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- W4308562874 abstract "Synthetic single-stranded (ss) DNA is a cornerstone for life and materials science, yet the purity, quantity, length, and customizability of synthetic DNA are still limiting in various applications. Here we present PECAN, paired-end cutting assisted by DNAzymes (DNA enzymes or deoxyribozymes), which enables mass production of ssDNA of arbitrary sequence (up to 7,000 nucleotides, or nt) with single-base precision. At the core of PECAN technique are two newly-identified classes of DNAzymes, each robustly self-hydrolyzing with minimal sequence requirement up- or down-stream of its cleavage site. Flanking the target ssDNA with a pair of such DNAzymes generates a precursor ssDNA amplifiable by pseudogene-recombinant bacteriophage, which subsequently releases the target ssDNA in large quantities after efficient auto-processing. PECAN produces ssDNA of virtually any terminal bases and compositions with >98.5% purity at the milligram-to-gram scale. We demonstrate the feasibility of using PECAN ssDNA for RNA in situ detection, homology-directed genome editing, and DNA-based data storage." @default.
- W4308562874 created "2022-11-12" @default.
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- W4308562874 date "2022-12-22" @default.
- W4308562874 modified "2023-10-17" @default.
- W4308562874 title "Catalytic DNA‐Assisted Mass Production of Arbitrary Single‐Stranded DNA" @default.
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- W4308562874 doi "https://doi.org/10.1002/anie.202212011" @default.
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