FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4308571819> ?p ?o ?g. }

• W4308571819 abstract "We reported that PARP-1 regulates genes whose products are crucial for asthma, in part, by controlling STAT6 integrity speculatively through a calpain-dependent mechanism. We wished to decipher the PARP-1/STAT6 relationship in the context of intracellular trafficking and promoter occupancy of the transcription factor on target genes, its integrity in the presence of calpains, and its connection to autophagy.This study was conducted using primary splenocytes or fibroblasts derived from wild-type or PARP-1-/- mice and Jurkat T cells to mimic Th2 inflammation.We show that the role for PARP-1 in expression of IL-4-induced genes (e.g. gata-3) in splenocytes did not involve effects on STAT6 phosphorylation or its subcellular trafficking, rather, it influenced its occupancy of gata-3 proximal and distal promoters in the early stages of IL-4 stimulation. At later stages, PARP-1 was crucial for STAT6 integrity as its inhibition, pharmacologically or by gene knockout, compromised the fate of the transcription factor. Calpain-1 appeared to preferentially degrade JAK-phosphorylated-STAT6, which was blocked by calpastatin-mediated inhibition or by genetic knockout in mouse fibroblasts. The STAT6/PARP-1 relationship entailed physical interaction and modification by poly(ADP-ribosyl)ation independently of double-strand-DNA breaks. Poly(ADP-ribosyl)ation protected phosphorylated-STAT6 against calpain-1-mediated degradation. Additionally, our results show that STAT6 is a bonafide substrate for chaperone-mediated autophagy in a selective and calpain-dependent manner in the human Jurkat cell-line. The effects were partially blocked by IL-4 treatment and PARP-1 inhibition.The results demonstrate that poly(ADP-ribosyl)ation plays a critical role in protecting activated STAT6 during Th2 inflammation, which may be synthetically targeted for degradation by inhibiting PARP-1." @default.
• W4308571819 created "2022-11-12" @default.
• W4308571819 creator A5016671074 @default.
• W4308571819 creator A5020916102 @default.
• W4308571819 creator A5035463544 @default.
• W4308571819 creator A5036846597 @default.
• W4308571819 creator A5047070829 @default.
• W4308571819 creator A5079091531 @default.
• W4308571819 creator A5086272919 @default.
• W4308571819 date "2022-11-08" @default.
• W4308571819 modified "2023-10-18" @default.
• W4308571819 title "Promotion of a synthetic degradation of activated STAT6 by PARP-1 inhibition: roles of poly(ADP-ribosyl)ation, calpains and autophagy" @default.
• W4308571819 cites W1045337831 @default.
• W4308571819 cites W1506161763 @default.
• W4308571819 cites W1515402682 @default.
• W4308571819 cites W1590261010 @default.
• W4308571819 cites W1619362288 @default.
• W4308571819 cites W1639940444 @default.
• W4308571819 cites W1774217174 @default.
• W4308571819 cites W1969078294 @default.
• W4308571819 cites W1980528721 @default.
• W4308571819 cites W1982451689 @default.
• W4308571819 cites W2010190752 @default.
• W4308571819 cites W2013131852 @default.
• W4308571819 cites W2021919397 @default.
• W4308571819 cites W2022964430 @default.
• W4308571819 cites W2027699993 @default.
• W4308571819 cites W2035572991 @default.
• W4308571819 cites W2038540586 @default.
• W4308571819 cites W2040414192 @default.
• W4308571819 cites W2045631079 @default.
• W4308571819 cites W2047281917 @default.
• W4308571819 cites W2048489471 @default.
• W4308571819 cites W2053942643 @default.
• W4308571819 cites W2055834907 @default.
• W4308571819 cites W2062142744 @default.
• W4308571819 cites W2062747680 @default.
• W4308571819 cites W2065247148 @default.
• W4308571819 cites W2069509382 @default.
• W4308571819 cites W2088059945 @default.
• W4308571819 cites W2089445890 @default.
• W4308571819 cites W2096529411 @default.
• W4308571819 cites W2098561880 @default.
• W4308571819 cites W2104519081 @default.
• W4308571819 cites W2105803506 @default.
• W4308571819 cites W2106104034 @default.
• W4308571819 cites W2123326944 @default.
• W4308571819 cites W2124645898 @default.
• W4308571819 cites W2131556221 @default.
• W4308571819 cites W2133893126 @default.
• W4308571819 cites W2146248342 @default.
• W4308571819 cites W2147119127 @default.
• W4308571819 cites W2148339508 @default.
• W4308571819 cites W2149061302 @default.
• W4308571819 cites W2165096527 @default.
• W4308571819 cites W2171449633 @default.
• W4308571819 cites W2404157374 @default.
• W4308571819 cites W2460417987 @default.
• W4308571819 cites W2553989095 @default.
• W4308571819 cites W2752326209 @default.
• W4308571819 cites W2765136070 @default.
• W4308571819 cites W2782355556 @default.
• W4308571819 cites W2888992248 @default.
• W4308571819 cites W2913985159 @default.
• W4308571819 cites W2982554531 @default.
• W4308571819 cites W2983017086 @default.
• W4308571819 cites W2988338102 @default.
• W4308571819 cites W3099583015 @default.
• W4308571819 cites W3121181609 @default.
• W4308571819 cites W3158269582 @default.
• W4308571819 cites W4210628738 @default.
• W4308571819 cites W4256317085 @default.
• W4308571819 doi "https://doi.org/10.1186/s12967-022-03715-x" @default.
• W4308571819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36348405" @default.
• W4308571819 hasPublicationYear "2022" @default.
• W4308571819 type Work @default.
• W4308571819 citedByCount "0" @default.
• W4308571819 crossrefType "journal-article" @default.
• W4308571819 hasAuthorship W4308571819A5016671074 @default.
• W4308571819 hasAuthorship W4308571819A5020916102 @default.
• W4308571819 hasAuthorship W4308571819A5035463544 @default.
• W4308571819 hasAuthorship W4308571819A5036846597 @default.
• W4308571819 hasAuthorship W4308571819A5047070829 @default.
• W4308571819 hasAuthorship W4308571819A5079091531 @default.
• W4308571819 hasAuthorship W4308571819A5086272919 @default.
• W4308571819 hasBestOaLocation W43085718191 @default.
• W4308571819 hasConcept C101762097 @default.
• W4308571819 hasConcept C104317684 @default.
• W4308571819 hasConcept C145430368 @default.
• W4308571819 hasConcept C150194340 @default.
• W4308571819 hasConcept C153911025 @default.
• W4308571819 hasConcept C179464577 @default.
• W4308571819 hasConcept C181199279 @default.
• W4308571819 hasConcept C182979987 @default.
• W4308571819 hasConcept C185592680 @default.
• W4308571819 hasConcept C2775975398 @default.
• W4308571819 hasConcept C2776090121 @default.
• W4308571819 hasConcept C54355233 @default.
• W4308571819 hasConcept C55493867 @default.
• W4308571819 hasConcept C56928146 @default.

• next