites W2038942939 @default.
- W4308483936 cites W2039677833 @default.
- W4308483936 cites W2039806621 @default.
- W4308483936 cites W2040645864 @default.
- W4308483936 cites W2043491287 @default.
- W4308483936 cites W2045914902 @default.
- W4308483936 cites W2047325346 @default.
- W4308483936 cites W2049645388 @default.
- W4308483936 cites W2062921399 @default.
- W4308483936 cites W2063458187 @default.
- W4308483936 cites W2065503544 @default.
- W4308483936 cites W2066432628 @default.
- W4308483936 cites W2066501531 @default.
- W4308483936 cites W2067691363 @default.
- W4308483936 cites W2070648173 @default.
- W4308483936 cites W2071555141 @default.
- W4308483936 cites W2074303585 @default.
- W4308483936 cites W2075936375 @default.
- W4308483936 cites W2079390173 @default.
- W4308483936 cites W2080659725 @default.
- W4308483936 cites W2082091164 @default.
- W4308483936 cites W2084152946 @default.
- W4308483936 cites W2085864819 @default.
- W4308483936 cites W2087051167 @default.
- W4308483936 cites W2087860032 @default.
- W4308483936 cites W2092193265 @default.
- W4308483936 cites W2098859676 @default.
- W4308483936 cites W2099244726 @default.
- W4308483936 cites W2101367227 @default.
- W4308483936 cites W2102904980 @default.
- W4308483936 cites W2109066322 @default.
- W4308483936 cites W2109360645 @default.
- W4308483936 cites W2111565312 @default.
- W4308483936 cites W2114410573 @default.
- W4308483936 cites W2116350092 @default.
- W4308483936 cites W2119367928 @default.
- W4308483936 cites W2122140053 @default.
- W4308483936 cites W2123139638 @default.
- W4308483936 cites W2124733101 @default.
- W4308483936 cites W2140452388 @default.
- W4308483936 cites W2140946052 @default.
- W4308483936 cites W2141142872 @default.
- W4308483936 cites W2141682274 @default.
- W4308483936 cites W2157236463 @default.
- W4308483936 cites W2157771983 @default.
- W4308483936 cites W2158131682 @default.
- W4308483936 cites W2166542272 @default.
- W4308483936 cites W2166572326 @default.
- W4308483936 cites W2167279371 @default.
- W4308483936 cites W2286283065 @default.
- W4308483936 cites W2305756063 @default.
- W4308483936 cites W2342008480 @default.
- W4308483936 cites W2396492116 @default.
- W4308483936 cites W2409736181 @default.
- W4308483936 cites W2434703102 @default.
- W4308483936 cites W2515773255 @default.
- W4308483936 cites W2551865313 @default.
- W4308483936 cites W2555701464 @default.
- W4308483936 cites W2592044056 @default.
- W4308483936 cites W2626832068 @default.
- W4308483936 cites W2765368183 @default.