Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308559076> ?p ?o ?g. }
- W4308559076 endingPage "90" @default.
- W4308559076 startingPage "78" @default.
- W4308559076 abstract "Conversion of CO2 to value-added products presents an opportunity to reduce GHG emissions while generating revenue. Formate, which can be generated by the electrochemical reduction of CO2, has been proposed as a promising intermediate compound for microbial upgrading. Here we present progress towards improving the soil bacterium Cupriavidus necator H16, which is capable of growing on formate as its sole source of carbon and energy using the Calvin-Benson-Bassham (CBB) cycle, as a host for formate utilization. Using adaptive laboratory evolution, we generated several isolates that exhibited faster growth rates on formate. The genomes of these isolates were sequenced, and resulting mutations were systematically reintroduced by metabolic engineering, to identify those that improved growth. The metabolic impact of several mutations was investigated further using RNA-seq transcriptomics. We found that deletion of a transcriptional regulator implicated in quorum sensing, PhcA, reduced expression of several operons and led to improved growth on formate. Growth was also improved by deleting large genomic regions present on the extrachromosomal megaplasmid pHG1, particularly two hydrogenase operons and the megaplasmid CBB operon, one of two copies present in the genome. Based on these findings, we generated a rationally engineered ΔphcA and megaplasmid-deficient strain that exhibited a 24% faster maximum growth rate on formate. Moreover, this strain achieved a 7% growth rate improvement on succinate and a 19% increase on fructose, demonstrating the broad utility of microbial genome reduction. This strain has the potential to serve as an improved microbial chassis for biological conversion of formate to value-added products." @default.
- W4308559076 created "2022-11-12" @default.
- W4308559076 creator A5000776846 @default.
- W4308559076 creator A5001661420 @default.
- W4308559076 creator A5010553008 @default.
- W4308559076 creator A5020917396 @default.
- W4308559076 creator A5047770210 @default.
- W4308559076 creator A5063550820 @default.
- W4308559076 creator A5063660547 @default.
- W4308559076 creator A5079869230 @default.
- W4308559076 date "2023-01-01" @default.
- W4308559076 modified "2023-10-14" @default.
- W4308559076 title "Improving growth of Cupriavidus necator H16 on formate using adaptive laboratory evolution-informed engineering" @default.
- W4308559076 cites W1548594428 @default.
- W4308559076 cites W1574152612 @default.
- W4308559076 cites W1581442411 @default.
- W4308559076 cites W1592029927 @default.
- W4308559076 cites W1648929910 @default.
- W4308559076 cites W1906978066 @default.
- W4308559076 cites W1941794513 @default.
- W4308559076 cites W1966116114 @default.
- W4308559076 cites W1981038407 @default.
- W4308559076 cites W1985432041 @default.
- W4308559076 cites W1990774952 @default.
- W4308559076 cites W2000993970 @default.
- W4308559076 cites W2008681679 @default.
- W4308559076 cites W2018221287 @default.
- W4308559076 cites W2029887414 @default.
- W4308559076 cites W2033809555 @default.
- W4308559076 cites W2050397787 @default.
- W4308559076 cites W2052190020 @default.
- W4308559076 cites W2056869038 @default.
- W4308559076 cites W2064815954 @default.
- W4308559076 cites W2065754101 @default.
- W4308559076 cites W2071655141 @default.
- W4308559076 cites W2075580171 @default.
- W4308559076 cites W2090284140 @default.
- W4308559076 cites W2092885921 @default.
- W4308559076 cites W2107451328 @default.
- W4308559076 cites W2107996465 @default.
- W4308559076 cites W2112012526 @default.
- W4308559076 cites W2120211414 @default.
- W4308559076 cites W2125645956 @default.
- W4308559076 cites W2128554689 @default.
- W4308559076 cites W2137673847 @default.
- W4308559076 cites W2139600238 @default.
- W4308559076 cites W2144305584 @default.
- W4308559076 cites W2152165550 @default.
- W4308559076 cites W2155639255 @default.
- W4308559076 cites W2179438025 @default.
- W4308559076 cites W2332501554 @default.
- W4308559076 cites W2396449893 @default.
- W4308559076 cites W2503176236 @default.
- W4308559076 cites W2531281183 @default.
- W4308559076 cites W2624367749 @default.
- W4308559076 cites W2734277055 @default.
- W4308559076 cites W2741548933 @default.
- W4308559076 cites W2773788583 @default.
- W4308559076 cites W2809474289 @default.
- W4308559076 cites W2897431095 @default.
- W4308559076 cites W2954701498 @default.
- W4308559076 cites W2967876620 @default.
- W4308559076 cites W2983071411 @default.
- W4308559076 cites W2983215973 @default.
- W4308559076 cites W3025836311 @default.
- W4308559076 cites W3042815916 @default.
- W4308559076 cites W3049453111 @default.
- W4308559076 cites W3087507582 @default.
- W4308559076 cites W3089714280 @default.
- W4308559076 cites W3094988616 @default.
- W4308559076 cites W3113082932 @default.
- W4308559076 cites W3127738948 @default.
- W4308559076 cites W3177576333 @default.
- W4308559076 cites W3195001954 @default.
- W4308559076 cites W3200065970 @default.
- W4308559076 cites W3206246878 @default.
- W4308559076 cites W3208259478 @default.
- W4308559076 cites W3211412751 @default.
- W4308559076 cites W4210890146 @default.
- W4308559076 cites W4241483073 @default.
- W4308559076 cites W4292608433 @default.
- W4308559076 cites W4293335046 @default.
- W4308559076 cites W4321509041 @default.
- W4308559076 cites W98006665 @default.
- W4308559076 doi "https://doi.org/10.1016/j.ymben.2022.10.016" @default.
- W4308559076 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36368470" @default.
- W4308559076 hasPublicationYear "2023" @default.
- W4308559076 type Work @default.
- W4308559076 citedByCount "6" @default.
- W4308559076 countsByYear W43085590762023 @default.
- W4308559076 crossrefType "journal-article" @default.
- W4308559076 hasAuthorship W4308559076A5000776846 @default.
- W4308559076 hasAuthorship W4308559076A5001661420 @default.
- W4308559076 hasAuthorship W4308559076A5010553008 @default.
- W4308559076 hasAuthorship W4308559076A5020917396 @default.
- W4308559076 hasAuthorship W4308559076A5047770210 @default.
- W4308559076 hasAuthorship W4308559076A5063550820 @default.
- W4308559076 hasAuthorship W4308559076A5063660547 @default.