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- W4308583764 abstract "The ADAPT (Assisted Design of Antibody and Protein Therapeutics) platform guides the selection of mutants that improve/modulate the affinity of antibodies and other biologics. Predicted affinities are based on a consensus z-score from three scoring functions. Computational predictions are interleaved with experimental validation, significantly enhancing the robustness of the design and selection of mutants. A key step is an initial exhaustive virtual single-mutant scan that identifies hot spots and the mutations predicted to improve affinity. A small number of proposed single mutants are then produced and assayed. Only the validated single mutants (i.e., having improved affinity) are used to design double and higher-order mutants in subsequent rounds of design, avoiding the combinatorial explosion that arises from random mutagenesis. Typically, with a total of about 30–50 designed single, double, and triple mutants, affinity improvements of 10- to 100-fold are obtained." @default.
- W4308583764 created "2022-11-12" @default.
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- W4308583764 date "2022-11-09" @default.
- W4308583764 modified "2023-10-17" @default.
- W4308583764 title "Optimizing Antibody–Antigen Binding Affinities with the ADAPT Platform" @default.
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- W4308583764 doi "https://doi.org/10.1007/978-1-0716-2609-2_20" @default.
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