FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308559935> ?p ?o ?g. }

• W4308559935 endingPage "167" @default.
• W4308559935 startingPage "156" @default.
• W4308559935 abstract "Focal adhesions (FAs) transmit force and mediate mechanotransduction between cells and the matrix. Previous studies revealed that integrin-transmitted force is critical to regulate FA formation. As vinculin is a prominent FA protein implicated in integrin tension transmission, this work studies the relation among integrin tensions (force), vinculin (protein), and FA formation (structure) by integrin tension manipulation, force visualization and vinculin knockout (KO). Two DNA-based integrin tension tools are adopted: tension gauge tether (TGT) and integrative tension sensor (ITS), with TGT restricting integrin tensions under a designed Ttol (tension tolerance) value and ITS visualizing integrin tensions above the Ttol value by fluorescence. Results show that large FAs (area >1 μm2) were formed on the TGT surface with Ttol of 54 pN but not on those with lower Ttol values. Time-series analysis of FA formation shows that focal complexes (area <0.5 μm2) appeared on all TGT surfaces 20 min after cell plating, but only matured to large FAs on TGT with Ttol of 54 pN. Next, we tested FA formation in vinculin KO cells on TGT surfaces. Surprisingly, the Ttol value of TGT required for large FA formation is drastically decreased to 23 pN. To explore the cause, we visualized integrin tensions in both wild-type and vinculin KO cells using ITS. The results showed that integrin tensions in FAs of wild-type cells frequently activate ITS with Ttol of 54 pN. With vinculin KO, however, integrin tensions in FAs became lower and unable to activate 54 pN ITS. Force signal intensities of integrin tensions reported by 33 and 43 pN ITS were also significantly reduced with vinculin KO, suggesting that vinculin is essential to transmit high-level integrin tensions and involved in transmitting intermediate-level integrin tensions in FAs. However, the high-level integrin tensions transmitted by vinculin are not required by FA formation." @default.
• W4308559935 created "2022-11-12" @default.
• W4308559935 creator A5015491460 @default.
• W4308559935 creator A5026018838 @default.
• W4308559935 creator A5040499570 @default.
• W4308559935 creator A5045623461 @default.
• W4308559935 date "2023-01-01" @default.
• W4308559935 modified "2023-10-12" @default.
• W4308559935 title "Vinculin transmits high-level integrin tensions that are dispensable for focal adhesion formation" @default.
• W4308559935 cites W1588280167 @default.
• W4308559935 cites W1590619267 @default.
• W4308559935 cites W1907686361 @default.
• W4308559935 cites W1964106556 @default.
• W4308559935 cites W1968940890 @default.
• W4308559935 cites W1970690847 @default.
• W4308559935 cites W1982398946 @default.
• W4308559935 cites W1999765951 @default.
• W4308559935 cites W2001129308 @default.
• W4308559935 cites W2002982362 @default.
• W4308559935 cites W2004792720 @default.
• W4308559935 cites W2007719816 @default.
• W4308559935 cites W2023392069 @default.
• W4308559935 cites W2024059382 @default.
• W4308559935 cites W2030214324 @default.
• W4308559935 cites W2038476759 @default.
• W4308559935 cites W2042936107 @default.
• W4308559935 cites W2047396661 @default.
• W4308559935 cites W2048808551 @default.
• W4308559935 cites W2054971583 @default.
• W4308559935 cites W2059583170 @default.
• W4308559935 cites W2068092779 @default.
• W4308559935 cites W2085387787 @default.
• W4308559935 cites W2085767196 @default.
• W4308559935 cites W2098518658 @default.
• W4308559935 cites W2109137926 @default.
• W4308559935 cites W2114100748 @default.
• W4308559935 cites W2115086955 @default.
• W4308559935 cites W2116527577 @default.
• W4308559935 cites W2145260575 @default.
• W4308559935 cites W2147000598 @default.
• W4308559935 cites W2149667112 @default.
• W4308559935 cites W2149873896 @default.
• W4308559935 cites W2162614357 @default.
• W4308559935 cites W2163178940 @default.
• W4308559935 cites W2328259700 @default.
• W4308559935 cites W2345307871 @default.
• W4308559935 cites W2537925581 @default.
• W4308559935 cites W2737110471 @default.
• W4308559935 cites W2751468640 @default.
• W4308559935 cites W2820932019 @default.
• W4308559935 cites W2900556494 @default.
• W4308559935 cites W2901606464 @default.
• W4308559935 cites W2953223831 @default.
• W4308559935 cites W2995123953 @default.
• W4308559935 cites W3013763111 @default.
• W4308559935 cites W3025642328 @default.
• W4308559935 cites W3164720541 @default.
• W4308559935 doi "https://doi.org/10.1016/j.bpj.2022.11.013" @default.
• W4308559935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36352785" @default.
• W4308559935 hasPublicationYear "2023" @default.
• W4308559935 type Work @default.
• W4308559935 citedByCount "5" @default.
• W4308559935 countsByYear W43085599352023 @default.
• W4308559935 crossrefType "journal-article" @default.
• W4308559935 hasAuthorship W4308559935A5015491460 @default.
• W4308559935 hasAuthorship W4308559935A5026018838 @default.
• W4308559935 hasAuthorship W4308559935A5040499570 @default.
• W4308559935 hasAuthorship W4308559935A5045623461 @default.
• W4308559935 hasConcept C1491633281 @default.
• W4308559935 hasConcept C151166631 @default.
• W4308559935 hasConcept C153911025 @default.
• W4308559935 hasConcept C181104049 @default.
• W4308559935 hasConcept C185592680 @default.
• W4308559935 hasConcept C195687474 @default.
• W4308559935 hasConcept C2777979848 @default.
• W4308559935 hasConcept C55493867 @default.
• W4308559935 hasConcept C86803240 @default.
• W4308559935 hasConcept C95444343 @default.
• W4308559935 hasConceptScore W4308559935C1491633281 @default.
• W4308559935 hasConceptScore W4308559935C151166631 @default.
• W4308559935 hasConceptScore W4308559935C153911025 @default.
• W4308559935 hasConceptScore W4308559935C181104049 @default.
• W4308559935 hasConceptScore W4308559935C185592680 @default.
• W4308559935 hasConceptScore W4308559935C195687474 @default.
• W4308559935 hasConceptScore W4308559935C2777979848 @default.
• W4308559935 hasConceptScore W4308559935C55493867 @default.
• W4308559935 hasConceptScore W4308559935C86803240 @default.
• W4308559935 hasConceptScore W4308559935C95444343 @default.
• W4308559935 hasFunder F4320306076 @default.
• W4308559935 hasFunder F4320332161 @default.
• W4308559935 hasFunder F4320337354 @default.
• W4308559935 hasIssue "1" @default.
• W4308559935 hasLocation W43085599351 @default.
• W4308559935 hasLocation W43085599352 @default.
• W4308559935 hasOpenAccess W4308559935 @default.
• W4308559935 hasPrimaryLocation W43085599351 @default.
• W4308559935 hasRelatedWork W1988590842 @default.
• W4308559935 hasRelatedWork W1996189253 @default.

• next